Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FFH90_RS26320 Genome accession   NZ_CP041753
Coordinates   5724827..5726527 (+) Length   566 a.a.
NCBI ID   WP_064620392.1    Uniprot ID   A0A2W7JGQ2
Organism   Pseudomonas sp. ATCC 43928     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5719827..5731527
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFH90_RS26300 (FFH90_026300) - 5720041..5723037 (+) 2997 WP_178114258.1 DUF748 domain-containing protein -
  FFH90_RS26305 (FFH90_026305) - 5723037..5723333 (+) 297 WP_064620386.1 DUF2845 domain-containing protein -
  FFH90_RS26310 (FFH90_026310) - 5723507..5723863 (-) 357 WP_064620389.1 BON domain-containing protein -
  FFH90_RS26315 (FFH90_026315) - 5724190..5724600 (-) 411 WP_019650990.1 pilin -
  FFH90_RS26320 (FFH90_026320) pilB 5724827..5726527 (+) 1701 WP_064620392.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FFH90_RS26325 (FFH90_026325) pilC 5726530..5727747 (+) 1218 WP_018930188.1 type II secretion system F family protein Machinery gene
  FFH90_RS26330 (FFH90_026330) pilD 5727749..5728621 (+) 873 WP_064620394.1 A24 family peptidase Machinery gene
  FFH90_RS26335 (FFH90_026335) coaE 5728618..5729241 (+) 624 WP_026286732.1 dephospho-CoA kinase -
  FFH90_RS26340 (FFH90_026340) yacG 5729238..5729438 (+) 201 WP_103406792.1 DNA gyrase inhibitor YacG -
  FFH90_RS26345 (FFH90_026345) - 5729448..5729663 (-) 216 WP_111454251.1 hypothetical protein -
  FFH90_RS26350 (FFH90_026350) - 5729727..5730416 (-) 690 WP_138866321.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62956.98 Da        Isoelectric Point: 5.6938

>NTDB_id=373735 FFH90_RS26320 WP_064620392.1 5724827..5726527(+) (pilB) [Pseudomonas sp. ATCC 43928]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRDRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLEKDTQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPSNQQVINDIQFSTGLNTEAILVEEDKLSDAIERFFDSHATDLQEMADVDLDG
LDIESVDDQKQDAIGGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKTYRVRMRTDGILHEVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVNINISTAEDPVEINMEGVNQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCNQCNAGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=373735 FFH90_RS26320 WP_064620392.1 5724827..5726527(+) (pilB) [Pseudomonas sp. ATCC 43928]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
GTATCAGCAAGCCCAACGCGATCGAGTCTCACTGGTCAGTTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGAAAAAGACACCCAACCCAAGGGGCTG
GTCAGCGAAAAACTGATCCGCCAGCACCACGCCCTACCCCTCTGGCGACGCGGCAACAAACTGTTCGTGGGGGTTTCCGA
TCCGAGTAATCAACAGGTCATCAACGACATCCAATTCAGTACCGGGCTGAACACCGAAGCCATCCTGGTCGAAGAGGACA
AGCTTAGCGACGCCATCGAAAGATTCTTCGACAGCCACGCCACCGACCTGCAAGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGTCGACGACCAGAAACAGGACGCCATCGGCGGACAGGACACCGATGACGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGGTCTTCCGACCTGCATTTCGAACCCTACGAAAAAACCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAAGTTGCCAAGCCACCAACGCAACTGGCCAACCGCATTGCTTCCCGGCTC
AAAGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGAATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTCGGCTATGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCACAGGGG
ATGATCCTGGTGACCGGGCCCACCGGTTCAGGCAAGACCGTGTCGCTCTATACCGGGCTGAACATCCTCAACACCGTCAA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAGGGCGTCAACCAGGTCAACGTCAATCCCAGGCAGG
GAATGGATTTCGCCCAGGCGTTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACTGCCGAAATTGCCATCAAGGCCGCCCAGACCGGGCACATGGTGCTTTCCACCCTGCACACCAACAGCGCTGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGCTTCAACATTGCGACCTCGGTCAGCCTGATCATTGCCCAGCGCC
TGGCGCGCAAGTTGTGCAGCCATTGCAAGAAGCCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCATTCACGATCTATGAACCGGTCGGTTGCAATCAATGCAATGCTGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTGAAGAACACTCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAGATCGACCTCCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACGTCGGGCTTGCTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7JGQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.876

99.823

0.578
  pilB Legionella pneumophila strain ERS1305867

56.007

100

0.56
  pilB Vibrio cholerae strain A1552

50.617

100

0.507
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilB Vibrio parahaemolyticus RIMD 2210633

49.64

98.233

0.488
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilF Thermus thermophilus HB27

36.572

100

0.366

Multiple sequence alignment