Detailed information NTG0390736

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: F7R97_RS04500
• Genome accession: NZ_CP044517
• Coordinates: 953642..954760 (-)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii strain 31FS3-2
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 948642..959760

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F7R97_RS04470 (F7R97_04535)	-	949471..950319 (+)	849	WP_000336554.1	class II glutamine amidotransferase	-
F7R97_RS04475 (F7R97_04540)	-	950572..951027 (+)	456	WP_000782976.1	bacteriohemerythrin	-
F7R97_RS04480 (F7R97_04550)	-	951180..952247 (+)	1068	WP_002084046.1	hypothetical protein	-
F7R97_RS04485 (F7R97_04555)	-	952247..952570 (+)	324	WP_005139957.1	RnfH family protein	-
F7R97_RS04490 (F7R97_04560)	-	952625..953023 (-)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
F7R97_RS04495 (F7R97_04565)	fur	953135..953572 (+)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
F7R97_RS04500 (F7R97_04570)	pilU	953642..954760 (-)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
F7R97_RS04505 (F7R97_04575)	pilT	954788..955825 (-)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
F7R97_RS04510 (F7R97_04580)	-	955952..956644 (+)	693	WP_140952350.1	YggS family pyridoxal phosphate-dependent enzyme	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=390736 F7R97_RS04500 WP_000347034.1 953642..954760(-) (pilU) [Acinetobacter baumannii strain 31FS3-2]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=390736 F7R97_RS04500 WP_000347034.1 953642..954760(-) (pilU) [Acinetobacter baumannii strain 31FS3-2]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39