Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GCS73_RS04895 Genome accession   NZ_CP045308
Coordinates   1007731..1009836 (+) Length   701 a.a.
NCBI ID   WP_012978911.1    Uniprot ID   -
Organism   Legionella longbeachae strain B41211CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1002731..1014836
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCS73_RS04870 (GCS73_04825) - 1003324..1004754 (-) 1431 WP_003632475.1 hypothetical protein -
  GCS73_RS04875 (GCS73_04830) pilM 1004991..1006055 (+) 1065 WP_003632474.1 type IV pilus assembly protein PilM Machinery gene
  GCS73_RS04880 (GCS73_04835) pilN 1006060..1006623 (+) 564 WP_003632473.1 PilN domain-containing protein Machinery gene
  GCS73_RS04885 (GCS73_04840) pilO 1006620..1007219 (+) 600 WP_012978910.1 type 4a pilus biogenesis protein PilO Machinery gene
  GCS73_RS04890 (GCS73_04845) pilP 1007216..1007722 (+) 507 WP_003632471.1 pilus assembly protein PilP Machinery gene
  GCS73_RS04895 (GCS73_04850) pilQ 1007731..1009836 (+) 2106 WP_012978911.1 type IV pilus secretin PilQ Machinery gene
  GCS73_RS04900 (GCS73_04855) aroK 1010172..1010699 (+) 528 WP_003632469.1 shikimate kinase AroK -
  GCS73_RS04905 (GCS73_04860) aroB 1010686..1011789 (+) 1104 WP_003632468.1 3-dehydroquinate synthase -
  GCS73_RS04910 (GCS73_04865) - 1011792..1013225 (+) 1434 WP_012978912.1 SPOR domain-containing protein -
  GCS73_RS04915 (GCS73_04870) - 1014114..1014371 (+) 258 WP_237702682.1 hypothetical protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 77983.04 Da        Isoelectric Point: 9.5391

>NTDB_id=393343 GCS73_RS04895 WP_012978911.1 1007731..1009836(+) (pilQ) [Legionella longbeachae strain B41211CHC]
MQRIVVFLILIGMSLGLAFAQNNSLISVKVIPLPEEKLRIDFIFAYPVKKQPGNFVIENPARIVIDFVDTNLQLPTEQKT
KEIKFGSLASYTLVTVGARVRAVLDLKYAVPYLGSVSGKVYTIILNGKSNELFQAPKEMLITNRPVKTSYEITHFDFRGV
ERQAGRAVVDVSSASIPIDVEEHDKEIIVKFLNTKVPQNLRKRFDVSDFRSPVNLITLQQDGKNARMTLMGDRYFGHNIY
QINKQFMVDVFPLTQEEIEQEKLKKQVFSGRRISLNFQNISIRSVLQLLADFTGINIVASDRVQGNITLRLNNIPWDQAL
DIILKTQGLAQRQNGNVMVIDTQKSFNDMEEQQLKSQHKIQRLEPVRSELIQINYAKATDLAILIKDRQNSLLSDKGKVS
VDTRTNTIWIQDSGSRVDEVRKLIKELDVPVKQVLIEARIVEVTKDFAQDLGIRWGVSKPQHLSGTLAGANQLAQGVAPA
DVTPFTDRLNLDLVAAPLTKANPATVGIALAQLGDNILLDLELSALESEGLAELISSPRLITANQQPALIDSGQEIPYQQ
STSSGATAVAFKKAVLSLKVIPQITPDNKILMDLKINQDLALPNQTYNGVPAIATKEIQTNVLVSNGQTIVLGGIYQQDK
SKTITRVPFFGQLPVVGNLFKNTQIGVHNDELLIFITPKIITNSLSITTIEGRNPVRFDEK

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=393343 GCS73_RS04895 WP_012978911.1 1007731..1009836(+) (pilQ) [Legionella longbeachae strain B41211CHC]
GTGCAAAGAATAGTTGTTTTTTTAATCTTGATAGGAATGAGCTTGGGCTTGGCTTTTGCTCAGAATAACTCTTTGATATC
CGTAAAGGTGATTCCTTTGCCGGAGGAGAAGTTACGTATCGACTTTATATTTGCTTATCCGGTTAAAAAACAACCAGGTA
ATTTTGTAATCGAAAATCCGGCACGCATTGTTATAGATTTTGTTGACACTAATTTACAGTTACCCACTGAGCAGAAAACG
AAGGAAATTAAATTTGGTTCTCTGGCTAGCTATACTCTTGTAACTGTTGGTGCCCGTGTTCGAGCTGTTTTGGATTTAAA
ATACGCTGTGCCATATTTAGGCTCAGTTTCAGGCAAGGTTTATACGATTATTTTAAATGGTAAGAGCAATGAATTATTTC
AAGCCCCTAAAGAAATGTTGATTACCAATCGTCCAGTAAAAACAAGTTATGAAATTACCCATTTTGATTTTCGAGGAGTT
GAACGCCAGGCAGGACGTGCGGTTGTTGATGTTTCGAGCGCCAGTATTCCAATTGATGTAGAAGAGCATGACAAGGAAAT
TATAGTTAAATTTTTAAATACTAAAGTGCCGCAAAATTTAAGGAAACGTTTTGATGTTTCTGATTTTCGCAGTCCGGTGA
ATTTGATTACCTTGCAACAGGATGGAAAAAATGCGCGTATGACCTTAATGGGCGATAGATATTTTGGTCACAATATCTAT
CAGATTAACAAACAGTTTATGGTTGATGTATTTCCGTTGACTCAGGAAGAAATTGAACAGGAAAAACTTAAGAAACAGGT
ATTTTCTGGAAGACGGATTTCTTTAAATTTCCAAAATATCAGCATACGATCAGTATTACAGTTACTTGCTGATTTCACAG
GGATAAATATTGTTGCAAGTGACAGAGTTCAAGGGAATATTACTTTACGGTTAAATAATATTCCCTGGGATCAAGCATTA
GATATTATTTTAAAAACCCAAGGCCTGGCACAGCGCCAAAATGGAAATGTAATGGTGATCGACACGCAAAAATCGTTTAA
TGACATGGAGGAGCAGCAACTTAAGAGCCAACATAAAATCCAAAGACTGGAACCCGTACGTTCCGAACTCATACAAATTA
ATTATGCCAAAGCGACCGATCTCGCAATTTTAATTAAAGACAGACAAAACTCACTTTTATCCGACAAAGGTAAAGTCAGT
GTCGATACACGTACGAATACTATATGGATCCAAGATAGTGGTAGTAGAGTAGATGAGGTCAGAAAGTTAATAAAGGAATT
GGATGTTCCTGTAAAACAGGTACTGATTGAAGCCCGTATTGTTGAGGTGACTAAAGATTTTGCCCAGGATTTAGGTATAC
GTTGGGGGGTTTCCAAGCCGCAACATTTAAGCGGTACTCTAGCTGGGGCAAATCAATTGGCGCAAGGGGTTGCTCCTGCA
GATGTTACACCTTTTACGGACAGATTGAATTTGGATTTGGTAGCAGCCCCTTTGACAAAGGCAAATCCAGCTACAGTAGG
AATTGCTTTAGCGCAGCTTGGCGACAATATATTGCTTGATTTGGAGCTTTCAGCTTTAGAAAGTGAAGGACTCGCAGAGC
TAATTTCCAGCCCCAGGTTAATTACAGCAAACCAGCAGCCTGCATTAATTGACTCAGGCCAAGAAATTCCTTATCAACAA
TCAACTTCTAGTGGTGCAACTGCAGTAGCATTTAAAAAGGCAGTCTTGAGTTTAAAAGTAATACCACAAATTACTCCTGA
TAACAAAATCCTTATGGATTTAAAGATTAATCAGGATCTTGCATTACCTAATCAGACTTATAATGGGGTTCCAGCAATTG
CAACTAAAGAAATTCAGACCAATGTTTTAGTGAGCAATGGACAAACTATTGTATTGGGAGGGATTTATCAACAAGATAAA
AGCAAGACAATTACTCGCGTGCCTTTTTTTGGCCAATTGCCGGTAGTAGGGAATTTATTTAAAAATACACAAATTGGTGT
TCACAATGATGAGCTACTTATTTTTATAACTCCTAAAATAATAACGAATTCATTATCTATCACTACTATTGAGGGGCGAA
ATCCAGTACGTTTTGATGAAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(296-340)

AMIN domain (AMIN)

(41-112)

Bacterial type II/III secretion system short domain (Secretin_N)

(369-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

70.31

96.576

0.679
  pilQ Legionella pneumophila strain ERS1305867

70.37

96.291

0.678
  pilQ Pseudomonas aeruginosa PAK

36.298

99.429

0.361

Multiple sequence alignment