Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FPL03_RS17670 Genome accession   NZ_CP041963
Coordinates   3858843..3860579 (-) Length   578 a.a.
NCBI ID   WP_029828360.1    Uniprot ID   -
Organism   Xanthomonas citri pv. glycines strain 1157     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3853843..3865579
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FPL03_RS17645 (FPL03_17645) - 3854664..3856277 (+) 1614 WP_016850708.1 HAMP domain-containing sensor histidine kinase -
  FPL03_RS17650 (FPL03_17650) pilR 3856542..3857996 (+) 1455 WP_016850709.1 sigma-54 dependent transcriptional regulator Regulator
  FPL03_RS17670 (FPL03_17670) pilB 3858843..3860579 (-) 1737 WP_029828360.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FPL03_RS17675 (FPL03_17675) pilA/pilAI 3860714..3861121 (-) 408 WP_016849608.1 pilin Machinery gene
  FPL03_RS17680 (FPL03_17680) pilC 3861480..3862733 (+) 1254 WP_026006934.1 type II secretion system F family protein Machinery gene
  FPL03_RS17685 (FPL03_17685) - 3862740..3863603 (+) 864 WP_016849610.1 A24 family peptidase -
  FPL03_RS17690 (FPL03_17690) coaE 3863617..3864228 (+) 612 WP_016849611.1 dephospho-CoA kinase -
  FPL03_RS24825 - 3864623..3864889 (+) 267 WP_154666337.1 hypothetical protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62578.73 Da        Isoelectric Point: 5.3168

>NTDB_id=374932 FPL03_RS17670 WP_029828360.1 3858843..3860579(-) (pilB) [Xanthomonas citri pv. glycines strain 1157]
MSVMGTANLVGITGIARRLVQDGAIDESAARAAMDQAAAAKIPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSVFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGASLG
DDDEGMDNLDVSVGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGDAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=374932 FPL03_RS17670 WP_029828360.1 3858843..3860579(-) (pilB) [Xanthomonas citri pv. glycines strain 1157]
ATGAGTGTCATGGGAACGGCTAATCTGGTTGGTATCACTGGAATCGCTCGGCGTCTGGTTCAGGATGGCGCGATCGACGA
GTCCGCTGCCCGCGCTGCAATGGACCAGGCTGCGGCAGCCAAGATTCCCTTGCCGCAATGGTTCTCCGAGAAGAAGCTGG
TCACCGCATCGCAGCTCGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGCTGGACGTGTCGGTATTCGATGCCAGC
CAGAATGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGCACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAGCCGA
TCCTGGTCGACGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTGGGTGCGTCGCTCGGC
GACGACGACGAGGGGATGGACAACCTGGACGTGTCGGTCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACATTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCGATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGAATGGGGTGACTAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.172

100

0.554
  pilB Acinetobacter baylyi ADP1

55.634

98.27

0.547
  pilB Legionella pneumophila strain ERS1305867

52.373

98.443

0.516
  pilB Vibrio cholerae strain A1552

49.74

99.827

0.497
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio parahaemolyticus RIMD 2210633

46.809

97.578

0.457
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB/pilB1 Synechocystis sp. PCC 6803

36.946

100

0.389

Multiple sequence alignment