Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   HHJ28_RS03755 Genome accession   NZ_CP051727
Coordinates   788302..789795 (+) Length   497 a.a.
NCBI ID   WP_000249335.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-111     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783302..794795
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ28_RS03735 (HHJ28_03735) pilD 783950..784759 (+) 810 WP_000895875.1 prepilin peptidase PppA Machinery gene
  HHJ28_RS03740 (HHJ28_03740) gspS2 784825..785235 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  HHJ28_RS03745 (HHJ28_03745) gspC 785253..786212 (+) 960 WP_001764943.1 type II secretion system protein GspC -
  HHJ28_RS03750 (HHJ28_03750) gspD 786242..788302 (+) 2061 WP_000498844.1 type II secretion system secretin GspD -
  HHJ28_RS03755 (HHJ28_03755) pilB/pilB1 788302..789795 (+) 1494 WP_000249335.1 type II secretion system ATPase GspE Machinery gene
  HHJ28_RS03760 (HHJ28_03760) gspF 789795..791018 (+) 1224 WP_001173412.1 type II secretion system inner membrane protein GspF -
  HHJ28_RS03765 (HHJ28_03765) gspG 791035..791490 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  HHJ28_RS03770 (HHJ28_03770) gspH 791494..792057 (+) 564 WP_001764944.1 type II secretion system minor pseudopilin GspH -
  HHJ28_RS03775 (HHJ28_03775) gspI 792054..792425 (+) 372 WP_000820086.1 type II secretion system minor pseudopilin GspI -
  HHJ28_RS03780 (HHJ28_03780) gspJ 792422..793027 (+) 606 WP_001250460.1 type II secretion system minor pseudopilin GspJ -
  HHJ28_RS03785 (HHJ28_03785) gspK 793024..794001 (+) 978 WP_135149979.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55129.20 Da        Isoelectric Point: 5.9800

>NTDB_id=387482 HHJ28_RS03755 WP_000249335.1 788302..789795(+) (pilB/pilB1) [Escherichia coli strain SCU-111]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=387482 HHJ28_RS03755 WP_000249335.1 788302..789795(+) (pilB/pilB1) [Escherichia coli strain SCU-111]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAACTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTGAGTCACGAACTGACC
GCGCAGTTGCGTCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACCCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

47.118

80.282

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilB Acinetobacter baylyi ADP1

46.667

78.471

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362