Detailed information NTG0397126

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: GFB99_RS19995
• Genome accession: NZ_CP045782
• Coordinates: 4159338..4160138 (-)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain LEMB18
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4154338..4165138

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFB99_RS19975 (GFB99_19975)	dksA	4154918..4155373 (-)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-
GFB99_RS19980 (GFB99_19980)	sfsA	4155536..4156252 (-)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
GFB99_RS19985 (GFB99_19985)	thpR	4156252..4156788 (-)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
GFB99_RS19990 (GFB99_19990)	hrpB	4156861..4159290 (+)	2430	WP_064145607.1	ATP-dependent helicase HrpB	-
GFB99_RS19995 (GFB99_19995)	pilD	4159338..4160138 (-)	801	WP_004145898.1	prepilin peptidase	Machinery gene
GFB99_RS20000 (GFB99_20000)	-	4160135..4160881 (-)	747	WP_004145897.1	type II secretion system protein N	-
GFB99_RS20005 (GFB99_20005)	-	4160871..4161356 (-)	486	WP_004145896.1	type II secretion system protein M	-
GFB99_RS20010 (GFB99_20010)	gspL	4161349..4162542 (-)	1194	WP_004191957.1	type II secretion system protein GspL	-
GFB99_RS20015 (GFB99_20015)	gspK	4162529..4163515 (-)	987	WP_021467522.1	type II secretion system minor pseudopilin GspK	-
GFB99_RS20020 (GFB99_20020)	gspJ	4163512..4164108 (-)	597	WP_023286045.1	type II secretion system minor pseudopilin GspJ	-
GFB99_RS20025 (GFB99_20025)	gspI	4164105..4164470 (-)	366	WP_065811636.1	type II secretion system minor pseudopilin GspI	-
GFB99_RS20030 (GFB99_20030)	gspH	4164467..4164982 (-)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=397126 GFB99_RS19995 WP_004145898.1 4159338..4160138(-) (pilD) [Klebsiella pneumoniae strain LEMB18]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=397126 GFB99_RS19995 WP_004145898.1 4159338..4160138(-) (pilD) [Klebsiella pneumoniae strain LEMB18]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGTCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425