Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   F9230_RS08950 Genome accession   NZ_CP045099
Coordinates   1843142..1844335 (+) Length   397 a.a.
NCBI ID   WP_328282646.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain AJ14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1838142..1849335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F9230_RS08940 (F9230_08925) - 1839266..1841365 (+) 2100 WP_168159613.1 EAL domain-containing protein -
  F9230_RS08945 (F9230_08930) - 1841843..1842904 (+) 1062 WP_234950127.1 3-deoxy-7-phosphoheptulonate synthase -
  F9230_RS08950 (F9230_08935) pilU 1843142..1844335 (+) 1194 WP_328282646.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  F9230_RS08955 (F9230_08940) - 1844382..1845560 (-) 1179 WP_234950128.1 MFS transporter -
  F9230_RS08960 (F9230_08945) gcvH 1845820..1846194 (-) 375 WP_004693240.1 glycine cleavage system protein GcvH -
  F9230_RS08965 (F9230_08950) - 1846267..1847295 (-) 1029 WP_087528086.1 AraC family transcriptional regulator -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 44735.10 Da        Isoelectric Point: 6.5064

>NTDB_id=391865 F9230_RS08950 WP_328282646.1 1843142..1844335(+) (pilU) [Acinetobacter johnsonii strain AJ14]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISANFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQLPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAITYEEALRNADSVNELRLQIKLKSSRANPQLNSSSALSFDQAVAEKAKEAEEEKDDA

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=391865 F9230_RS08950 WP_328282646.1 1843142..1844335(+) (pilU) [Acinetobacter johnsonii strain AJ14]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAAACTTTCCGCCAAGTATTAAGCATCAGGGCCTTATGAAGCCATTGGGTCAACAAAACTTGC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTAGAGTTCGAAACTGAGCTGGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTCTCACGTTTTCGCGTCAATGTTTTTCAGCAGCAACTACATGTAGGTATGGTGAT
TCGTACGATTACAGCGGAAATTCCTAACTTTACTAAATTACAACTTCCAACGTCTTTAAAAGACGTGATTATGGAAAAAC
GTGGTTTGGTACTTGTTGTCGGTGGTACAGGGTCGGGTAAATCGACGTCTTTGGCAGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGATCCTGTGGAGTATGTACATAAACATAAGAAATCGATGATTACTCATCG
AGAAGTGGGTGTGGACTGTCACTCTTGGCACAATGCTTTGAAAAATACTTTGCGCCAAGCGCCCGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACTATGGAGCATGCCATAGCTTTTGCAGAAACAGGCCACTTATGCCTAGGCACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTTCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCTAATATGAAGGCGATAATTTCACAGCGTCTGGTGCGAACTGAAGATGGGCGAGGTCGCCGAGCAGCAGTGGAAA
TCATGCTCAATACGCCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCATGAACTCAAAGAAGTGATGTCGAAGTCA
CGAGAGCTCGGTATGCAGACCTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATTACTTACGAAGAAGCGCT
ACGAAATGCAGATTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCTAACCCACAGTTAAACTCAA
GTAGTGCTTTGTCTTTTGATCAAGCGGTTGCCGAAAAGGCCAAAGAAGCTGAAGAAGAGAAAGACGACGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.61

90.428

0.539
  pilU Acinetobacter baylyi ADP1

54.821

91.436

0.501
  pilU Vibrio cholerae strain A1552

50.578

87.154

0.441
  pilT Acinetobacter baylyi ADP1

43.243

83.879

0.363

Multiple sequence alignment