Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HHJ32_RS01930 Genome accession   NZ_CP051716
Coordinates   422523..424004 (-) Length   493 a.a.
NCBI ID   WP_169059434.1    Uniprot ID   -
Organism   Escherichia coli strain SCU-118     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 417523..429004
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHJ32_RS01900 (HHJ32_01900) gspK 418436..419419 (-) 984 WP_001058522.1 type II secretion system minor pseudopilin GspK -
  HHJ32_RS01905 (HHJ32_01905) gspJ 419412..419999 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  HHJ32_RS01910 (HHJ32_01910) gspI 419992..420369 (-) 378 WP_001041743.1 type II secretion system minor pseudopilin GspI -
  HHJ32_RS01915 (HHJ32_01915) gspH 420366..420875 (-) 510 WP_001076046.1 type II secretion system minor pseudopilin GspH -
  HHJ32_RS01920 (HHJ32_01920) gspG 420883..421320 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -
  HHJ32_RS01925 (HHJ32_01925) gspF 421330..422526 (-) 1197 WP_001109568.1 type II secretion system inner membrane protein GspF -
  HHJ32_RS01930 (HHJ32_01930) pilB 422523..424004 (-) 1482 WP_169059434.1 type II secretion system ATPase GspE Machinery gene
  HHJ32_RS01935 (HHJ32_01935) gspD 424014..425966 (-) 1953 WP_001326512.1 type II secretion system secretin GspD -
  HHJ32_RS01940 (HHJ32_01940) gspC 425950..426765 (-) 816 WP_001142708.1 type II secretion system protein GspC -
  HHJ32_RS01945 (HHJ32_01945) gspA 426945..428414 (+) 1470 WP_000107576.1 type II secretion system protein GspA -
  HHJ32_RS01950 (HHJ32_01950) gspB 428416..428835 (+) 420 WP_000461450.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54596.54 Da        Isoelectric Point: 6.2412

>NTDB_id=387220 HHJ32_RS01930 WP_169059434.1 422523..424004(-) (pilB) [Escherichia coli strain SCU-118]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETGSDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHKALIKNGLQKVISGDTSWDE
VMRVASATLESEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=387220 HHJ32_RS01930 WP_169059434.1 422523..424004(-) (pilB) [Escherichia coli strain SCU-118]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAGCGGTTACTCGATATTCAACGTCAGGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCTCGGCTGGAAGCGGTATTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCGATTTTGAGTGAGGCCATCAAAGAAACCGGCTCTGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCAAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATTAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCGTCCATCTATGGTGAACGCGCCGTACTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAGCAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTGACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATCCTTTCGGCGCTGAA
TACTCCCGGCCGCAATATTCTGACGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATA
CCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGATCCGGATGTCGTCATGGTGGGGGAAATT
CGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCCCTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGCCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCACCCCAACAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACAACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTATCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTACGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTAGTCCGGCAACAACACAAGGCCTTAATCAAAAATGGCCTGCAAAAAGTGATAAGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAGCGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.453

88.641

0.412
  pilB Vibrio cholerae strain A1552

48.201

84.584

0.408
  pilB Acinetobacter baumannii D1279779

45.351

89.452

0.406
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.118

86.207

0.398
  pilB/pilB1 Synechocystis sp. PCC 6803

41.176

96.552

0.398
  pilB Acinetobacter baylyi ADP1

48.082

79.31

0.381
  pilF Thermus thermophilus HB27

45.063

80.122

0.361