Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   GFB63_RS08570 Genome accession   NZ_CP045596
Coordinates   1669787..1670461 (+) Length   224 a.a.
NCBI ID   WP_084828742.1    Uniprot ID   A0A0E2Q1N7
Organism   Streptococcus thermophilus strain TK-P3A     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1664787..1675461
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GFB63_RS08550 (GFB63_08780) - 1665916..1666488 (-) 573 WP_002948204.1 ECF transporter S component -
  GFB63_RS08555 (GFB63_08785) coaC 1666516..1667061 (-) 546 WP_002950459.1 phosphopantothenoylcysteine decarboxylase -
  GFB63_RS08560 (GFB63_08790) - 1667054..1667737 (-) 684 WP_084828741.1 phosphopantothenate--cysteine ligase -
  GFB63_RS08565 (GFB63_08795) - 1667931..1669601 (+) 1671 WP_024704082.1 formate--tetrahydrofolate ligase -
  GFB63_RS08570 (GFB63_08800) ciaR 1669787..1670461 (+) 675 WP_084828742.1 response regulator transcription factor Regulator
  GFB63_RS11180 (GFB63_08805) - 1670451..1670690 (+) 240 WP_409350141.1 histidine kinase -
  GFB63_RS10755 (GFB63_08815) - 1670835..1671871 (+) 1037 Protein_1656 sensor histidine kinase -
  GFB63_RS08590 (GFB63_08820) rpsT 1672220..1672456 (-) 237 WP_084825632.1 30S ribosomal protein S20 -
  GFB63_RS08595 (GFB63_08825) coaA 1672525..1673445 (-) 921 WP_023909573.1 type I pantothenate kinase -
  GFB63_RS08600 (GFB63_08830) - 1673768..1674358 (+) 591 WP_193838248.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 224 a.a.        Molecular weight: 25404.92 Da        Isoelectric Point: 4.1229

>NTDB_id=395494 GFB63_RS08570 WP_084828742.1 1669787..1670461(+) (ciaR) [Streptococcus thermophilus strain TK-P3A]
MIKILLVEDDLSLSNSVFDFLDDFADVMQVFDGDEGLYEAESGVYDLILLDLMLPEKDGFTVLKELREQGITTPVLIMTA
KESLDDKGHGFELGADDYLTKPFYLEELKMRIQALLKRSGKFDQNTFSFGDVCVNLSTNSTYVGDEEVELLGKEFDLLVY
FLQNQNVILPKSQIFDRLWGFDSDTNVSVVEVYVSKIRKKLKGTDFATNLQTLRSVGYILKNAD

Nucleotide


Download         Length: 675 bp        

>NTDB_id=395494 GFB63_RS08570 WP_084828742.1 1669787..1670461(+) (ciaR) [Streptococcus thermophilus strain TK-P3A]
ATGATTAAAATTTTACTAGTAGAAGATGATTTGAGTTTGTCGAACTCAGTTTTTGATTTTTTAGATGACTTTGCGGATGT
GATGCAAGTATTTGATGGAGATGAAGGTCTTTACGAAGCTGAAAGTGGTGTTTATGATTTGATTCTTTTGGACTTAATGT
TACCTGAAAAAGATGGTTTTACAGTTCTTAAAGAATTGCGTGAACAAGGAATAACGACTCCTGTTCTTATCATGACTGCT
AAGGAAAGTCTAGACGATAAGGGGCATGGCTTCGAACTCGGAGCTGATGATTATCTAACCAAGCCTTTCTATTTAGAAGA
ATTGAAGATGCGTATTCAGGCACTTTTGAAACGTTCTGGTAAATTTGATCAAAATACTTTTTCTTTTGGAGATGTTTGTG
TCAACCTTTCTACAAATTCAACTTATGTGGGTGATGAAGAAGTAGAACTTCTTGGTAAAGAATTTGATTTATTAGTCTAC
TTCCTACAAAATCAAAACGTTATTTTGCCCAAGTCGCAAATCTTTGATCGTCTTTGGGGATTTGATAGTGATACCAATGT
CTCTGTTGTTGAAGTTTATGTTTCGAAAATTCGTAAGAAACTTAAGGGGACTGATTTTGCTACCAACCTACAAACCTTAA
GAAGTGTGGGGTATATTTTGAAGAATGCTGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E2Q1N7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

87.444

99.554

0.871
  ciaR Streptococcus pneumoniae D39

87.444

99.554

0.871
  ciaR Streptococcus pneumoniae R6

87.444

99.554

0.871
  ciaR Streptococcus pneumoniae TIGR4

87.444

99.554

0.871
  ciaR Streptococcus mutans UA159

85.268

100

0.853
  covR Lactococcus lactis subsp. lactis strain DGCC12653

36.889

100

0.371
  vicR Streptococcus mutans UA159

34.746

100

0.366

Multiple sequence alignment