Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CVCAS_RS10705 Genome accession   NC_017486
Coordinates   2143727..2144173 (-) Length   148 a.a.
NCBI ID   WP_029344525.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis CV56     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2138727..2149173
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CVCAS_RS10670 (CVCAS_2095) - 2138835..2139212 (-) 378 WP_003129983.1 pyridoxamine 5'-phosphate oxidase family protein -
  CVCAS_RS10675 (CVCAS_2096) - 2139416..2140282 (+) 867 WP_003129984.1 RluA family pseudouridine synthase -
  CVCAS_RS10680 (CVCAS_2097) - 2140321..2141130 (-) 810 WP_014570791.1 metal ABC transporter permease -
  CVCAS_RS10685 (CVCAS_2098) - 2141123..2141860 (-) 738 WP_003129989.1 metal ABC transporter ATP-binding protein -
  CVCAS_RS10690 (CVCAS_2099) - 2142037..2142879 (-) 843 WP_003129990.1 metal ABC transporter substrate-binding protein -
  CVCAS_RS10695 (CVCAS_2100) - 2142876..2143313 (-) 438 WP_003129992.1 zinc-dependent MarR family transcriptional regulator -
  CVCAS_RS10700 (CVCAS_2101) comGG 2143395..2143688 (-) 294 WP_043991170.1 competence type IV pilus minor pilin ComGG Machinery gene
  CVCAS_RS10705 (CVCAS_2102) comGF 2143727..2144173 (-) 447 WP_029344525.1 competence type IV pilus minor pilin ComGF Machinery gene
  CVCAS_RS10710 (CVCAS_2103) comGE 2144136..2144432 (-) 297 WP_010906316.1 competence type IV pilus minor pilin ComGE Machinery gene
  CVCAS_RS10715 (CVCAS_2104) comGD 2144404..2144835 (-) 432 WP_014570794.1 competence type IV pilus minor pilin ComGD Machinery gene
  CVCAS_RS10720 (CVCAS_2105) comGC 2144795..2145064 (-) 270 WP_003129998.1 competence type IV pilus major pilin ComGC Machinery gene
  CVCAS_RS10725 (CVCAS_2106) - 2145211..2145735 (-) 525 WP_014570795.1 GNAT family N-acetyltransferase -
  CVCAS_RS10730 (CVCAS_2107) - 2145814..2146593 (-) 780 WP_014570796.1 peptidoglycan amidohydrolase family protein -
  CVCAS_RS10735 (CVCAS_2108) - 2146593..2146892 (-) 300 WP_014570797.1 phage holin -
  CVCAS_RS10740 (CVCAS_2109) - 2146905..2147255 (-) 351 WP_014570798.1 hypothetical protein -
  CVCAS_RS10745 (CVCAS_2110) - 2147268..2147504 (-) 237 WP_014570799.1 hypothetical protein -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 17111.91 Da        Isoelectric Point: 8.6780

>NTDB_id=38498 CVCAS_RS10705 WP_029344525.1 2143727..2144173(-) (comGF) [Lactococcus lactis subsp. lactis CV56]
MERKLCDLNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide


Download         Length: 447 bp        

>NTDB_id=38498 CVCAS_RS10705 WP_029344525.1 2143727..2144173(-) (comGF) [Lactococcus lactis subsp. lactis CV56]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGTCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

Putative Competence protein ComGF (ComGF)

(48-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Lactococcus lactis subsp. cremoris KW2

76.259

93.919

0.716
  comGF Streptococcus mutans UA140

48.227

95.27

0.459
  comGF Streptococcus mutans UA159

47.518

95.27

0.453
  comGF/cglF Streptococcus mitis SK321

43.066

92.568

0.399
  comGF/cglF Streptococcus pneumoniae Rx1

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae D39

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae R6

40.426

95.27

0.385
  comGF/cglF Streptococcus pneumoniae TIGR4

40.426

95.27

0.385
  comGF/cglF Streptococcus mitis NCTC 12261

41.606

92.568

0.385

Multiple sequence alignment