Detailed information NTG0399915

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: GJD98_RS04685
• Genome accession: NZ_CP045974
• Coordinates: 1013636..1014700 (+)
• Length: 354 a.a.
• NCBI ID: WP_010946662.1
• Uniprot ID: Q5ZX06
• Organism: Legionella pneumophila strain AUSMDU00010536 isolate SBT211
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1008636..1019700

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GJD98_RS04675 (GJD98_04675)	-	1009673..1012057 (-)	2385	WP_010946660.1	penicillin-binding protein 1A	-
GJD98_RS04680 (GJD98_04680)	ravI	1012209..1013219 (-)	1011	WP_010946661.1	Dot/Icm T4SS effector RavI	-
GJD98_RS04685 (GJD98_04685)	pilM	1013636..1014700 (+)	1065	WP_010946662.1	type IV pilus assembly protein PilM	Machinery gene
GJD98_RS04690 (GJD98_04690)	pilN	1014704..1015252 (+)	549	WP_010946663.1	PilN domain-containing protein	Machinery gene
GJD98_RS04695 (GJD98_04695)	pilO	1015266..1015865 (+)	600	WP_014841277.1	type 4a pilus biogenesis protein PilO	Machinery gene
GJD98_RS04700 (GJD98_04700)	pilP	1015862..1016449 (+)	588	WP_010946665.1	pilus assembly protein PilP	Machinery gene
GJD98_RS04705 (GJD98_04705)	pilQ	1016453..1018549 (+)	2097	WP_010946666.1	type IV pilus secretin PilQ	Machinery gene
GJD98_RS04710 (GJD98_04710)	aroK	1018995..1019522 (+)	528	WP_010946667.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38732.71 Da
• Isoelectric Point: 5.0367

>NTDB_id=399915 GJD98_RS04685 WP_010946662.1 1013636..1014700(+) (pilM) [Legionella pneumophila strain AUSMDU00010536 isolate SBT211]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNIIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQSGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide

• Length: 1065 bp

>NTDB_id=399915 GJD98_RS04685 WP_010946662.1 1013636..1014700(+) (pilM) [Legionella pneumophila strain AUSMDU00010536 isolate SBT211]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATCCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGCGAAGAAATACCTGCCTCTGCCTTAGAAGGAAATATAA
TTAAGGATGTCGATGTAGTAGCGAATTGTATCAAAAAATTAGTAGAAAGATTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTGGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAGTCTTATGCAGTAGAGCGTTCAGTACAACAAATCGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAATTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAACACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGGCAATCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCGCCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-352)

3D structure

• AlphaFold DB: Q5ZX06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Legionella pneumophila strain ERS1305867	99.435	100	0.994
comM	Acinetobacter nosocomialis M2	42.817	100	0.429
pilM	Acinetobacter baumannii D1279779	42.535	100	0.427
comM	Acinetobacter baylyi ADP1	41.457	100	0.418