Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FWK45_RS01365 Genome accession   NZ_CP043001
Coordinates   269286..269744 (+) Length   152 a.a.
NCBI ID   WP_075293673.1    Uniprot ID   A0A9Q6Z0V5
Organism   Histophilus somni strain UOC-KLM-ATR-04     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264286..274744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK45_RS01335 (FWK45_01335) ispB 264307..265278 (-) 972 WP_075321735.1 octaprenyl diphosphate synthase -
  FWK45_RS01340 (FWK45_01340) rplU 265519..265830 (+) 312 WP_011608399.1 50S ribosomal protein L21 -
  FWK45_RS01345 (FWK45_01345) rpmA 265851..266108 (+) 258 WP_011608400.1 50S ribosomal protein L27 -
  FWK45_RS01350 (FWK45_01350) - 266244..267164 (+) 921 WP_012340377.1 DMT family transporter -
  FWK45_RS01355 (FWK45_01355) cgtA 267174..268352 (+) 1179 WP_132995389.1 Obg family GTPase CgtA -
  FWK45_RS01360 (FWK45_01360) ampD 268594..269163 (-) 570 WP_012340392.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FWK45_RS01365 (FWK45_01365) pilA 269286..269744 (+) 459 WP_075293673.1 prepilin peptidase-dependent pilin Machinery gene
  FWK45_RS01370 (FWK45_01370) artP 269890..270627 (+) 738 WP_012340409.1 arginine ABC transporter ATP-binding protein ArtP -
  FWK45_RS01375 (FWK45_01375) - 270645..271370 (+) 726 WP_012340415.1 transporter substrate-binding domain-containing protein -
  FWK45_RS01380 (FWK45_01380) artQ 271375..272046 (+) 672 WP_132995387.1 arginine ABC transporter permease ArtQ -
  FWK45_RS01385 (FWK45_01385) artM 272057..272740 (+) 684 WP_011608408.1 arginine ABC transporter permease ArtM -
  FWK45_RS01390 (FWK45_01390) - 272846..273097 (-) 252 WP_233120535.1 DUF5339 domain-containing protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15875.12 Da        Isoelectric Point: 8.4638

>NTDB_id=380080 FWK45_RS01365 WP_075293673.1 269286..269744(+) (pilA) [Histophilus somni strain UOC-KLM-ATR-04]
MKITYIFSSAKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITELLQASAPYRAEVELCVYNNDAPTNCNAGTNGI
QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide


Download         Length: 459 bp        

>NTDB_id=380080 FWK45_RS01365 WP_075293673.1 269286..269744(+) (pilA) [Histophilus somni strain UOC-KLM-ATR-04]
ATGAAAATAACCTATATTTTTTCATCAGCTAAAAAAGGATTTACTCTCATAGAACTGATGATCGTTATTGCTATTGTGGC
AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGCTGTTACAAGCTTCTG
CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT
CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG
AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA
ATAAGGCCGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

65.306

96.711

0.632
  pilA Haemophilus influenzae Rd KW20

63.265

96.711

0.612
  pilA Glaesserella parasuis strain SC1401

50.323

100

0.513
  pilA Ralstonia pseudosolanacearum GMI1000

35.119

100

0.388
  pilA Vibrio campbellii strain DS40M4

38.356

96.053

0.368

Multiple sequence alignment