Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HRS05_RS08270 Genome accession   NZ_CP054161
Coordinates   1743806..1745299 (+) Length   497 a.a.
NCBI ID   WP_000249328.1    Uniprot ID   -
Organism   Escherichia coli strain STO_Bone2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1738806..1750299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRS05_RS08250 (HRS05_08175) pilD 1739454..1740263 (+) 810 WP_029365213.1 prepilin peptidase PppA Machinery gene
  HRS05_RS08255 (HRS05_08180) gspS2 1740329..1740739 (+) 411 WP_001324279.1 type II secretion system pilot lipoprotein GspS-beta -
  HRS05_RS08260 (HRS05_08185) gspC 1740757..1741716 (+) 960 WP_024166543.1 type II secretion system protein GspC -
  HRS05_RS08265 (HRS05_08190) gspD 1741746..1743806 (+) 2061 WP_057950701.1 type II secretion system secretin GspD -
  HRS05_RS08270 (HRS05_08195) pilB/pilB1 1743806..1745299 (+) 1494 WP_000249328.1 type II secretion system ATPase GspE Machinery gene
  HRS05_RS08275 (HRS05_08200) gspF 1745299..1746522 (+) 1224 WP_001173413.1 type II secretion system inner membrane protein GspF -
  HRS05_RS08280 (HRS05_08205) gspG 1746539..1746994 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HRS05_RS08285 (HRS05_08210) gspH 1746998..1747561 (+) 564 WP_057950702.1 type II secretion system minor pseudopilin GspH -
  HRS05_RS08290 (HRS05_08215) gspI 1747558..1747929 (+) 372 WP_000820120.1 type II secretion system minor pseudopilin GspI -
  HRS05_RS08295 (HRS05_08220) gspJ 1747926..1748531 (+) 606 WP_001350472.1 type II secretion system minor pseudopilin GspJ -
  HRS05_RS08300 (HRS05_08225) gspK 1748528..1749505 (+) 978 WP_001556239.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55190.29 Da        Isoelectric Point: 6.0084

>NTDB_id=397620 HRS05_RS08270 WP_000249328.1 1743806..1745299(+) (pilB/pilB1) [Escherichia coli strain STO_Bone2]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIDGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=397620 HRS05_RS08270 WP_000249328.1 1743806..1745299(+) (pilB/pilB1) [Escherichia coli strain STO_Bone2]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTTGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCTACCATGCCTTCTGCCTGGGGGG
AACGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTTGGTTTGAGTCACGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
ACGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTAGGAATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTAGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362