Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   GB851_RS02735 Genome accession   NZ_CP045141
Coordinates   532636..533475 (+) Length   279 a.a.
NCBI ID   WP_338153836.1    Uniprot ID   -
Organism   Kingella kingae strain F41215CHC     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527636..538475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GB851_RS02715 (GB851_02815) - 527986..528180 (+) 195 WP_003790918.1 lipoprotein -
  GB851_RS02720 (GB851_02820) lysA 528149..529378 (+) 1230 WP_134793864.1 diaminopimelate decarboxylase -
  GB851_RS02725 (GB851_02825) pilF 529564..531249 (+) 1686 WP_003787777.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GB851_RS02730 (GB851_02830) pilG 531296..532564 (+) 1269 WP_003790915.1 type II secretion system F family protein Machinery gene
  GB851_RS02735 (GB851_02835) pilD 532636..533475 (+) 840 WP_338153836.1 A24 family peptidase Machinery gene
  GB851_RS02740 (GB851_02840) coaE 533477..534085 (+) 609 WP_003787784.1 dephospho-CoA kinase -
  GB851_RS02745 (GB851_02850) zapD 534200..534961 (+) 762 WP_003790912.1 cell division protein ZapD -
  GB851_RS02750 (GB851_02855) pncC 534981..535457 (+) 477 WP_003787789.1 nicotinamide-nucleotide amidase -
  GB851_RS02755 (GB851_02860) - 535467..536087 (+) 621 WP_164538085.1 L-threonylcarbamoyladenylate synthase -
  GB851_RS02760 (GB851_02865) - 536164..537357 (-) 1194 WP_164538086.1 acetate kinase -

Sequence

Protein


Download         Length: 279 a.a.        Molecular weight: 31026.80 Da        Isoelectric Point: 8.1379

>NTDB_id=392277 GB851_RS02735 WP_338153836.1 532636..533475(+) (pilD) [Kingella kingae strain F41215CHC]
MVIYRLPVMLNRDWTEAACEQLNINDEFWQKQPASVEQGLREHFGLNRAVSGTFNLMTPPSRCGNCGSAVRPWQNIPVLS
WLMLRGKCAGCHKPISIRYPLVELLTGVLFAVMAFKYGWTWYTVGACLFTAYVVAMTFIDADTQLLPDDLTIPLMRWGLL
FNLLTGFIPLTQSVWGMLIGYMSLWTLSQTYKLIRGVDGMGAGDFKMLAAIGAWLGAWILPVVIFAAAMVFLVVAIIRGI
GKEQPMAYGPCLGVAGWLVFLFYDVVMRGVTAWLTGSGF

Nucleotide


Download         Length: 840 bp        

>NTDB_id=392277 GB851_RS02735 WP_338153836.1 532636..533475(+) (pilD) [Kingella kingae strain F41215CHC]
GTGGTCATTTATCGGCTGCCCGTGATGCTCAATCGCGATTGGACAGAAGCAGCCTGCGAGCAACTCAATATCAACGATGA
ATTTTGGCAAAAGCAGCCTGCATCCGTAGAACAAGGTCTGCGCGAACACTTTGGCTTAAACCGTGCTGTGTCAGGCACAT
TCAACTTGATGACACCGCCATCGCGTTGTGGCAACTGCGGTTCAGCCGTGCGTCCTTGGCAAAACATTCCTGTGTTGAGC
TGGTTGATGTTGCGTGGCAAATGTGCAGGCTGCCACAAACCCATTAGCATTCGCTATCCTTTGGTAGAACTACTCACAGG
CGTGTTGTTTGCGGTAATGGCATTCAAGTATGGCTGGACTTGGTACACCGTAGGCGCGTGTTTGTTTACCGCTTATGTCG
TTGCCATGACCTTTATTGATGCCGACACCCAACTTTTGCCCGATGACTTAACCATTCCATTGATGCGCTGGGGCTTGTTG
TTTAACTTGCTTACAGGCTTCATTCCGCTAACGCAATCGGTTTGGGGCATGTTGATTGGCTACATGAGTCTGTGGACGCT
GTCGCAAACCTATAAACTAATTCGTGGCGTGGACGGCATGGGCGCAGGCGATTTCAAAATGCTGGCTGCGATTGGTGCGT
GGCTGGGTGCATGGATTTTGCCTGTGGTGATTTTTGCTGCGGCGATGGTGTTTTTGGTGGTTGCCATTATTCGGGGCATT
GGCAAAGAACAGCCAATGGCATACGGCCCGTGTTTGGGCGTTGCCGGCTGGTTGGTCTTTTTGTTTTACGATGTGGTGAT
GCGTGGCGTAACAGCTTGGCTCACTGGGTCGGGTTTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(2-116)

Type IV leader peptidase family (Peptidase_A24)

(129-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

50.182

98.566

0.495
  pilD Vibrio cholerae strain A1552

39.927

97.849

0.391
  pilD Acinetobacter baumannii D1279779

40

96.774

0.387
  pilD Vibrio campbellii strain DS40M4

40.152

94.624

0.38
  pilD Acinetobacter nosocomialis M2

39.259

96.774

0.38

Multiple sequence alignment