Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPE49_RS11670 Genome accession   NZ_CP054363
Coordinates   2408316..2409797 (+) Length   493 a.a.
NCBI ID   WP_001219896.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-171     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2403316..2414797
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE49_RS11650 (HPE49_11645) gspB 2403485..2403904 (-) 420 WP_000461444.1 putative general secretion pathway protein GspB -
  HPE49_RS11655 (HPE49_11650) gspA 2403906..2405375 (-) 1470 WP_000107581.1 type II secretion system protein GspA -
  HPE49_RS11660 (HPE49_11655) gspC 2405555..2406370 (+) 816 WP_001142702.1 type II secretion system protein GspC -
  HPE49_RS11665 (HPE49_11660) gspD 2406354..2408306 (+) 1953 WP_001562179.1 type II secretion system secretin GspD -
  HPE49_RS11670 (HPE49_11665) pilB 2408316..2409797 (+) 1482 WP_001219896.1 type II secretion system ATPase GspE Machinery gene
  HPE49_RS11675 (HPE49_11670) gspF 2409794..2410990 (+) 1197 WP_001109559.1 type II secretion system inner membrane protein GspF -
  HPE49_RS11680 (HPE49_11675) gspG 2411000..2411437 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HPE49_RS11685 (HPE49_11680) gspH 2411445..2411954 (+) 510 WP_001076031.1 type II secretion system minor pseudopilin GspH -
  HPE49_RS11690 (HPE49_11685) gspI 2411951..2412328 (+) 378 WP_001041738.1 type II secretion system minor pseudopilin GspI -
  HPE49_RS11695 (HPE49_11690) gspJ 2412321..2412908 (+) 588 WP_000610629.1 type II secretion system minor pseudopilin GspJ -
  HPE49_RS11700 (HPE49_11695) gspK 2412901..2413884 (+) 984 WP_001058528.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54623.52 Da        Isoelectric Point: 6.1000

>NTDB_id=399042 HPE49_RS11670 WP_001219896.1 2408316..2409797(+) (pilB) [Escherichia coli strain SCU-171]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVISGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=399042 HPE49_RS11670 WP_001219896.1 2408316..2409797(+) (pilB) [Escherichia coli strain SCU-171]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGACGTATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATCCAGCTT
CCACACGGTATTATCCTGGTGACCGGACCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Acinetobacter baumannii D1279779

45.805

89.452

0.41
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361