Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   FYN22_RS08160 Genome accession   NZ_CP043307
Coordinates   1709064..1710257 (-) Length   397 a.a.
NCBI ID   WP_148826096.1    Uniprot ID   -
Organism   Acinetobacter johnsonii strain Acsw19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1704064..1715257
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYN22_RS08145 (FYN22_08145) - 1706098..1707126 (+) 1029 WP_148826094.1 AraC family transcriptional regulator -
  FYN22_RS08150 (FYN22_08150) gcvH 1707198..1707572 (+) 375 WP_004693240.1 glycine cleavage system protein GcvH -
  FYN22_RS08155 (FYN22_08155) - 1707838..1709016 (+) 1179 WP_148826095.1 MFS transporter -
  FYN22_RS08160 (FYN22_08160) pilU 1709064..1710257 (-) 1194 WP_148826096.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  FYN22_RS08165 (FYN22_08165) - 1710496..1711557 (-) 1062 WP_148826097.1 3-deoxy-7-phosphoheptulonate synthase -
  FYN22_RS08170 (FYN22_08170) - 1712048..1714147 (-) 2100 WP_148826098.1 EAL domain-containing protein -

Sequence

Protein


Download         Length: 397 a.a.        Molecular weight: 44652.01 Da        Isoelectric Point: 6.5064

>NTDB_id=382766 FYN22_RS08160 WP_148826096.1 1709064..1710257(-) (pilU) [Acinetobacter johnsonii strain Acsw19]
MYSAELLEEARRMMFHMLSKVVEYGGSDLFISADFPPSIKHQGLMKPLGQQNLPSDQTKLFAYSLMNEKQRLEFETELEC
NFAISVPNVSRFRVNVFQQQLHVGMVIRTITAEIPNFTKLQIPTSLKDVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPDERRNQLLMDLSSNMKAIISQRLVRTEDGRGRRAAVEIMLNTPLMSDLILKGNFHELKEVMSKS
RELGMQTFDQALFDLYNQGAIAYEEALRNADSVNELRLQIKLKSSRANSQLNSSSALSFDQAVAEKAKEAEEEKADA

Nucleotide


Download         Length: 1194 bp        

>NTDB_id=382766 FYN22_RS08160 WP_148826096.1 1709064..1710257(-) (pilU) [Acinetobacter johnsonii strain Acsw19]
ATGTATTCTGCAGAACTTTTAGAAGAAGCCCGTCGGATGATGTTTCACATGCTCAGCAAAGTGGTTGAGTATGGTGGTTC
AGACTTATTTATATCTGCAGACTTTCCGCCAAGTATTAAGCATCAGGGCCTCATGAAGCCACTGGGTCAACAAAACTTAC
CATCTGATCAGACCAAACTCTTTGCTTATAGTTTAATGAACGAAAAGCAACGATTAGAGTTCGAAACTGAGCTGGAGTGT
AATTTTGCAATTAGCGTTCCCAATGTCTCACGTTTTCGCGTCAATGTTTTTCAGCAGCAACTACATGTAGGTATGGTGAT
TCGTACGATTACCGCGGAAATTCCTAACTTCACCAAGTTACAAATTCCAACGTCTTTAAAAGACGTGATTATGGAAAAAC
GTGGTTTAGTATTGGTAGTTGGCGGTACAGGGTCGGGTAAATCGACATCTTTGGCGGCCATGATTGATCATCGAAATGAA
AACTCGGCAGGGCACATTATTACGGTTGAAGACCCAGTGGAATATGTTCACAAGCATAAGAAATCGATGATTACTCATCG
AGAAGTAGGTGTGGACTGTCACTCTTGGCACAATGCTTTGAAAAATACTTTGCGCCAAGCACCTGATGTCATTTTAATTG
GTGAGATTCGTGACACGGAGACGATGGAGCATGCCATAGCTTTTGCAGAAACAGGCCACTTATGCCTAGGCACATTACAT
GCTAATAATGCAAATCAGGCTTTAGATCGAATTATTAACTTTTTCCCAGACGAACGTCGCAATCAACTTTTGATGGACTT
ATCTTCCAATATGAAGGCGATTATTTCACAGCGTCTGGTGCGAACTGAAGATGGGCGTGGCCGCCGAGCAGCAGTAGAAA
TCATGCTTAATACACCGTTGATGTCAGACTTGATACTTAAAGGGAATTTCCATGAACTCAAAGAAGTGATGTCGAAGTCA
CGAGAACTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATTTATACAATCAAGGTGCAATTGCTTACGAAGAAGCGCT
ACGAAATGCAGACTCGGTCAATGAATTACGTCTACAGATTAAATTAAAGAGTAGTCGTGCTAACTCACAGTTAAACTCAA
GTAGTGCTTTGTCTTTTGATCAAGCGGTTGCCGAAAAGGCCAAAGAAGCTGAAGAAGAGAAGGCGGACGCCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.053

90.428

0.534
  pilU Acinetobacter baylyi ADP1

54.545

91.436

0.499
  pilU Vibrio cholerae strain A1552

50.289

87.154

0.438
  pilT Legionella pneumophila strain Lp02

42.183

85.39

0.36
  pilT Legionella pneumophila strain ERS1305867

42.183

85.39

0.36

Multiple sequence alignment