Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FIV04_RS13670 Genome accession   NZ_CP045338
Coordinates   3013291..3014316 (-) Length   341 a.a.
NCBI ID   WP_152469386.1    Uniprot ID   A0A5P9BBX7
Organism   Vibrio sp. THAF190c     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3008291..3019316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV04_RS13645 (FIV04_13700) aroK 3009167..3009685 (-) 519 WP_032550005.1 shikimate kinase AroK -
  FIV04_RS13650 (FIV04_13705) - 3009860..3011497 (-) 1638 Protein_2598 type IV pilus secretin PilQ -
  FIV04_RS13655 (FIV04_13710) pilP 3011617..3012135 (-) 519 WP_152469383.1 pilus assembly protein PilP Machinery gene
  FIV04_RS13660 (FIV04_13715) pilO 3012122..3012715 (-) 594 WP_152469384.1 type 4a pilus biogenesis protein PilO Machinery gene
  FIV04_RS13665 (FIV04_13720) pilN 3012708..3013307 (-) 600 WP_152469385.1 PilN domain-containing protein Machinery gene
  FIV04_RS13670 (FIV04_13725) pilM 3013291..3014316 (-) 1026 WP_152469386.1 type IV pilus assembly protein PilM Machinery gene
  FIV04_RS13675 (FIV04_13730) - 3014464..3017022 (+) 2559 WP_172974250.1 penicillin-binding protein 1A -
  FIV04_RS13680 (FIV04_13735) oxyR 3017107..3017994 (-) 888 WP_152469387.1 DNA-binding transcriptional regulator OxyR -
  FIV04_RS13685 (FIV04_13740) - 3018200..3018928 (+) 729 WP_032549993.1 glutathione peroxidase -

Sequence

Protein


Download         Length: 341 a.a.        Molecular weight: 38182.73 Da        Isoelectric Point: 6.9750

>NTDB_id=393507 FIV04_RS13670 WP_152469386.1 3013291..3014316(-) (pilM) [Vibrio sp. THAF190c]
MGSSLVTGIDINNHSIKAVVLKPVGELYALVGYKELSITDDIFTDNHTLEYQKTVKKLKELRKGLPFGCRHAAISVPDNT
VISKVLQIESDLEDREKEFSIYQTFAHQSPFPIEELSLDFIKLDDKLFGNSSTTSYQVYATRKEVVESRSQALTKAGFRP
IMVDTQAHGLLYIWQLATRLYPEKQSWIMIDIGVNQISLGLIPQSGIPFFKDIAFGTQALYQAGYIEEHTTPFASAEQTQ
AFTVNLIEKLKRQFQLYASANSLQALKGIWLMGEGAETPMLAEQLERHFQLACETLNPLALFENKASNKRYKSMDRHAFG
IAAGIAVSGVKWQGVKHVASN

Nucleotide


Download         Length: 1026 bp        

>NTDB_id=393507 FIV04_RS13670 WP_152469386.1 3013291..3014316(-) (pilM) [Vibrio sp. THAF190c]
ATGGGTTCGTCATTAGTTACAGGTATAGATATCAACAATCACAGCATCAAAGCTGTGGTTTTAAAGCCCGTTGGAGAGTT
ATATGCCTTAGTGGGGTACAAAGAACTGTCTATTACGGACGACATTTTCACAGATAACCACACCTTGGAGTATCAGAAAA
CTGTAAAGAAACTCAAAGAACTTAGGAAAGGCTTACCATTTGGCTGCCGTCATGCCGCTATTTCAGTCCCAGATAATACC
GTGATTAGCAAAGTATTGCAGATAGAAAGTGACCTAGAAGACCGAGAAAAAGAGTTCTCTATTTATCAAACTTTTGCTCA
CCAATCCCCTTTTCCTATTGAGGAGCTGAGCCTAGATTTCATTAAACTGGACGATAAGCTATTTGGAAATAGCTCAACCA
CCAGTTACCAAGTTTATGCAACGCGCAAAGAAGTGGTGGAGAGTCGCTCCCAAGCGCTGACGAAAGCTGGTTTTCGTCCG
ATCATGGTAGATACCCAAGCTCATGGATTACTTTACATTTGGCAGTTGGCAACTCGGCTTTATCCAGAGAAGCAGAGCTG
GATAATGATCGATATAGGGGTCAATCAAATTTCGCTTGGCCTCATTCCGCAAAGCGGGATTCCATTTTTCAAAGATATTG
CCTTTGGTACTCAAGCGCTTTATCAGGCGGGCTATATTGAGGAACACACGACTCCCTTTGCCTCTGCAGAGCAGACTCAA
GCCTTTACCGTGAACTTGATCGAAAAGCTCAAACGCCAGTTTCAGCTCTACGCATCGGCCAACTCTCTTCAAGCACTGAA
GGGAATCTGGTTGATGGGAGAGGGAGCGGAAACCCCGATGCTTGCAGAGCAGTTGGAACGCCATTTTCAGCTCGCGTGTG
AAACGCTTAATCCATTAGCGCTCTTTGAGAATAAAGCCTCTAACAAACGTTATAAGAGCATGGACCGTCATGCCTTTGGT
ATTGCGGCTGGCATTGCAGTGAGTGGTGTGAAGTGGCAAGGAGTGAAGCATGTTGCATCAAATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-327)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5P9BBX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

52.059

99.707

0.519
  pilM Vibrio campbellii strain DS40M4

47.989

100

0.49

Multiple sequence alignment