Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HPY15_RS02330 Genome accession   NZ_CP053800
Coordinates   486056..486526 (-) Length   156 a.a.
NCBI ID   WP_046127050.1    Uniprot ID   -
Organism   Vibrio cholerae strain SO5Y     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481056..491526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY15_RS02310 (HPY15_02310) coaE 481538..482146 (-) 609 WP_175235540.1 dephospho-CoA kinase -
  HPY15_RS02315 (HPY15_02315) pilD 482143..483018 (-) 876 WP_046127053.1 A24 family peptidase Machinery gene
  HPY15_RS02320 (HPY15_02320) pilC 483076..484302 (-) 1227 WP_046127052.1 type II secretion system F family protein Machinery gene
  HPY15_RS02325 (HPY15_02325) pilB 484345..486033 (-) 1689 WP_046127051.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HPY15_RS02330 (HPY15_02330) pilA 486056..486526 (-) 471 WP_046127050.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HPY15_RS02335 (HPY15_02335) nadC 486771..487661 (-) 891 WP_000665274.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HPY15_RS02340 (HPY15_02340) ampD 487957..488502 (+) 546 WP_175235542.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HPY15_RS02345 (HPY15_02345) - 488669..490057 (-) 1389 WP_001959226.1 IS4-like element ISVa18 family transposase -
  HPY15_RS02350 (HPY15_02350) fldB 490196..490714 (-) 519 WP_019828645.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 15876.85 Da        Isoelectric Point: 6.9457

>NTDB_id=394947 HPY15_RS02330 WP_046127050.1 486056..486526(-) (pilA) [Vibrio cholerae strain SO5Y]
MKAYKNKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSEVASAVASLKSIITPAELHYQEYGAFPNDSTELERVGV
GSNAVKNGTLTFNKTAGTITLALASPSGVSAIITRDGSTGWSCAVTGLTNQSNAPKGCPQSDSGTGGTGDTGGSGS

Nucleotide


Download         Length: 471 bp        

>NTDB_id=394947 HPY15_RS02330 WP_046127050.1 486056..486526(-) (pilA) [Vibrio cholerae strain SO5Y]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTAGTGGCGATTATTGGTGTGTT
GTCAGCAATTGCTGTACCAGCTTATAAAGACTACGTAACCAAAAGTGAAGTGGCTTCTGCGGTAGCATCTTTAAAATCGA
TTATTACTCCAGCAGAACTTCATTACCAAGAATATGGAGCCTTCCCAAATGACAGCACAGAATTAGAAAGAGTCGGAGTA
GGCTCTAATGCTGTCAAAAATGGTACATTAACATTTAACAAAACAGCAGGAACTATAACTTTAGCATTAGCCTCACCTTC
AGGAGTATCAGCAATTATTACGAGAGATGGTTCAACAGGTTGGTCATGTGCTGTAACAGGTCTAACCAACCAGAGTAATG
CACCTAAAGGCTGTCCTCAATCGGATAGCGGCACTGGTGGCACTGGCGACACTGGTGGCTCTGGTAGTTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

51.562

82.051

0.423
  pilA Pseudomonas aeruginosa PAK

40.268

95.513

0.385
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.065

99.359

0.378
  pilA Vibrio cholerae strain A1552

38.065

99.359

0.378
  pilA Vibrio cholerae C6706

38.065

99.359

0.378