Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FOZ66_RS25815 Genome accession   NZ_CP041945
Coordinates   5490911..5492614 (+) Length   567 a.a.
NCBI ID   WP_003116249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain ST773     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 5472952..5490079 5490911..5492614 flank 832


Gene organization within MGE regions


Location: 5472952..5492614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FOZ66_RS25695 (FOZ66_26410) - 5472952..5474166 (+) 1215 WP_033938764.1 site-specific integrase -
  FOZ66_RS25700 (FOZ66_26415) - 5474163..5474693 (-) 531 WP_033938765.1 hypothetical protein -
  FOZ66_RS25705 (FOZ66_26420) - 5474815..5475015 (+) 201 WP_033989250.1 helix-turn-helix domain-containing protein -
  FOZ66_RS25710 (FOZ66_26425) - 5475012..5475428 (+) 417 WP_033938766.1 hypothetical protein -
  FOZ66_RS25715 (FOZ66_26430) - 5475421..5475654 (+) 234 WP_023110507.1 hypothetical protein -
  FOZ66_RS25720 (FOZ66_26435) - 5475651..5475920 (+) 270 WP_033938767.1 hypothetical protein -
  FOZ66_RS25725 (FOZ66_26440) - 5475917..5476219 (+) 303 WP_033938769.1 hypothetical protein -
  FOZ66_RS32590 - 5476222..5476659 (+) 438 WP_306283418.1 hypothetical protein -
  FOZ66_RS25735 (FOZ66_26450) - 5476698..5477165 (+) 468 WP_033938771.1 hypothetical protein -
  FOZ66_RS32660 - 5477172..5477777 (+) 606 WP_023110508.1 DUF3631 domain-containing protein -
  FOZ66_RS25745 (FOZ66_26460) - 5477833..5478729 (+) 897 WP_023107349.1 hypothetical protein -
  FOZ66_RS25750 (FOZ66_26465) - 5478860..5479273 (+) 414 WP_031629315.1 hypothetical protein -
  FOZ66_RS25755 (FOZ66_26470) - 5479424..5479999 (-) 576 WP_124101441.1 hypothetical protein -
  FOZ66_RS25760 (FOZ66_26475) - 5480776..5481282 (-) 507 WP_003096122.1 GNAT family N-acetyltransferase -
  FOZ66_RS25765 (FOZ66_26480) - 5481742..5481984 (-) 243 WP_003096120.1 hypothetical protein -
  FOZ66_RS25770 (FOZ66_26485) - 5482023..5482304 (+) 282 WP_015649789.1 hypothetical protein -
  FOZ66_RS25775 (FOZ66_26490) - 5482304..5482540 (+) 237 WP_003096116.1 hypothetical protein -
  FOZ66_RS25780 (FOZ66_26495) - 5482678..5482914 (-) 237 WP_071536284.1 DUF7336 domain-containing protein -
  FOZ66_RS25785 (FOZ66_26500) - 5482943..5485705 (-) 2763 WP_123186583.1 RHS repeat domain-containing protein -
  FOZ66_RS25790 (FOZ66_26505) - 5485720..5486868 (-) 1149 WP_023098865.1 DUF6531 domain-containing protein -
  FOZ66_RS25795 (FOZ66_26510) - 5488896..5489138 (+) 243 WP_031690159.1 hypothetical protein -
  FOZ66_RS25805 (FOZ66_26520) - 5489381..5490079 (-) 699 WP_223677410.1 hypothetical protein -
  FOZ66_RS25810 (FOZ66_26525) - 5490143..5490682 (-) 540 WP_033938465.1 pilin -
  FOZ66_RS25815 (FOZ66_26530) pilB 5490911..5492614 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62610.54 Da        Isoelectric Point: 5.5603

>NTDB_id=374842 FOZ66_RS25815 WP_003116249.1 5490911..5492614(+) (pilB) [Pseudomonas aeruginosa strain ST773]
MNDTIQLSGLARQLVLHELLDEKAAQQAQSQAQRNKLSLVTYLVQSKLVKGQALIELAADQFGIAYCDLNSLERDSLPKD
LISEKLVRQHRVVPLWRRGNKLFVGISDPANHQAINDVQFSTGLTTEAILVEDDKLGIIIEKLFENATDSLAGLDDVDLE
GLDVGSGAGASQDDDSSAETDDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKIYRVRFRTDGMLHEVAKPPIQLASRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHEVPKETLLHEGFP
EDRIGTFKLYSPVGCDHCKNGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=374842 FOZ66_RS25815 WP_003116249.1 5490911..5492614(+) (pilB) [Pseudomonas aeruginosa strain ST773]
ATGAACGACACAATTCAACTCAGCGGCTTGGCTCGCCAGCTCGTCCTGCATGAACTTCTCGATGAAAAGGCCGCCCAACA
GGCCCAATCGCAGGCCCAGCGCAACAAGTTGTCGCTGGTAACCTATCTGGTACAAAGCAAGCTAGTGAAAGGGCAGGCCT
TGATCGAACTGGCTGCGGATCAATTCGGTATCGCCTACTGCGACCTGAACAGCCTTGAGCGGGACAGTCTGCCCAAGGAT
CTGATCAGCGAGAAGCTGGTGCGCCAGCATCGAGTAGTTCCGTTGTGGCGACGCGGCAACAAGCTGTTCGTCGGCATCTC
CGATCCAGCCAACCACCAGGCGATTAACGATGTGCAGTTCAGCACAGGCCTGACTACTGAAGCCATCCTGGTCGAGGATG
ACAAGCTCGGCATCATCATCGAGAAACTATTCGAAAATGCCACTGACAGCCTTGCAGGACTGGACGACGTGGATCTGGAA
GGCCTGGATGTCGGTTCCGGTGCGGGCGCAAGCCAGGATGATGATTCCAGTGCAGAGACAGACGACGCCCCGGTGGTGCG
TTTCGTCAACAAAATGTTGTTGGATGCGATCAGAGGCGGCTCCTCCGACCTGCATTTCGAGCCCTATGAAAAGATTTACC
GCGTACGTTTCCGCACCGATGGCATGCTCCATGAAGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGTATTTCCGCGCGA
CTCAAGGTGATGGCCGGCCTGGATATCTCCGAACGGCGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAC
CAAGTCCATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGTATCCTCGATTCCTCCA
GCGCGCAGATGGGCATCGACGCCCTCGGCTACGAGGAAGACCAGAAGGAACTCTACCTGGCTGCGCTCAAGCAGCCGCAG
GGCATGATCCTGGTCACCGGGCCCACCGGCTCGGGCAAGACGGTCTCCCTGTACACCGGCCTGAACATCCTCAACACCAC
CGACATCAACATCTCCACTGCCGAAGACCCGGTGGAGATCAACTTGGAAGGCATCAACCAGGTCAACGTCAATCCGCGCC
AGGGCATGGACTTCTCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATTCGCGAC
CTGGAGACGGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
CGAGACCCTGACCCGCCTGCTGAACATGGGCGTGCCAGCGTTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGC
GACTTGCGCGAAAACTCTGCTCGCACTGCAAGAAAGAGCATGAGGTACCGAAGGAAACACTCCTCCATGAAGGCTTCCCC
GAGGATCGGATCGGCACCTTCAAGCTGTATTCACCAGTAGGCTGCGACCATTGCAAGAACGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACCCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGAGATCGCCGAGC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGGATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.895

100

0.582
  pilB Acinetobacter baylyi ADP1

57.27

99.471

0.57
  pilB Legionella pneumophila strain ERS1305867

55.106

100

0.552
  pilF Neisseria gonorrhoeae MS11

50.444

99.295

0.501
  pilB Vibrio parahaemolyticus RIMD 2210633

51.948

95.062

0.494
  pilB Vibrio cholerae strain A1552

50.182

97.002

0.487
  pilB Vibrio campbellii strain DS40M4

50.832

95.414

0.485
  pilF Thermus thermophilus HB27

38.584

99.647

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.189

93.474

0.376

Multiple sequence alignment