Detailed information NTG0395500

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: GFB63_RS10030
• Genome accession: NZ_CP045596
• Coordinates: 1944389..1945228 (-)
• Length: 279 a.a.
• NCBI ID: WP_011225887.1
• Uniprot ID: Q5M4N4
• Organism: Streptococcus thermophilus strain TK-P3A
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1939389..1950228

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GFB63_RS10015 (GFB63_10280)	-	1940447..1941106 (-)	660	WP_084825952.1	response regulator transcription factor	-
GFB63_RS10020 (GFB63_10285)	-	1941142..1941966 (-)	825	WP_011681087.1	TraX family protein	-
GFB63_RS10025 (GFB63_10290)	topA	1942083..1944221 (-)	2139	WP_084825951.1	type I DNA topoisomerase	-
GFB63_RS10030 (GFB63_10295)	dprA	1944389..1945228 (-)	840	WP_011225887.1	DNA-processing protein DprA	Machinery gene
GFB63_RS10035 (GFB63_10300)	-	1945295..1946215 (-)	921	WP_193838295.1	ROK family protein	-
GFB63_RS10040 (GFB63_10305)	-	1946226..1946993 (-)	768	WP_082245791.1	ribonuclease HII	-
GFB63_RS10045 (GFB63_10310)	ylqF	1946980..1947834 (-)	855	WP_084828773.1	ribosome biogenesis GTPase YlqF	-
GFB63_RS10050 (GFB63_10315)	-	1947962..1948651 (-)	690	WP_046206437.1	helix-turn-helix transcriptional regulator	-
GFB63_RS10055 (GFB63_10320)	-	1948746..1949261 (-)	516	WP_084828772.1	transcription repressor NadR	-
GFB63_RS10060 (GFB63_10325)	-	1949285..1949812 (-)	528	WP_193838296.1	ECF transporter S component	-

Sequence

Protein

• Length: 279 a.a.
• Molecular weight: 31247.18 Da
• Isoelectric Point: 8.6571

>NTDB_id=395500 GFB63_RS10030 WP_011225887.1 1944389..1945228(-) (dprA) [Streptococcus thermophilus strain TK-P3A]
MNNFELFKLKAAGLTNLNILNILDYQKNQDKKLSLRDMAVVSKSKNAVLFMEKYKNLDVKQLKEDFQRFPTLSIFDKEYP
IELKHSYNPPVLLFYQGNIDLLSKPKLAVVGARKCSETGKQSVRKIVHELGNAFTIVSGLARGIDTCAHMEALKNKGNTI
AVIGTGLDVYYPKENKALQDYMAKNHLVLTEYGPGEQPLKYHYPERNRIISGLCQGVMVVEAKLRSGSLITCERAMEEGR
DVFAIPGNILDGKSDGCHHLIQEGAKCITTGFDIISEFS

Nucleotide

• Length: 840 bp

>NTDB_id=395500 GFB63_RS10030 WP_011225887.1 1944389..1945228(-) (dprA) [Streptococcus thermophilus strain TK-P3A]
ATGAATAACTTTGAACTTTTTAAACTAAAAGCTGCTGGATTGACGAATTTAAACATCCTTAACATTTTAGACTATCAAAA
AAATCAAGATAAAAAATTGAGCTTACGTGATATGGCAGTGGTATCTAAGTCTAAAAATGCCGTTCTCTTTATGGAAAAAT
ACAAGAATTTGGATGTTAAGCAGCTTAAGGAAGATTTTCAACGTTTTCCGACCTTATCAATTTTTGATAAGGAGTATCCC
ATTGAGCTAAAACATTCCTATAATCCTCCTGTTCTCCTCTTTTATCAAGGGAATATTGATTTGCTAAGTAAACCCAAGTT
AGCAGTAGTTGGTGCCAGAAAGTGTTCTGAAACAGGCAAACAGTCTGTTCGAAAAATTGTTCATGAACTTGGGAATGCCT
TTACTATTGTAAGTGGCTTGGCGCGTGGAATTGATACTTGTGCTCATATGGAGGCCTTAAAAAATAAAGGTAACACCATT
GCAGTTATCGGGACTGGGCTAGACGTATATTATCCTAAAGAAAACAAAGCTTTACAGGACTATATGGCTAAAAATCATTT
AGTTCTTACAGAATACGGACCGGGTGAACAGCCACTTAAATACCATTATCCTGAACGAAATCGGATAATTTCTGGATTGT
GTCAAGGGGTTATGGTGGTTGAAGCCAAGCTTCGCTCGGGAAGTCTTATTACTTGTGAGCGTGCAATGGAAGAAGGAAGA
GATGTTTTCGCCATACCAGGAAATATTTTAGATGGAAAATCGGATGGATGCCATCACCTCATTCAAGAGGGCGCAAAGTG
CATTACTACAGGATTTGACATCATTTCAGAATTTTCATAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (71-269)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-62)

3D structure

• AlphaFold DB: Q5M4N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	72.043	100	0.72
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae D39	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae R6	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	63.309	99.642	0.631
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	62.95	99.642	0.627
dprA/cilB/dalA	Streptococcus mitis SK321	62.95	99.642	0.627
dprA	Lactococcus lactis subsp. cremoris KW2	60.432	99.642	0.602
dprA	Latilactobacillus sakei subsp. sakei 23K	39.768	92.832	0.369
dprA	Bacillus subtilis subsp. subtilis str. 168	48.095	75.269	0.362