Detailed information NTG0392021

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: GA645_RS02030
• Genome accession: NZ_CP045118
• Coordinates: 426326..427471 (+)
• Length: 381 a.a.
• NCBI ID: WP_152219498.1
• Uniprot ID: -
• Organism: Pseudomonas sp. SCB32
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 421326..432471

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GA645_RS02005	-	421475..422068 (-)	594	WP_152219487.1	YggT family protein	-
GA645_RS02010	-	422211..423284 (-)	1074	WP_152219489.1	hypothetical protein	-
GA645_RS02015	proC	423431..424264 (-)	834	WP_152219491.1	pyrroline-5-carboxylate reductase	-
GA645_RS02020	-	424282..424971 (-)	690	WP_152219494.1	YggS family pyridoxal phosphate-dependent enzyme	-
GA645_RS02025	pilT	425044..426078 (+)	1035	WP_152219496.1	type IV pilus twitching motility protein PilT	Machinery gene
GA645_RS02030	pilU	426326..427471 (+)	1146	WP_152219498.1	type IV pilus ATPase PilU	Machinery gene
GA645_RS02035	-	427550..427954 (+)	405	WP_152219500.1	TM2 domain-containing protein	-
GA645_RS02040	-	428273..429646 (+)	1374	WP_152219502.1	cystathionine beta-synthase	-
GA645_RS02045	-	429643..430812 (+)	1170	WP_152219504.1	cystathionine gamma-synthase	-
GA645_RS02050	-	431016..432287 (-)	1272	WP_152219506.1	dihydroorotase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42546.82 Da
• Isoelectric Point: 6.7258

>NTDB_id=392021 GA645_RS02030 WP_152219498.1 426326..427471(+) (pilU) [Pseudomonas sp. SCB32]
MEFEKLLRLMVEKGASDLFITAGVPPSMKVNGKVLPVTKTALSPEQTRETVLSVMNEQQRREFAENHECNFAISARGVGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEVLKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVSTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSTMTQGLTLEMSDDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=392021 GA645_RS02030 WP_152219498.1 426326..427471(+) (pilU) [Pseudomonas sp. SCB32]
ATGGAATTCGAGAAGCTGTTGCGCCTCATGGTGGAAAAGGGTGCCTCCGACCTCTTCATCACGGCGGGCGTGCCGCCTTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCGCGCTGTCGCCGGAGCAGACCCGTGAAACCGTGCTCTCGG
TGATGAACGAGCAGCAGCGCCGCGAGTTCGCCGAGAACCACGAGTGCAACTTCGCCATCAGCGCCCGTGGCGTCGGCCGT
TTCCGTGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGCTGCGCCGCATCGAGACCAACATCCCGACCATCGA
AGACCTCAAGCTGCCGGAAGTCCTCAAGAAGCTGGCGATGACCAAGCGTGGCCTGGTGATCTTCGTCGGTGCCACCGGTA
CCGGTAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGCCACATCATCTCCATCGAAGAC
CCGATCGAATACATCCACCAGCACCAGGGTTGCATCGTCACCCAGCGCGAGGTGGGCTTGGATACCGATTCTTTCGAAGT
GGCCCTGAAGAACACCCTGCGCCAGGCGCCTGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGACCACG
CGGTGGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCGACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCATGGCCAGGTGTGGATGGACCTGTCGCTCAACCTCAAGGCCATCGTCGCCCAGCA
GCTGGTGTCGACCCCGGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAACGCTCCACCGAGCAGGGGATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACAGCCAGGGCGAGATCACCTACGAAGACGCACTGGCGTACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTGGGTTCGGAAACCGATGCCGACCACCTCTCCACCATGACCCAGGGGTTGACCCTGGAGATGAGCGACG
ACGACCCCAATCGCCGATTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.976	100	0.89
pilU	Acinetobacter baylyi ADP1	63.298	98.688	0.625
pilU	Vibrio cholerae strain A1552	59.03	97.375	0.575
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.976	0.383