Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   FFH90_RS03025 Genome accession   NZ_CP041753
Coordinates   660764..661828 (+) Length   354 a.a.
NCBI ID   WP_018928618.1    Uniprot ID   A0A2W7JGQ6
Organism   Pseudomonas sp. ATCC 43928     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 655764..666828
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FFH90_RS03015 (FFH90_003015) - 656706..657974 (+) 1269 WP_018929144.1 malic enzyme-like NAD(P)-binding protein -
  FFH90_RS03020 (FFH90_003020) - 658108..660549 (-) 2442 WP_111453922.1 penicillin-binding protein 1A -
  FFH90_RS03025 (FFH90_003025) comM 660764..661828 (+) 1065 WP_018928618.1 pilus assembly protein PilM Machinery gene
  FFH90_RS03030 (FFH90_003030) - 661828..662394 (+) 567 WP_138866666.1 PilN domain-containing protein -
  FFH90_RS03035 (FFH90_003035) pilO 662391..663014 (+) 624 WP_018928616.1 type 4a pilus biogenesis protein PilO -
  FFH90_RS03040 (FFH90_003040) - 663011..663538 (+) 528 WP_138866667.1 pilus assembly protein PilP -
  FFH90_RS03045 (FFH90_003045) pilQ 663552..665627 (+) 2076 WP_064615925.1 type IV pilus secretin PilQ Machinery gene
  FFH90_RS03050 (FFH90_003050) aroK 665632..666150 (+) 519 WP_019581860.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38080.46 Da        Isoelectric Point: 4.6675

>NTDB_id=373721 FFH90_RS03025 WP_018928618.1 660764..661828(+) (comM) [Pseudomonas sp. ATCC 43928]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALARVLLKAKTSQRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGASVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQEHLTVAVVDIGATMTTLSVLNNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYSSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMTLSSRVNASALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=373721 FFH90_RS03025 WP_018928618.1 660764..661828(+) (comM) [Pseudomonas sp. ATCC 43928]
GTGCTAGGACTCTTCAATAAAAAAACCAATGCGCTTCTGGGGATCGACATCAGCTCTACGTCGGTGAAGCTGTTGGAGTT
AAGCCGCCAAGGCGACCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCGCTGCCCGCCAGTGCCGTGGTCGAAAAAAATA
TTGCCGAGCTCGAAGGCGTGGGGCAGGCCCTTGCGCGCGTGCTGCTCAAGGCCAAAACCAGCCAGAGGAACGTCGCCGTG
GCGGTCGCCGGCTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCGTATCCGCTGGATGAAGTGGCGATCGACTTTGAAGTGCAAGGCGCGTCAGTGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTTCGTGAAGCGGCCCTGGCCCTG
GCCGGGCTGACCGCGCGAGTAGTCGATGTCGAAGCCTATGCCCTGGAGCGAGCGTTCGGCTTGCTGGCAACCCAGCTGGC
CGCCTCTCAGGAGCATCTGACCGTGGCGGTAGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGTTGAATAATGGGC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGCCGACAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGACGGTC
GAGCAGGCTGGGTTGGCGAAGAAACAGGGTGGTTTGCCGGACGATTACGTCAGTGAGGTCTTGCAGCCGTTTCGGGATGC
GTTGGTGCAACAAGTATCGCGCTCATTGCAGTTTTTCTTTGCCTCCGGCCAATACAGCTCGGTCGATCACATTCTCCTGG
CCGGCGGCACTGCCTCGGTCCCAGGGCTGGATCGGTTGATCGAGCAGCGTCTGGGTACGCCGACCCAGGTCGCTAACCCA
TTCTCCGACATGACCCTGAGCAGCAGGGTCAACGCCAGTGCCCTGGCGAGCGATGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7JGQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment