Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FWK62_RS01385 Genome accession   NZ_CP042993
Coordinates   269290..269748 (+) Length   152 a.a.
NCBI ID   WP_075293673.1    Uniprot ID   A0A9Q6Z0V5
Organism   Histophilus somni strain UOC-KLM-ATR-014     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 264290..274748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FWK62_RS01355 (FWK62_01355) ispB 264310..265281 (-) 972 WP_041603986.1 octaprenyl diphosphate synthase -
  FWK62_RS01360 (FWK62_01360) rplU 265522..265833 (+) 312 WP_011608399.1 50S ribosomal protein L21 -
  FWK62_RS01365 (FWK62_01365) rpmA 265854..266111 (+) 258 WP_011608400.1 50S ribosomal protein L27 -
  FWK62_RS01370 (FWK62_01370) - 266247..267167 (+) 921 WP_075321736.1 DMT family transporter -
  FWK62_RS01375 (FWK62_01375) cgtA 267177..268355 (+) 1179 WP_075321737.1 Obg family GTPase CgtA -
  FWK62_RS01380 (FWK62_01380) ampD 268598..269167 (-) 570 WP_075321738.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FWK62_RS01385 (FWK62_01385) pilA 269290..269748 (+) 459 WP_075293673.1 prepilin peptidase-dependent pilin Machinery gene
  FWK62_RS01390 (FWK62_01390) artP 269894..270631 (+) 738 WP_012340409.1 arginine ABC transporter ATP-binding protein ArtP -
  FWK62_RS01395 (FWK62_01395) - 270649..271374 (+) 726 WP_136690229.1 transporter substrate-binding domain-containing protein -
  FWK62_RS01400 (FWK62_01400) artQ 271379..272050 (+) 672 WP_132995387.1 arginine ABC transporter permease ArtQ -
  FWK62_RS01405 (FWK62_01405) artM 272061..272744 (+) 684 WP_011608408.1 arginine ABC transporter permease ArtM -
  FWK62_RS01410 (FWK62_01410) - 272850..273101 (-) 252 WP_233120535.1 DUF5339 domain-containing protein -

Sequence

Protein


Download         Length: 152 a.a.        Molecular weight: 15875.12 Da        Isoelectric Point: 8.4638

>NTDB_id=380003 FWK62_RS01385 WP_075293673.1 269290..269748(+) (pilA) [Histophilus somni strain UOC-KLM-ATR-014]
MKITYIFSSAKKGFTLIELMIVIAIVAILATIAIPSYQNYTKKAAITELLQASAPYRAEVELCVYNNDAPTNCNAGTNGI
QAAINTPKGKVKSVSVQGGAITVTGNGNLKDITYTLTPTGDASTGVTWSANCNNKADLFPAGFCSSTSTNSP

Nucleotide


Download         Length: 459 bp        

>NTDB_id=380003 FWK62_RS01385 WP_075293673.1 269290..269748(+) (pilA) [Histophilus somni strain UOC-KLM-ATR-014]
ATGAAAATAACCTATATTTTTTCATCAGCTAAAAAAGGATTTACTCTCATAGAACTGATGATCGTTATTGCTATTGTGGC
AATTCTTGCAACCATTGCAATTCCGTCATACCAAAATTATACAAAAAAAGCTGCAATTACAGAGCTGTTACAAGCTTCTG
CTCCTTATCGTGCAGAAGTTGAGCTATGCGTGTATAACAATGATGCACCGACAAATTGTAATGCTGGAACAAATGGAATT
CAAGCGGCAATCAATACACCAAAAGGTAAAGTAAAATCAGTTAGCGTGCAAGGTGGAGCCATTACTGTTACAGGGAATGG
AAATCTTAAAGATATTACTTATACCTTAACACCAACAGGCGATGCTTCAACCGGCGTTACTTGGAGTGCAAATTGCAATA
ATAAGGCTGATCTATTTCCTGCCGGATTTTGCTCTTCTACGTCTACAAACTCTCCTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

65.306

96.711

0.632
  pilA Haemophilus influenzae Rd KW20

63.265

96.711

0.612
  pilA Glaesserella parasuis strain SC1401

50.323

100

0.513
  pilA Ralstonia pseudosolanacearum GMI1000

35.119

100

0.388
  pilA Vibrio campbellii strain DS40M4

38.356

96.053

0.368

Multiple sequence alignment