Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PSM_RS12635 Genome accession   NC_014803
Coordinates   2752652..2753788 (-) Length   378 a.a.
NCBI ID   WP_013465850.1    Uniprot ID   A0AA37S1K5
Organism   Pseudoalteromonas sp. SM9913     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2747652..2758788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSM_RS12605 (PSM_A2574) - 2748169..2749062 (+) 894 WP_013465844.1 hypothetical protein -
  PSM_RS12610 (PSM_A2575) - 2749140..2749613 (+) 474 WP_013465845.1 hypothetical protein -
  PSM_RS12615 (PSM_A2576) rsmE 2749684..2750418 (+) 735 WP_013465846.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PSM_RS12620 (PSM_A2577) gshB 2750485..2751435 (+) 951 WP_013465847.1 glutathione synthase -
  PSM_RS12625 (PSM_A2578) - 2751571..2752128 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  PSM_RS12630 (PSM_A2579) ruvX 2752146..2752595 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  PSM_RS12635 (PSM_A2580) pilU 2752652..2753788 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSM_RS12640 (PSM_A2581) pilT 2753798..2754838 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  PSM_RS12645 (PSM_A2582) - 2754841..2755554 (+) 714 WP_013465852.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSM_RS12650 (PSM_A2583) proC 2755572..2756393 (+) 822 WP_013465853.1 pyrroline-5-carboxylate reductase -
  PSM_RS12655 (PSM_A2584) - 2756420..2756953 (+) 534 WP_008113334.1 YggT family protein -
  PSM_RS12660 (PSM_A2585) - 2757012..2757455 (+) 444 WP_013465854.1 DUF4426 domain-containing protein -
  PSM_RS12665 (PSM_A2586) - 2757458..2758057 (+) 600 WP_016900586.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41994.04 Da        Isoelectric Point: 6.2895

>NTDB_id=39157 PSM_RS12635 WP_013465850.1 2752652..2753788(-) (pilU) [Pseudoalteromonas sp. SM9913]
MTLSLNHFLQIMIDKKGSDLFVSSQLPISAKINGELTPLNDDKLTDEQALELVESAMSEKQKAEFHNTKECNFAIATSEG
RFRISAFWQRDRAGMVIRRIVTKIPDVKELGLPSALTDVIMSKRGLVLFVGGTGTGKSTSLAALIGYRNRNQRGHILTIE
DPIEFVHEHQKSIITQREVGLDTESFESALKSSLRQAPDVILIGEIRSQETMEYALSFAETGHLCVATLHANNANQAIDR
IMHLVPKEKHDKLKYDLALNLRAIIAQQLIPTSDGEGRVAAIEVLLNTPMVSELIKNGNIGGIKETMAKSTEMGMQTFDQ
ALFELYRQQRINYADALHHADSPNDLRLMIKLRNNEQQGAGFLQGVTIDGLDDKGNMT

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=39157 PSM_RS12635 WP_013465850.1 2752652..2753788(-) (pilU) [Pseudoalteromonas sp. SM9913]
ATGACTTTATCTTTAAATCACTTTTTACAAATAATGATCGACAAAAAAGGCTCTGATTTATTTGTATCGAGCCAACTTCC
TATTAGCGCTAAAATTAACGGCGAGCTAACTCCTCTTAATGACGATAAATTAACAGATGAGCAAGCGCTAGAGTTAGTCG
AATCAGCGATGAGTGAAAAACAAAAAGCAGAGTTTCATAACACTAAAGAATGTAACTTTGCGATTGCAACTAGCGAAGGG
CGCTTTCGTATTTCGGCTTTTTGGCAACGCGACAGAGCGGGCATGGTTATTCGTCGAATTGTCACTAAAATACCCGATGT
TAAAGAGCTTGGATTACCCTCTGCATTGACCGATGTGATTATGTCAAAGCGCGGACTCGTGTTATTTGTAGGTGGTACGG
GAACCGGTAAATCAACGTCTTTGGCGGCACTTATTGGTTATAGAAATCGCAATCAACGTGGGCATATTTTAACCATAGAA
GATCCGATTGAATTTGTGCATGAACATCAAAAAAGTATTATTACCCAACGTGAAGTGGGTTTAGATACTGAAAGCTTTGA
ATCGGCACTTAAAAGCTCTTTGCGCCAAGCGCCTGATGTTATTTTAATTGGTGAAATTCGTTCGCAAGAAACCATGGAAT
ATGCGTTGAGCTTTGCAGAAACGGGTCATTTATGTGTTGCCACCTTGCATGCTAACAATGCTAATCAAGCTATTGACCGC
ATTATGCACTTAGTACCAAAAGAAAAGCACGATAAGCTTAAATATGACTTAGCATTAAATTTACGCGCTATCATTGCTCA
GCAACTTATTCCTACCTCTGATGGGGAAGGGCGTGTTGCTGCCATTGAGGTATTACTTAATACGCCAATGGTCAGTGAGC
TAATTAAAAACGGTAATATTGGTGGCATTAAAGAAACCATGGCTAAATCAACAGAGATGGGCATGCAAACCTTTGACCAA
GCGCTGTTTGAGCTTTACAGACAGCAACGTATTAATTATGCCGATGCGCTGCATCATGCGGATTCGCCAAATGACTTACG
CTTGATGATTAAACTACGTAATAACGAGCAACAAGGCGCAGGCTTCTTACAAGGTGTGACCATTGATGGCCTTGATGACA
AAGGCAATATGACTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

57.56

99.735

0.574
  pilU Pseudomonas stutzeri DSM 10701

57.6

99.206

0.571
  pilU Vibrio cholerae strain A1552

56.131

97.09

0.545
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.944

95.238

0.399

Multiple sequence alignment