Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PSM_RS12640 Genome accession   NC_014803
Coordinates   2753798..2754838 (-) Length   346 a.a.
NCBI ID   WP_013465851.1    Uniprot ID   A0AA37S294
Organism   Pseudoalteromonas sp. SM9913     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2748798..2759838
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSM_RS12610 (PSM_A2575) - 2749140..2749613 (+) 474 WP_013465845.1 hypothetical protein -
  PSM_RS12615 (PSM_A2576) rsmE 2749684..2750418 (+) 735 WP_013465846.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PSM_RS12620 (PSM_A2577) gshB 2750485..2751435 (+) 951 WP_013465847.1 glutathione synthase -
  PSM_RS12625 (PSM_A2578) - 2751571..2752128 (+) 558 WP_013465848.1 YqgE/AlgH family protein -
  PSM_RS12630 (PSM_A2579) ruvX 2752146..2752595 (+) 450 WP_013465849.1 Holliday junction resolvase RuvX -
  PSM_RS12635 (PSM_A2580) pilU 2752652..2753788 (-) 1137 WP_013465850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PSM_RS12640 (PSM_A2581) pilT 2753798..2754838 (-) 1041 WP_013465851.1 type IV pilus twitching motility protein PilT Machinery gene
  PSM_RS12645 (PSM_A2582) - 2754841..2755554 (+) 714 WP_013465852.1 YggS family pyridoxal phosphate-dependent enzyme -
  PSM_RS12650 (PSM_A2583) proC 2755572..2756393 (+) 822 WP_013465853.1 pyrroline-5-carboxylate reductase -
  PSM_RS12655 (PSM_A2584) - 2756420..2756953 (+) 534 WP_008113334.1 YggT family protein -
  PSM_RS12660 (PSM_A2585) - 2757012..2757455 (+) 444 WP_013465854.1 DUF4426 domain-containing protein -
  PSM_RS12665 (PSM_A2586) - 2757458..2758057 (+) 600 WP_016900586.1 XTP/dITP diphosphatase -
  PSM_RS12670 (PSM_A2587) hemW 2758054..2759190 (+) 1137 WP_013465856.1 radical SAM family heme chaperone HemW -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38244.49 Da        Isoelectric Point: 6.2312

>NTDB_id=39158 PSM_RS12640 WP_013465851.1 2753798..2754838(-) (pilT) [Pseudoalteromonas sp. SM9913]
MDITELLAFSVQHKASDLHLSSGVSPMIRVDGDVRRINIPALGDKEVSSLVYDIMNDNQRKDYEQNLEVDFSFEVPNLAR
FRVNAFNSNRGPAAVFRTIPSDVLTLEDLGAPEIFKQISDNPRGLVLVTGPTGSGKSTTLAAMVDYINQTKHHHVLTIED
PIEFVHDNKLSLINQREVHRDTHSFSNALRSALREDPDVILVGELRDLETIRLAMTAAETGHLVFGTLHTTSAPKTIDRI
IDVFPGEEKDMIRSMLSESLRAVISQTLIKKVGGGRVAAHEIMLATPAIRNLIREDKIAQMYSSIQTGASQGMQTMDQCL
TSLVNRGIITHSAAYTKAQDKTQFSA

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=39158 PSM_RS12640 WP_013465851.1 2753798..2754838(-) (pilT) [Pseudoalteromonas sp. SM9913]
ATGGATATCACCGAATTATTGGCATTTAGTGTGCAACATAAAGCATCAGATTTGCACTTATCATCAGGGGTATCACCTAT
GATACGTGTTGATGGCGATGTGCGACGTATTAATATACCAGCACTTGGAGATAAAGAAGTCAGCAGTCTGGTTTATGACA
TTATGAACGATAACCAGCGTAAGGACTATGAACAAAATCTCGAAGTGGATTTTTCTTTTGAAGTCCCTAATTTAGCACGT
TTTCGTGTAAATGCCTTTAACTCTAATCGTGGCCCTGCTGCTGTGTTTCGTACTATCCCGAGTGACGTACTCACTCTTGA
AGATTTAGGCGCACCTGAAATATTTAAACAAATTTCAGATAACCCACGAGGCCTAGTGTTAGTCACGGGCCCTACAGGTT
CAGGTAAGTCGACCACGCTTGCGGCGATGGTCGATTACATTAACCAAACTAAGCATCATCATGTGCTGACTATTGAAGAT
CCTATCGAATTTGTTCACGATAATAAACTCAGCTTAATTAATCAGCGGGAAGTTCATCGTGATACACATAGCTTTTCTAA
CGCACTACGCAGCGCACTCCGTGAAGACCCTGATGTTATTTTAGTGGGTGAGTTACGAGATTTAGAAACTATTCGCCTTG
CTATGACCGCTGCGGAAACCGGTCACTTGGTGTTTGGTACTTTGCATACGACCTCTGCACCTAAAACCATTGACCGTATT
ATTGATGTATTCCCTGGCGAAGAAAAAGATATGATCCGCTCTATGCTCTCTGAGTCATTACGTGCAGTTATTTCGCAAAC
ACTGATAAAAAAAGTAGGGGGAGGGCGAGTGGCTGCACACGAAATTATGTTGGCAACGCCTGCTATTCGTAACTTAATCC
GTGAAGATAAAATTGCACAAATGTATTCATCAATTCAAACAGGTGCGTCGCAAGGCATGCAAACCATGGATCAATGTTTA
ACAAGCTTAGTTAACCGTGGCATTATCACTCACTCAGCGGCGTATACAAAAGCGCAAGATAAGACGCAGTTTAGCGCTTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

76.453

99.422

0.76
  pilT Pseudomonas aeruginosa PAK

75.872

99.422

0.754
  pilT Acinetobacter nosocomialis M2

75.872

99.422

0.754
  pilT Acinetobacter baumannii D1279779

75.872

99.422

0.754
  pilT Acinetobacter baumannii strain A118

75.872

99.422

0.754
  pilT Acinetobacter baylyi ADP1

75.291

99.422

0.749
  pilT Legionella pneumophila strain Lp02

71.802

99.422

0.714
  pilT Legionella pneumophila strain ERS1305867

71.802

99.422

0.714
  pilT Neisseria gonorrhoeae MS11

69.942

100

0.699
  pilT Neisseria meningitidis 8013

69.942

100

0.699
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

73.252

95.087

0.697
  pilT Vibrio cholerae strain A1552

73.252

95.087

0.697
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.312

100

0.523
  pilU Vibrio cholerae strain A1552

42.154

93.931

0.396
  pilU Acinetobacter baylyi ADP1

38.824

98.266

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.643

97.977

0.379

Multiple sequence alignment