Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XOCgx_RS05785 Genome accession   NZ_CP043403
Coordinates   1202159..1203892 (+) Length   577 a.a.
NCBI ID   WP_024711610.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzicola strain GX01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1197159..1208892
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XOCgx_RS05765 (XOCgx_1256) pilA2 1197838..1198263 (+) 426 WP_047340102.1 pilin Machinery gene
  XOCgx_RS05770 (XOCgx_1257) - 1198399..1199988 (+) 1590 WP_014504322.1 phosphoethanolamine transferase -
  XOCgx_RS05775 (XOCgx_1258) - 1199999..1200628 (+) 630 WP_024711608.1 hypothetical protein -
  XOCgx_RS05780 (XOCgx_1259) - 1200598..1202124 (+) 1527 WP_044749930.1 membrane protein -
  XOCgx_RS05785 (XOCgx_1260) pilB 1202159..1203892 (+) 1734 WP_024711610.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XOCgx_RS05790 (XOCgx_1261) - 1203901..1204410 (-) 510 WP_193566026.1 Fic family protein -
  XOCgx_RS05795 (XOCgx_1262) - 1204472..1204678 (-) 207 WP_076342549.1 YhfG family protein -
  XOCgx_RS05800 (XOCgx_1263) pilR 1204751..1206202 (-) 1452 WP_178385942.1 sigma-54 dependent transcriptional regulator Regulator
  XOCgx_RS05805 (XOCgx_1264) - 1206473..1208086 (-) 1614 WP_014504316.1 HAMP domain-containing sensor histidine kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62966.04 Da        Isoelectric Point: 5.6097

>NTDB_id=383466 XOCgx_RS05785 WP_024711610.1 1202159..1203892(+) (pilB) [Xanthomonas oryzae pv. oryzicola strain GX01]
MNSVSTTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKRLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDEEMGDLDVSTGDEDIGTGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=383466 XOCgx_RS05785 WP_024711610.1 1202159..1203892(+) (pilB) [Xanthomonas oryzae pv. oryzicola strain GX01]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
GGCCGCTGCGCGGACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAGGCTGG
TGTCGGCGTCGCAGCTGGCGGCGGCCAATGCAGTGGAATTTGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTTCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATTCGTCGCACATTGGAGCAGTGGCAGGCAAGCAATGCCTCATTTGGATCATCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTCTCCACCGGCGACGAAGACATAGGCACCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTTGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGCCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGTGAAA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGCT
GTACACGGCGCTGGGCATCCTCAACGATGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GATATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGCTGG
AAGGCGGCAATGCGATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.942

98.614

0.562
  pilB Acinetobacter baylyi ADP1

57.042

98.44

0.562
  pilB Legionella pneumophila strain ERS1305867

52.113

98.44

0.513
  pilB Vibrio cholerae strain A1552

50.087

100

0.501
  pilF Neisseria gonorrhoeae MS11

49.648

98.44

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

46.986

97.747

0.459
  pilB Vibrio campbellii strain DS40M4

46.087

99.653

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.508

0.36

Multiple sequence alignment