Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FIU98_RS12850 Genome accession   NZ_CP045359
Coordinates   2760128..2761348 (-) Length   406 a.a.
NCBI ID   WP_172979150.1    Uniprot ID   -
Organism   Pseudomonas sp. THAF42     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2755128..2766348
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIU98_RS12835 (FIU98_12780) - 2756178..2757077 (+) 900 WP_152488826.1 hydroxymethylglutaryl-CoA lyase -
  FIU98_RS12840 (FIU98_12785) - 2757149..2759104 (+) 1956 WP_152488827.1 acetoacetate--CoA ligase -
  FIU98_RS12845 (FIU98_12790) - 2759373..2760131 (+) 759 WP_152488828.1 ABC transporter substrate-binding protein -
  FIU98_RS12850 (FIU98_12795) pilU 2760128..2761348 (-) 1221 WP_172979150.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FIU98_RS12855 (FIU98_12800) - 2761497..2761775 (+) 279 WP_013715799.1 peptidylprolyl isomerase -
  FIU98_RS12860 (FIU98_12805) - 2761783..2762694 (-) 912 WP_152488829.1 LysR family transcriptional regulator -
  FIU98_RS12865 (FIU98_12810) - 2762760..2763239 (-) 480 WP_047591574.1 nuclear transport factor 2 family protein -
  FIU98_RS12870 (FIU98_12815) - 2763274..2763840 (-) 567 WP_037047867.1 TetR/AcrR family transcriptional regulator -
  FIU98_RS12875 (FIU98_12820) - 2763955..2764875 (-) 921 WP_152488830.1 LysR family transcriptional regulator -
  FIU98_RS12880 (FIU98_12825) - 2765017..2765412 (+) 396 WP_037047879.1 RidA family protein -
  FIU98_RS12885 (FIU98_12830) nfsB 2765446..2766096 (-) 651 WP_152488831.1 oxygen-insensitive NAD(P)H nitroreductase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44686.05 Da        Isoelectric Point: 5.7269

>NTDB_id=393645 FIU98_RS12850 WP_172979150.1 2760128..2761348(-) (pilU) [Pseudomonas sp. THAF42]
MDLPAMLKVLSTQDGSDLYLSTGAPPCAKFNGVLKALSQEPLKAGDVAAIADSVMDSAQREEFERELEMNLAISLAGVGR
FRINIFKQRNEVSIVARNIKMEIPRFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTIDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHDGAIDEEEAVKNADSANNVRLKLKLYRDNPANAKPAPVAAAPVAAPAAAPKVAEAGDWGLELKLEDIEEEQPPED
PGRQGI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=393645 FIU98_RS12850 WP_172979150.1 2760128..2761348(-) (pilU) [Pseudomonas sp. THAF42]
ATGGATCTCCCTGCAATGCTGAAAGTCCTGTCCACGCAGGACGGTTCCGACCTCTATCTCTCCACCGGCGCGCCGCCCTG
TGCCAAGTTCAATGGCGTGCTCAAGGCGCTCAGCCAGGAGCCGCTGAAGGCGGGGGACGTGGCCGCCATCGCGGATTCGG
TCATGGATAGCGCGCAGCGCGAGGAGTTCGAGCGCGAACTGGAAATGAACCTGGCGATCTCGCTGGCCGGGGTCGGTCGT
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGATGGAGATTCCACGCTTCGA
AGACCTCAAGCTGCCCGAGGTCCTGCTCAGCACGGTGATGGAGAAGCGCGGCCTGGTGTTGTTCGTTGGCGGCACCGGTT
CGGGCAAGTCGACCTCGCTGGCGGCGCTGATCGATTACCGCAACCGCAACAGCGGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCATCGGCACAAGAAATCGATCATCAACCAGCGCGAGGTGGGCGTGGATACACGCAGCTTCCATGC
AGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CACTGGCCTTCGCCGACACCGGGCACCTGGCGATTTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCTGAGGAGCGTCGCCCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCTTTTGTTTCCCAGCG
CCTGGTCAAGACCATTGACGGCAAGCGCCGCGCGGCGGTGGAGGTGATGCTGGGGACGCCGACGGTGCGTGACCTGATCA
AGCGCAACGAATTCTCCGAGCTCAAGGAGATCATGGAGAAGTCGAAGAACCTCGGCATGCAGACCTTCGACCAGGCGTTG
ATCGACCTGGTGCACGACGGCGCCATCGACGAGGAGGAGGCGGTGAAGAACGCCGACTCGGCGAACAACGTGCGCCTGAA
GCTCAAGCTCTACCGCGACAACCCGGCCAATGCCAAGCCCGCGCCCGTCGCAGCGGCTCCGGTGGCGGCACCGGCTGCAG
CGCCGAAGGTTGCCGAGGCGGGCGACTGGGGGCTGGAACTGAAGCTGGAGGACATCGAGGAAGAGCAGCCGCCGGAGGAT
CCGGGGCGCCAGGGTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.125

86.453

0.485
  pilU Vibrio cholerae strain A1552

52.691

86.946

0.458
  pilU Acinetobacter baylyi ADP1

51.705

86.7

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.031

96.552

0.377

Multiple sequence alignment