Detailed information NTG0375127

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: G8B35_RS00345
• Genome accession: NZ_CP049697
• Coordinates: 55715..56713 (+)
• Length: 332 a.a.
• NCBI ID: WP_002986679.1
• Uniprot ID: A2RC05
• Organism: Streptococcus pyogenes strain ABC3
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 50715..61713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
G8B35_RS00325 (G8B35_00325)	-	51761..52645 (+)	885	WP_011528141.1	HNH endonuclease	-
G8B35_RS00330 (G8B35_00330)	purB	53154..54446 (+)	1293	WP_011528142.1	adenylosuccinate lyase	-
G8B35_RS00335 (G8B35_00335)	comR	54578..55489 (+)	912	WP_011528143.1	transcriptional regulator Rgg4/ComR	Regulator
G8B35_RS09680	comS	55573..55668 (+)	96	WP_198462994.1	quorum-sensing system DWW-type pheromone	Regulator
G8B35_RS00345 (G8B35_00340)	ruvB	55715..56713 (+)	999	WP_002986679.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
G8B35_RS00350 (G8B35_00345)	-	56851..57288 (+)	438	WP_002987727.1	low molecular weight protein-tyrosine-phosphatase	-
G8B35_RS00355 (G8B35_00350)	-	57312..57713 (+)	402	WP_002986671.1	MORN repeat-containing protein	-
G8B35_RS00360 (G8B35_00355)	-	57710..59485 (+)	1776	WP_011528145.1	acyltransferase family protein	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37565.11 Da
• Isoelectric Point: 5.3005

>NTDB_id=375127 G8B35_RS00345 WP_002986679.1 55715..56713(+) (ruvB) [Streptococcus pyogenes strain ABC3]
MARILDNNVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEAAKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNL
KQTSGPAVEKAGDLVAILNELEPGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPRIANRLLKRVRDYAQIIGDGIIT
AQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGGPVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQK
AYRHLGYPYQNT

Nucleotide

• Length: 999 bp

>NTDB_id=375127 G8B35_RS00345 WP_002986679.1 55715..56713(+) (ruvB) [Streptococcus pyogenes strain ABC3]
ATGGCTAGAATTTTAGACAATAATGTAATGGGAAATGAGGAGTTTTCGGATCGGACTCTTCGCCCTCAATATCTACACGA
ATATATTGGACAAGATAAAGTTAAGGAACAATTCGCAATTTTTATAGAAGCAGCTAAAAGACGTGACGAATCTTTAGACC
ATGTTTTATTATTTGGACCTCCAGGACTGGGAAAAACAACAATGGCATTTGTGATTGCCAATGAATTGGGAGTTAATTTA
AAGCAAACTTCAGGTCCTGCCGTTGAGAAAGCTGGAGATTTGGTAGCTATTTTAAATGAATTAGAACCAGGGGATATTTT
GTTTATTGATGAAATTCACCGCATGCCAATGTCTGTCGAGGAAGTACTATATAGTGCTATGGAAGACTTCTATATTGATA
TTATGATTGGCGCTGGAGATACTAGTCGTAGTATTCATTTGGATCTGCCTCCATTTACATTGATTGGTGCTACTACACGT
GCAGGCATGTTATCTAATCCCCTCAGAGCTCGTTTTGGTATCACTGGTCACATGGAATACTATCAAGAAAAGGATTTGAC
AGAGATTGTGGAGCGTACAGCTACTATTTTTGAAATAAAAATAGACCATGAAGCTGCACGGAAATTAGCTTGTCGCAGTA
GAGGAACGCCCCGCATTGCTAATCGATTACTAAAACGAGTGCGTGACTATGCTCAAATTATTGGTGATGGCATCATAACG
GCTCAAATAACTGATAGAGCTTTGACTATGTTAGATGTTGACCGCGAAGGTCTCGATTACATTGATCAGAAAATTTTACG
AACGATGATTGAGATGTATCAGGGAGGACCAGTTGGTTTGGGGACATTATCTGTGAATATTGCCGAGGAGCGTAATACAG
TTGAAGAGATGTATGAACCCTACTTAATTCAAAAAGGCTTTCTCATGCGAACACGAACAGGTCGTGTAGCGACGCAAAAG
GCTTATCGTCATCTTGGCTATCCCTATCAAAACACTTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

3D structure

• AlphaFold DB: A2RC05

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae R6	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae D39	84.848	99.398	0.843
ruvB	Streptococcus pneumoniae TIGR4	84.545	99.398	0.84
ruvB	Bacillus subtilis subsp. subtilis str. 168	58.824	97.289	0.572
ruvB	Helicobacter pylori 26695	48.936	99.096	0.485
ruvB	Synechocystis sp. PCC 6803	50.16	94.277	0.473