Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   GB851_RS02725 Genome accession   NZ_CP045141
Coordinates   529564..531249 (+) Length   561 a.a.
NCBI ID   WP_003787777.1    Uniprot ID   F5S8J8
Organism   Kingella kingae strain F41215CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 524564..536249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS02700 (GB851_02800) - 524965..526590 (+) 1626 WP_164538084.1 ABC-F family ATPase -
  GB851_RS02705 (GB851_02805) pgeF 526643..527422 (-) 780 WP_223877603.1 peptidoglycan editing factor PgeF -
  GB851_RS02710 (GB851_02810) cyaY 527572..527895 (-) 324 WP_099046165.1 iron donor protein CyaY -
  GB851_RS02715 (GB851_02815) - 527986..528180 (+) 195 WP_003790918.1 lipoprotein -
  GB851_RS02720 (GB851_02820) lysA 528149..529378 (+) 1230 WP_134793864.1 diaminopimelate decarboxylase -
  GB851_RS02725 (GB851_02825) pilF 529564..531249 (+) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GB851_RS02730 (GB851_02830) pilG 531296..532564 (+) 1269 WP_003790915.1 type II secretion system F family protein Machinery gene
  GB851_RS02735 (GB851_02835) pilD 532636..533475 (+) 840 WP_338153836.1 A24 family peptidase Machinery gene
  GB851_RS02740 (GB851_02840) coaE 533477..534085 (+) 609 WP_003787784.1 dephospho-CoA kinase -
  GB851_RS02745 (GB851_02850) zapD 534200..534961 (+) 762 WP_003790912.1 cell division protein ZapD -
  GB851_RS02750 (GB851_02855) pncC 534981..535457 (+) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  GB851_RS02755 (GB851_02860) - 535467..536087 (+) 621 WP_164538085.1 L-threonylcarbamoyladenylate synthase -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62226.13 Da        Isoelectric Point: 5.1186

>NTDB_id=392275 GB851_RS02725 WP_003787777.1 529564..531249(+) (pilF) [Kingella kingae strain F41215CHC]
MSAGLLRILVQKKLVPQENLAKYQEAIKADKPILASIFADKLITPEDLAELCATMFNYPMLDLNFFPRSKIVPDILDESQ
MMELRCIPLFKRGKKLYLAVSDPTNIQNLQKVVFSSGLAIDLVIVRDDQLNTVLEWFGQSNSSLLKEMGMDSSLQEAQAQ
TGMIVDGEEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGQLREIVQPPLALRSQLASRIKVMAKLDIS
EKRVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKKLIMDAIYRPYGMVLVTGPT
GSGKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIK
AAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPENVLRDAGFTDEDLAKDW
TMYRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLCDLRRSGLLKVMQGVTTLEEVIATTN
E

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=392275 GB851_RS02725 WP_003787777.1 529564..531249(+) (pilF) [Kingella kingae strain F41215CHC]
ATGAGCGCAGGTTTATTGCGAATTTTGGTACAGAAAAAACTCGTACCACAAGAGAATTTAGCCAAATATCAAGAAGCGAT
TAAAGCCGATAAACCGATTTTGGCTTCCATTTTTGCTGACAAGCTGATTACACCAGAAGACTTGGCAGAGCTGTGTGCTA
CCATGTTTAACTATCCGATGTTGGATTTAAACTTCTTCCCACGCAGCAAAATCGTCCCTGATATTTTGGATGAATCGCAA
ATGATGGAATTGCGCTGCATTCCATTGTTTAAGCGTGGTAAAAAATTGTATTTGGCGGTGTCTGACCCAACCAATATCCA
AAATTTGCAAAAAGTCGTGTTCAGCAGCGGCTTGGCGATTGATTTAGTGATTGTGCGCGATGATCAATTAAATACCGTGT
TGGAATGGTTTGGTCAAAGCAATAGCAGCCTGCTTAAAGAAATGGGCATGGACAGCAGCCTGCAAGAAGCACAAGCCCAA
ACAGGCATGATTGTGGATGGCGAAGAAGAAGAGGACGGCCCTGTTGCCCGTTTCATTCAAAAAATTCTGCACGATGCCGT
GACATCGGGTGCGTCCGATATTCACTTTGAGTTCTATGAATTTATGGCGCGTGTGCGTTTCCGTACAGACGGTCAGTTGC
GCGAAATCGTACAGCCACCATTGGCATTGCGTAGCCAGCTGGCATCGCGCATCAAGGTAATGGCGAAACTGGATATTTCT
GAAAAGCGCGTCCCACAAGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACAC
CATGCCGTGTTTGTTTGGCGAAAAAGTCGTGATGCGTATTTTGAACTCCGATGCCGCCAGCTTGAACATTGACCAACTGG
GTTTTGAAGACTTCCAGAAAAAATTGATTATGGACGCGATTTATCGTCCATACGGCATGGTGCTGGTAACAGGGCCTACG
GGCTCGGGTAAAACCGTATCGCTTTACACTTGCTTAAACATTTTGAATACCGATGACGTAAACATCTCCACCGCGGAAGA
CCCTGCGGAGATTAACTTACCAGGCATCAATCAAGTAAACGTAAACGACAAACAAGGCTTAACTTTTGAAGCTGCGCTAA
AAGCATTCTTGCGTCAAGACCCAGATATCATCATGATTGGTGAGATTCGTGACTTGGGTACAGCCGATATTGCGATTAAA
GCCGCACAAACAGGTCACATGGTGTTCTCAACCTTGCACACCAACAATGCCCCAGCCACCTTGTCGCGTATGTTGAACAT
GGGTGTTGCGCCGTTTAATATTGCATCGGCAGTGAACTTGATTATGGCGCAGCGTTTGGCACGTCGTTTGTGCAGCGCGT
GTAAAGCCCCAATGGAACGTCCGCCTGAAAATGTATTGCGCGATGCAGGTTTTACTGACGAAGATTTGGCAAAAGATTGG
ACAATGTATCGTGCCGTAGGTTGTGATGCGTGTAAAGGCAAAGGCTACAAAGGTCGTGCAGGCTTGTACGAAGTGATGCC
GATGACGGAAAAAATGAAAGCCGTTATCATGAAAGGCGGTACAGAAGTGGATATTGCTAACATTGCCTATGAAGAAGGTT
TGTGCGATTTGCGCCGTTCTGGCTTGCTCAAAGTGATGCAAGGTGTAACCACTTTGGAAGAAGTGATTGCGACAACCAAC
GAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(181-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F5S8J8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.271

100

0.683
  pilB Acinetobacter baumannii D1279779

52.91

100

0.535
  pilB Acinetobacter baylyi ADP1

51.865

100

0.52
  pilB Legionella pneumophila strain ERS1305867

47.695

100

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.455
  pilB Vibrio campbellii strain DS40M4

45.421

95.365

0.433
  pilB Vibrio cholerae strain A1552

46.318

91.979

0.426
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

92.692

0.369

Multiple sequence alignment