Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HPE52_RS20745 Genome accession   NZ_CP054345
Coordinates   4283351..4284832 (+) Length   493 a.a.
NCBI ID   WP_097430634.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-176     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4278351..4289832
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPE52_RS20725 (HPE52_20715) gspB 4278520..4278939 (-) 420 WP_000461443.1 putative general secretion pathway protein GspB -
  HPE52_RS20730 (HPE52_20720) gspA 4278941..4280410 (-) 1470 WP_000107589.1 type II secretion system protein GspA -
  HPE52_RS20735 (HPE52_20725) gspC 4280590..4281405 (+) 816 WP_001142674.1 type II secretion system protein GspC -
  HPE52_RS20740 (HPE52_20730) gspD 4281389..4283341 (+) 1953 WP_001525807.1 type II secretion system secretin GspD -
  HPE52_RS20745 (HPE52_20735) pilB 4283351..4284832 (+) 1482 WP_097430634.1 type II secretion system ATPase GspE Machinery gene
  HPE52_RS20750 (HPE52_20740) gspF 4284829..4286025 (+) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  HPE52_RS20755 (HPE52_20745) gspG 4286035..4286472 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HPE52_RS20760 (HPE52_20750) gspH 4286480..4286991 (+) 512 Protein_4107 type II secretion system minor pseudopilin GspH -
  HPE52_RS20765 (HPE52_20755) gspI 4286988..4287365 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  HPE52_RS20770 (HPE52_20760) gspJ 4287358..4287945 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  HPE52_RS20775 (HPE52_20765) gspK 4287938..4288921 (+) 984 WP_001058513.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54664.57 Da        Isoelectric Point: 6.1000

>NTDB_id=398903 HPE52_RS20745 WP_097430634.1 4283351..4284832(+) (pilB) [Escherichia coli strain SCU-176]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFEQLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=398903 HPE52_RS20745 WP_097430634.1 4283351..4284832(+) (pilB) [Escherichia coli strain SCU-176]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACAGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCGCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGTTGCCCTCATTGCCATCAATCCGGTTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.58

97.566

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361