Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHJ31_RS11495 Genome accession   NZ_CP051719
Coordinates   2374572..2376053 (+) Length   493 a.a.
NCBI ID   WP_001219891.1    Uniprot ID   B7MCU1
Organism   Escherichia coli strain SCU-116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2369572..2381053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ31_RS11475 (HHJ31_11475) gspB 2369741..2370160 (-) 420 WP_000461443.1 putative general secretion pathway protein GspB -
  HHJ31_RS11480 (HHJ31_11480) gspA 2370162..2371631 (-) 1470 WP_000107586.1 type II secretion system protein GspA -
  HHJ31_RS11485 (HHJ31_11485) gspC 2371811..2372626 (+) 816 WP_001142674.1 type II secretion system protein GspC -
  HHJ31_RS11490 (HHJ31_11490) gspD 2372610..2374562 (+) 1953 WP_001514015.1 type II secretion system secretin GspD -
  HHJ31_RS11495 (HHJ31_11495) pilB 2374572..2376053 (+) 1482 WP_001219891.1 type II secretion system ATPase GspE Machinery gene
  HHJ31_RS11500 (HHJ31_11500) gspF 2376050..2377246 (+) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  HHJ31_RS11505 (HHJ31_11505) gspG 2377256..2377693 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  HHJ31_RS11510 (HHJ31_11510) gspH 2377701..2378210 (+) 510 WP_001076038.1 type II secretion system minor pseudopilin GspH -
  HHJ31_RS11515 (HHJ31_11515) gspI 2378207..2378602 (+) 396 WP_001041747.1 type II secretion system minor pseudopilin GspI -
  HHJ31_RS11520 (HHJ31_11520) gspJ 2378595..2379182 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  HHJ31_RS11525 (HHJ31_11525) gspK 2379175..2380158 (+) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -
  HHJ31_RS11530 (HHJ31_11530) gspL 2380173..2380646 (+) 474 Protein_2281 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54666.59 Da        Isoelectric Point: 6.3224

>NTDB_id=387322 HHJ31_RS11495 WP_001219891.1 2374572..2376053(+) (pilB) [Escherichia coli strain SCU-116]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
HHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=387322 HHJ31_RS11495 WP_001219891.1 2374572..2376053(+) (pilB) [Escherichia coli strain SCU-116]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGGCTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAAGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGACGTATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTGCTCCGTCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATCCAGCTT
CCACACGGTATTATCCTGGTGACCGGACCGACAGGCTCCGGTAAAAGTACCACGCTCTACGCCATTCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCTGTATCGCCGCAACAAGCGCAGATGTTTAAA
CATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTACCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7MCU1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.697

89.047

0.416
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.588

86.207

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361

Multiple sequence alignment