Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HPE50_RS21410 Genome accession   NZ_CP054353
Coordinates   4317228..4318721 (-) Length   497 a.a.
NCBI ID   WP_001530857.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-172     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4312228..4323721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE50_RS21380 (HPE50_21375) gspK 4313022..4313999 (-) 978 WP_000633184.1 type II secretion system minor pseudopilin GspK -
  HPE50_RS21385 (HPE50_21380) gspJ 4313996..4314601 (-) 606 WP_001350472.1 type II secretion system minor pseudopilin GspJ -
  HPE50_RS21390 (HPE50_21385) gspI 4314598..4314969 (-) 372 WP_001530855.1 type II secretion system minor pseudopilin GspI -
  HPE50_RS21395 (HPE50_21390) gspH 4314966..4315529 (-) 564 WP_001115121.1 type II secretion system minor pseudopilin GspH -
  HPE50_RS21400 (HPE50_21395) gspG 4315533..4315988 (-) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HPE50_RS21405 (HPE50_21400) gspF 4316005..4317228 (-) 1224 WP_001173446.1 type II secretion system inner membrane protein GspF -
  HPE50_RS21410 (HPE50_21405) pilB/pilB1 4317228..4318721 (-) 1494 WP_001530857.1 type II secretion system ATPase GspE Machinery gene
  HPE50_RS21415 (HPE50_21410) gspD 4318721..4320781 (-) 2061 WP_001468189.1 type II secretion system secretin GspD -
  HPE50_RS21420 (HPE50_21415) gspC 4320811..4321770 (-) 960 WP_001296397.1 type II secretion system protein GspC -
  HPE50_RS21425 (HPE50_21420) gspS2 4321788..4322198 (-) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  HPE50_RS21430 (HPE50_21425) pilD 4322264..4323073 (-) 810 WP_001296399.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55180.33 Da        Isoelectric Point: 5.9081

>NTDB_id=398992 HPE50_RS21410 WP_001530857.1 4317228..4318721(-) (pilB/pilB1) [Escherichia coli strain SCU-172]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=398992 HPE50_RS21410 WP_001530857.1 4317228..4318721(-) (pilB/pilB1) [Escherichia coli strain SCU-172]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGCCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTACGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment