Detailed information NTG0373762

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FFH21_RS12345
• Genome accession: NZ_CP041754
• Coordinates: 2612144..2613313 (+)
• Length: 389 a.a.
• NCBI ID: WP_011168499.1
• Uniprot ID: Q48JA6
• Organism: Pseudomonas sp. KBS0707
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2607144..2618313

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FFH21_RS12330 (FFH21_012330)	-	2607581..2609908 (+)	2328	WP_019741978.1	ATP-dependent DNA helicase	-
FFH21_RS12335 (FFH21_012335)	pgm	2610024..2611670 (+)	1647	WP_011168498.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
FFH21_RS12340 (FFH21_012340)	-	2611745..2612020 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
FFH21_RS12345 (FFH21_012345)	pilU	2612144..2613313 (+)	1170	WP_011168499.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FFH21_RS12350 (FFH21_012350)	-	2613362..2614939 (-)	1578	WP_011168500.1	ABC transporter ATP-binding protein	-
FFH21_RS12355 (FFH21_012355)	-	2614941..2615963 (-)	1023	WP_011168501.1	ABC transporter permease	-
FFH21_RS12360 (FFH21_012360)	-	2615963..2617027 (-)	1065	WP_011168502.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42552.70 Da
• Isoelectric Point: 6.0634

>NTDB_id=373762 FFH21_RS12345 WP_011168499.1 2612144..2613313(+) (pilU) [Pseudomonas sp. KBS0707]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMDKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=373762 FFH21_RS12345 WP_011168499.1 2612144..2613313(+) (pilU) [Pseudomonas sp. KBS0707]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCCATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGATCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGATAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTTAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: Q48JA6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362