Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   F6A13_RS01215 Genome accession   NZ_CP044411
Coordinates   222103..223812 (+) Length   569 a.a.
NCBI ID   WP_126604582.1    Uniprot ID   A0A5J6VRB6
Organism   Acidithiobacillus sp. 'AMD consortium'     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217103..228812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F6A13_RS01190 (F6A13_01195) xth 217686..218459 (+) 774 WP_113526248.1 exodeoxyribonuclease III -
  F6A13_RS01195 (F6A13_01200) hemH 218456..219469 (+) 1014 WP_113526249.1 ferrochelatase -
  F6A13_RS01200 (F6A13_01205) - 219538..220026 (+) 489 WP_113526250.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  F6A13_RS01205 (F6A13_01210) - 220093..221256 (+) 1164 WP_232027826.1 anhydro-N-acetylmuramic acid kinase -
  F6A13_RS01210 (F6A13_01215) aroE 221237..222133 (+) 897 WP_113526252.1 shikimate dehydrogenase -
  F6A13_RS01215 (F6A13_01220) pilB 222103..223812 (+) 1710 WP_126604582.1 type IV-A pilus assembly ATPase PilB Machinery gene
  F6A13_RS01220 (F6A13_01225) - 223935..225539 (+) 1605 WP_225981898.1 two-component system sensor histidine kinase NtrB -
  F6A13_RS01225 (F6A13_01230) - 225532..226887 (+) 1356 WP_113526254.1 sigma-54-dependent transcriptional regulator -
  F6A13_RS01230 (F6A13_01235) - 226884..227684 (+) 801 WP_113526255.1 prepilin peptidase -
  F6A13_RS01235 (F6A13_01240) coaE 227685..228299 (+) 615 WP_126604581.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62717.42 Da        Isoelectric Point: 6.1150

>NTDB_id=389817 F6A13_RS01215 WP_126604582.1 222103..223812(+) (pilB) [Acidithiobacillus sp. 'AMD consortium']
MATNPEHISLTPVLRALVSNGLSDETRLQSLAADPARGKTPLLFYVVEKGAVPAAVLMAHLSARYNMPMLDLDAAAIDAL
LIRKLDKGLMTRYLVLPLSKHGDTLYLAMADPTDFKAVEDVKFNSGLQVMPVLVEADKLVKAVHAAVNSLQGGIDDVFVD
KPHGEEDPEEFDLAQENDGSVEDAPVVRFVRQLLLDAIQREVSDIHLEPYEKDFRVRYRLDGVLQDALHPPVALRDGVTS
RLKILCRLDISERRLPQDGRLRVRVPPARVIDFRVSFLPTSFGETIVLRLLDPASSRVPIEQLGFLPEQRKAFEDAIHRP
YGMILVTGPTGSGKTTTLYTALNILNTGDCNISTAEDPVEIPVYGINQVNINERIGLTFAAALRSFLRQDPDIIMVGEVR
DLETAETAIKAAQTGHLVLATLHTNDAPQSLTRLENMGIPTYNIAGSVHLVMAQRLVRRLCPHCKKPLRIPEQALIEAGL
APEDLQGWRPLGAVGCEQCNKTGYKGRMGLYQVMPVSDTMREIIMRGGTSIDLARAAAQEGVLTMRQNGLRRIREGVTSL
EEVLRVTNL

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=389817 F6A13_RS01215 WP_126604582.1 222103..223812(+) (pilB) [Acidithiobacillus sp. 'AMD consortium']
ATGGCTACAAACCCGGAACACATCTCGTTAACGCCAGTATTGCGGGCGCTCGTCAGTAACGGTTTAAGCGACGAGACGCG
CTTGCAAAGCCTGGCCGCCGATCCTGCCCGCGGTAAGACGCCGCTGCTGTTTTATGTGGTGGAGAAGGGCGCCGTCCCGG
CCGCGGTGCTCATGGCGCACCTTTCCGCGCGCTACAACATGCCCATGCTCGATCTCGACGCAGCGGCGATCGATGCGTTG
CTCATCCGCAAGCTCGACAAAGGGCTGATGACCCGCTATCTGGTGCTGCCCTTATCGAAGCATGGGGACACCCTCTACCT
CGCCATGGCCGATCCCACGGACTTCAAGGCGGTGGAGGATGTGAAATTCAATTCCGGTTTGCAGGTGATGCCGGTGCTGG
TTGAGGCCGACAAACTGGTCAAGGCGGTACATGCTGCGGTCAACAGCTTGCAGGGCGGGATTGACGACGTATTCGTGGAT
AAGCCGCACGGTGAGGAAGATCCGGAAGAATTCGACCTCGCCCAGGAGAACGACGGTAGTGTCGAGGATGCGCCGGTGGT
GCGCTTTGTCCGGCAACTGCTGCTGGATGCCATTCAGCGCGAGGTGTCCGATATTCACCTGGAACCCTATGAAAAGGATT
TTCGGGTACGTTACCGCCTCGATGGCGTATTGCAGGATGCGCTGCATCCGCCCGTGGCGTTGCGGGACGGGGTCACCTCG
CGCCTGAAGATTCTTTGCCGTCTGGACATTTCCGAGCGACGTCTGCCGCAGGACGGCCGACTGCGGGTCCGCGTGCCCCC
GGCCCGGGTCATCGATTTTCGCGTCTCCTTTCTGCCCACCAGTTTTGGCGAAACGATCGTGCTGCGCCTGCTGGATCCCG
CCAGTTCCAGGGTGCCCATCGAGCAACTGGGCTTTTTACCAGAGCAGCGCAAAGCCTTTGAGGACGCCATCCATCGGCCC
TACGGCATGATTCTGGTCACCGGGCCCACGGGTTCCGGCAAGACCACGACCCTCTATACCGCCCTGAACATCCTCAACAC
CGGTGACTGCAACATCAGTACCGCAGAGGACCCCGTGGAAATCCCGGTGTACGGGATCAATCAGGTGAACATCAACGAGC
GCATCGGTCTCACCTTTGCCGCAGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGATATCATCATGGTCGGCGAGGTGCGC
GATCTGGAAACCGCCGAAACCGCCATCAAGGCAGCCCAGACGGGCCATCTGGTATTGGCCACCCTGCACACCAATGATGC
TCCACAGAGTCTCACTCGCCTCGAGAACATGGGTATTCCCACCTATAACATCGCCGGCAGCGTACATCTGGTGATGGCGC
AGCGACTCGTCCGCAGGCTTTGCCCGCACTGTAAAAAACCCCTGCGCATTCCCGAACAGGCCCTGATCGAGGCGGGACTT
GCGCCCGAGGATCTGCAGGGCTGGCGCCCTCTGGGCGCTGTGGGCTGCGAACAGTGCAATAAAACCGGATATAAGGGCCG
GATGGGGCTTTATCAGGTGATGCCGGTGAGCGACACCATGCGCGAAATCATCATGCGTGGCGGCACGTCCATCGACTTGG
CGCGCGCCGCGGCACAGGAGGGGGTGCTGACCATGCGGCAGAATGGGCTGAGGCGGATCCGGGAGGGCGTGACCAGTCTG
GAGGAGGTGCTGCGGGTGACCAATCTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-143)

Type II/IV secretion system protein (T2SSE)

(193-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5J6VRB6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

48.348

100

0.489
  pilB Acinetobacter baumannii D1279779

48.239

99.824

0.482
  pilB Acinetobacter baylyi ADP1

47.359

99.824

0.473
  pilF Neisseria gonorrhoeae MS11

45.633

98.594

0.45
  pilB Vibrio cholerae strain A1552

44.207

98.594

0.436
  pilB Vibrio parahaemolyticus RIMD 2210633

42.73

99.121

0.424
  pilB Vibrio campbellii strain DS40M4

44.677

92.443

0.413
  pilF Thermus thermophilus HB27

39.502

98.77

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.695

92.091

0.366

Multiple sequence alignment