Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   GCB94_RS05635 Genome accession   NZ_CP045306
Coordinates   1013645..1015750 (+) Length   701 a.a.
NCBI ID   WP_003632470.1    Uniprot ID   -
Organism   Legionella longbeachae strain B1445CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1008645..1020750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCB94_RS05610 (GCB94_05590) - 1009242..1010672 (-) 1431 WP_003632475.1 hypothetical protein -
  GCB94_RS05615 (GCB94_05595) pilM 1010909..1011969 (+) 1061 Protein_886 type IV pilus assembly protein PilM -
  GCB94_RS05620 (GCB94_05600) pilN 1011974..1012537 (+) 564 WP_003632473.1 PilN domain-containing protein Machinery gene
  GCB94_RS05625 (GCB94_05605) pilO 1012534..1013133 (+) 600 WP_003632472.1 type 4a pilus biogenesis protein PilO Machinery gene
  GCB94_RS05630 (GCB94_05610) pilP 1013130..1013636 (+) 507 WP_003632471.1 pilus assembly protein PilP Machinery gene
  GCB94_RS05635 (GCB94_05615) pilQ 1013645..1015750 (+) 2106 WP_003632470.1 type IV pilus secretin PilQ Machinery gene
  GCB94_RS05640 (GCB94_05620) aroK 1016092..1016613 (+) 522 WP_027270601.1 shikimate kinase AroK -
  GCB94_RS05645 (GCB94_05625) aroB 1016600..1017703 (+) 1104 WP_003632468.1 3-dehydroquinate synthase -
  GCB94_RS05650 (GCB94_05630) - 1017706..1019139 (+) 1434 WP_003632467.1 SPOR domain-containing protein -
  GCB94_RS05655 (GCB94_05635) - 1019956..1020285 (+) 330 WP_003632466.1 hypothetical protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 77957.01 Da        Isoelectric Point: 9.5611

>NTDB_id=393306 GCB94_RS05635 WP_003632470.1 1013645..1015750(+) (pilQ) [Legionella longbeachae strain B1445CHC]
MQRIVVFLILIGMSLGLAFAQNNSLISVKVIPLPEEKLRIDFIFAHPVKKQPGNFVIENPARIVIDFVDTNLQLPTEQKT
KEIKFGSLASYTLVTVGARVRAVLDLKYAVPYLGSVSGKVYTIILNGKSNELFQAPKEMLITNRPVKTSYEITHFDFRGV
ERQAGRAVVDVSSASIPIDVEEHDKEIIVKFLNTKVPQNLRKRFDVSDFRSPVNLITLQQDGKNARMTLMGDRYFGHNIY
QINKQFMVDVFPLTQEEIEQEKLKKQVFSGRRISLNFQNISIRSVLQLLADFTGINIVASDRVQGNITLRLNNIPWDQAL
DIILKTQGLAQRQNGNVMVIDTQKSFNDMEEQQLKSQHKIQRLEPVRSELIQINYAKATDLAILIKDRQNSLLSDKGKVS
VDTRTNTIWIQDSGSRVDEVRKLIKELDVPVKQVLIEARIVEVTKDFAQDLGIRWGVSKPQHLSGTLAGANQLAQGVAPA
DVTPFTDRLNLDLVAAPLTKANPATVGIALAQLGDNILLDLELSALESEGLAELISSPRLITANQQPALIDSGQEIPYQQ
STSSGATAVAFKKAVLSLKVIPQITPDNKILMDLKINQDLALPNQTYNGVPAIATKEIQTNVLVSNGQTIVLGGIYQQDK
SKTITRVPFFGQLPVVGNLFKNTQIGVHNDELLIFITPKIITNSLSITTIEGRNPVRFDEK

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=393306 GCB94_RS05635 WP_003632470.1 1013645..1015750(+) (pilQ) [Legionella longbeachae strain B1445CHC]
GTGCAAAGAATAGTTGTTTTTTTAATCTTGATAGGAATGAGCTTGGGCTTGGCTTTTGCTCAGAATAACTCTTTGATATC
CGTAAAGGTGATTCCTTTGCCGGAGGAGAAGTTACGTATCGACTTTATATTTGCTCATCCGGTTAAAAAACAACCAGGTA
ATTTTGTAATCGAAAATCCGGCACGCATTGTTATAGATTTTGTTGACACTAATTTACAGTTACCCACTGAGCAGAAAACG
AAGGAAATTAAATTTGGTTCTCTGGCTAGCTATACTCTTGTAACTGTTGGTGCCCGTGTTCGAGCTGTTTTGGATTTAAA
ATACGCTGTGCCATATTTAGGCTCAGTTTCAGGCAAGGTTTATACGATTATTTTAAATGGTAAGAGCAATGAATTATTTC
AAGCCCCTAAAGAAATGTTGATTACCAATCGTCCAGTAAAAACAAGTTATGAAATTACCCATTTTGATTTTCGAGGAGTT
GAACGCCAGGCAGGACGTGCGGTTGTTGATGTTTCGAGCGCCAGTATTCCAATTGATGTAGAAGAGCATGACAAGGAAAT
TATAGTTAAATTTTTAAATACTAAAGTGCCGCAAAATTTAAGGAAACGTTTTGATGTTTCTGATTTTCGCAGTCCGGTGA
ATTTGATTACCTTGCAACAGGATGGAAAAAATGCGCGTATGACCTTAATGGGCGATAGATATTTTGGTCACAATATCTAT
CAGATTAACAAACAGTTTATGGTTGATGTATTTCCGTTGACTCAGGAAGAAATTGAACAGGAAAAACTTAAGAAACAGGT
ATTTTCTGGAAGACGGATTTCTTTAAATTTCCAAAATATCAGCATACGATCAGTATTACAGTTACTTGCTGATTTCACAG
GGATAAATATTGTTGCAAGTGACAGAGTTCAAGGGAATATTACTTTACGGTTAAATAATATTCCCTGGGATCAAGCATTA
GATATTATTTTAAAAACCCAAGGCCTGGCACAGCGCCAAAATGGAAATGTAATGGTGATCGACACGCAAAAATCGTTTAA
TGACATGGAGGAGCAGCAACTTAAGAGCCAACATAAAATCCAAAGACTGGAACCCGTACGTTCCGAACTCATACAAATTA
ATTATGCCAAAGCGACCGATCTCGCAATTTTAATTAAAGACAGACAAAACTCACTTTTATCCGACAAAGGTAAAGTCAGT
GTCGATACACGTACGAATACTATATGGATCCAAGATAGTGGTAGTAGAGTAGATGAGGTCAGAAAGTTAATAAAGGAATT
GGATGTTCCTGTAAAACAGGTACTGATTGAAGCCCGTATTGTTGAGGTGACTAAAGATTTTGCCCAGGATTTAGGTATAC
GTTGGGGGGTTTCCAAGCCGCAACATTTAAGCGGTACTCTAGCTGGGGCAAATCAATTGGCGCAAGGGGTTGCTCCTGCA
GATGTTACACCTTTTACGGACAGATTGAATTTGGATTTGGTAGCAGCCCCTTTGACAAAGGCAAATCCAGCTACAGTAGG
AATTGCTTTAGCGCAGCTTGGCGACAATATATTGCTTGATTTGGAGCTTTCAGCTTTAGAAAGTGAAGGACTCGCAGAGC
TAATTTCCAGCCCCAGGTTAATTACAGCAAACCAGCAGCCTGCATTAATTGACTCAGGCCAAGAAATTCCTTATCAACAA
TCAACTTCTAGTGGTGCAACTGCAGTAGCATTTAAAAAGGCAGTCTTGAGTTTAAAAGTAATACCACAAATTACTCCTGA
TAACAAAATCCTTATGGATTTAAAGATTAATCAGGATCTTGCATTACCTAATCAGACTTATAATGGGGTTCCAGCAATTG
CAACTAAAGAAATTCAGACCAATGTTTTAGTGAGCAATGGACAAACTATTGTATTGGGAGGGATTTATCAACAAGATAAA
AGCAAGACAATTACTCGCGTGCCTTTTTTTGGCCAATTGCCGGTAGTAGGGAATTTATTTAAAAATACACAAATTGGTGT
TCACAATGATGAGCTACTTATTTTTATAACTCCTAAAATAATAACGAATTCATTATCTATCACTACTATTGAGGGGCGAA
ATCCAGTACGTTTTGATGAAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-681)

Secretin and TonB N terminus short domain (STN)

(296-340)

AMIN domain (AMIN)

(41-112)

Bacterial type II/III secretion system short domain (Secretin_N)

(369-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

70.31

96.576

0.679
  pilQ Legionella pneumophila strain ERS1305867

70.37

96.291

0.678
  pilQ Pseudomonas aeruginosa PAK

36.298

99.429

0.361

Multiple sequence alignment