Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   GBN68_RS08385 Genome accession   NZ_CP045101
Coordinates   1763023..1764216 (-) Length   397 a.a.
NCBI ID   WP_234953187.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ210     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1758023..1769216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBN68_RS08370 (GBN68_08415) - 1760060..1761088 (+) 1029 WP_004979806.1 AraC family transcriptional regulator -
  GBN68_RS08375 (GBN68_08420) gcvH 1761161..1761535 (+) 375 WP_004693240.1 glycine cleavage system protein GcvH -
  GBN68_RS08380 (GBN68_08425) - 1761797..1762975 (+) 1179 WP_234953186.1 MFS transporter -
  GBN68_RS08385 (GBN68_08430) pilU 1763023..1764216 (-) 1194 WP_234953187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  GBN68_RS08390 (GBN68_08435) - 1764456..1765517 (-) 1062 WP_234953188.1 3-deoxy-7-phosphoheptulonate synthase -
  GBN68_RS08395 (GBN68_08440) - 1766009..1768108 (-) 2100 WP_234953189.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 44761.10 Da        Isoelectric Point: 6.5063

>NTDB_id=391912 GBN68_RS08385 WP_234953187.1 1763023..1764216(-) (pilU) [Acinetobacter johnsonii strain AJ210]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAINVPNVSRFRVNVFQQQQHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRSRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANPQLNSNSALSFDQAIAEKAKDAEEEKADA

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=391912 GBN68_RS08385 WP_234953187.1 1763023..1764216(-) (pilU) [Acinetobacter johnsonii strain AJ210]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTCATGAAGCCATTGGGTCAACAAAATTTGC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTGGAGTTCGAAACCGAGCTTGAGTGT
AATTTTGCAATTAACGTTCCCAATGTTTCGCGTTTTCGCGTCAATGTTTTTCAGCAGCAACAGCATGTGGGGATGGTGAT
TCGTACGATTACCGCGGAAATTCCCAACTTCACTAAGTTACAACTTCCAACGTCTTTAAAAGACGTGATTATGGAAAAAC
GTGGTTTGGTCCTCGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTAGCAGCCATGATTGATCATCGAAATGAA
AACTCAGCAGGGCACATTATTACGGTTGAAGATCCTGTGGAGTATGTACATAAACATAAGAAATCGATGATTACTCATCG
AGAAGTGGGTGTGGACTGTCATTCTTGGCACAATGCTTTGAAAAATACTCTGCGCCAAGCACCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACGATGGAGCATGCCATAGCTTTCGCGGAAACAGGTCATTTATGCCTGGGGACATTGCAT
GCCAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCGGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTTTGGTGCGAACTGAAGATGGGCGTAGTCGCCGAGCAGCAGTAGAAA
TCATGCTCAATACACCGTTGATGTCAGACTTGATCCTTAAAGGGAATTTCCACGAACTGAAAGAAGTGATGTCAAAATCA
CGAGAGCTCGGTATGCAGACCTTCGACCAAGCTTTATTTGATTTATACAATCAAGGTGCAATCGCTTATGAAGAGGCGCT
ACGAAATGCAGACTCGGTCAATGAATTGCGTCTACAGATTAAATTAAAGAGTAGTCGTGCCAACCCGCAGTTAAACTCAA
ATAGTGCTTTGTCTTTTGATCAAGCGATTGCTGAAAAAGCCAAAGACGCTGAAGAAGAGAAGGCCGACGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(121-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.774

90.428

0.531
  pilU Acinetobacter baylyi ADP1

55.372

91.436

0.506
  pilU Vibrio cholerae strain A1552

50.289

87.154

0.438
  pilT Legionella pneumophila strain Lp02

42.183

85.39

0.36
  pilT Legionella pneumophila strain ERS1305867

42.183

85.39

0.36

Multiple sequence alignment