Detailed information NTG0378442

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: FR761_RS17825
• Genome accession: NZ_CP042556
• Coordinates: 3654183..3655241 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain E47
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3649183..3660241

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FR761_RS17800 (FR761_17800)	aroK	3649458..3650027 (-)	570	WP_000643712.1	shikimate kinase AroK	-
FR761_RS17805 (FR761_17805)	pilQ	3650039..3652204 (-)	2166	WP_004742153.1	type IV pilus secretin PilQ family protein	Machinery gene
FR761_RS17810 (FR761_17810)	pilP	3652267..3652794 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
FR761_RS17815 (FR761_17815)	pilO	3652805..3653545 (-)	741	WP_000081156.1	type 4a pilus biogenesis protein PilO	Machinery gene
FR761_RS17820 (FR761_17820)	pilN	3653542..3654183 (-)	642	WP_000201224.1	PilN domain-containing protein	Machinery gene
FR761_RS17825 (FR761_17825)	pilM	3654183..3655241 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
FR761_RS17830 (FR761_17830)	ponA	3655404..3657959 (+)	2556	WP_057069721.1	penicillin-binding protein PBP1a	-
FR761_RS17835 (FR761_17835)	-	3657977..3658801 (-)	825	WP_002125608.1	putative RNA methyltransferase	-
FR761_RS17840 (FR761_17840)	-	3658804..3659136 (-)	333	WP_000169598.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=378442 FR761_RS17825 WP_002014066.1 3654183..3655241(-) (pilM) [Acinetobacter baumannii strain E47]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=378442 FR761_RS17825 WP_002014066.1 3654183..3655241(-) (pilM) [Acinetobacter baumannii strain E47]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCAGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423