Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   FIV04_RS13660 Genome accession   NZ_CP045338
Coordinates   3012122..3012715 (-) Length   197 a.a.
NCBI ID   WP_152469384.1    Uniprot ID   A0A5P9BA81
Organism   Vibrio sp. THAF190c     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3007122..3017715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV04_RS13640 (FIV04_13695) aroB 3008053..3009141 (-) 1089 WP_032550007.1 3-dehydroquinate synthase -
  FIV04_RS13645 (FIV04_13700) aroK 3009167..3009685 (-) 519 WP_032550005.1 shikimate kinase AroK -
  FIV04_RS13650 (FIV04_13705) - 3009860..3011497 (-) 1638 Protein_2598 type IV pilus secretin PilQ -
  FIV04_RS13655 (FIV04_13710) pilP 3011617..3012135 (-) 519 WP_152469383.1 pilus assembly protein PilP Machinery gene
  FIV04_RS13660 (FIV04_13715) pilO 3012122..3012715 (-) 594 WP_152469384.1 type 4a pilus biogenesis protein PilO Machinery gene
  FIV04_RS13665 (FIV04_13720) pilN 3012708..3013307 (-) 600 WP_152469385.1 PilN domain-containing protein Machinery gene
  FIV04_RS13670 (FIV04_13725) pilM 3013291..3014316 (-) 1026 WP_152469386.1 type IV pilus assembly protein PilM Machinery gene
  FIV04_RS13675 (FIV04_13730) - 3014464..3017022 (+) 2559 WP_172974250.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22908.23 Da        Isoelectric Point: 4.4977

>NTDB_id=393505 FIV04_RS13660 WP_152469384.1 3012122..3012715(-) (pilO) [Vibrio sp. THAF190c]
MVNFQNLDVDEITEWPLLPQMLVIVLLMLTIQGFGAWFYVMPLNDELEQMKQQEQTLKSTLRIKANKVATLPALQNQLDE
LTSRYDYLLEQLPVQKELASMLASVNELGLDNNLTFTRIDWGAKENKAFLYRLPLNIELTGDYHEIGDFSADIAKLPRII
SFDDVTWQRVSQESSTLHFRVRAYTYQFKAEVKDESR

Nucleotide


Download         Length: 594 bp        

>NTDB_id=393505 FIV04_RS13660 WP_152469384.1 3012122..3012715(-) (pilO) [Vibrio sp. THAF190c]
ATGGTTAACTTTCAAAATCTCGATGTTGACGAAATCACCGAGTGGCCTCTGCTACCACAGATGTTGGTGATTGTTTTATT
GATGCTGACTATTCAAGGTTTCGGCGCTTGGTTCTATGTGATGCCACTCAATGACGAACTAGAGCAAATGAAGCAGCAAG
AGCAGACCTTAAAAAGCACCCTTAGGATCAAAGCCAATAAAGTGGCCACTCTGCCTGCGTTACAGAATCAACTTGATGAA
TTGACCAGTCGCTACGATTACCTACTTGAGCAGCTGCCTGTTCAAAAAGAGTTAGCGAGTATGTTGGCCTCTGTGAATGA
GCTCGGTTTAGACAACAACCTCACTTTTACTCGTATTGACTGGGGGGCGAAAGAGAACAAGGCTTTTCTGTATCGACTGC
CACTGAATATTGAGCTAACGGGTGACTACCATGAGATCGGGGATTTTTCTGCCGACATCGCCAAACTACCGCGCATTATC
AGTTTTGATGATGTGACTTGGCAAAGAGTTAGCCAAGAGAGCAGTACTTTGCATTTCCGTGTTCGTGCATACACCTACCA
ATTCAAGGCAGAGGTCAAAGATGAGTCTCGTTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9BA81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

74.872

98.985

0.741
  pilO Vibrio campbellii strain DS40M4

71.429

95.939

0.685

Multiple sequence alignment