Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   G6Z99_RS03695 Genome accession   NZ_CP049201
Coordinates   768784..770277 (+) Length   497 a.a.
NCBI ID   WP_115204385.1    Uniprot ID   -
Organism   Escherichia coli strain PapRG-04-4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 763784..775277
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G6Z99_RS03675 (G6Z99_03670) pilD 764432..765241 (+) 810 WP_096840167.1 prepilin peptidase PppA Machinery gene
  G6Z99_RS03680 (G6Z99_03675) gspS2 765307..765717 (+) 411 WP_021557202.1 type II secretion system pilot lipoprotein GspS-beta -
  G6Z99_RS03685 (G6Z99_03680) gspC 765735..766694 (+) 960 WP_097470220.1 type II secretion system protein GspC -
  G6Z99_RS03690 (G6Z99_03685) gspD 766724..768784 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  G6Z99_RS03695 (G6Z99_03690) pilB/pilB1 768784..770277 (+) 1494 WP_115204385.1 type II secretion system ATPase GspE Machinery gene
  G6Z99_RS03700 (G6Z99_03695) gspF 770277..771500 (+) 1224 WP_164475437.1 type II secretion system inner membrane protein GspF -
  G6Z99_RS03705 (G6Z99_03700) gspG 771517..771972 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  G6Z99_RS03710 (G6Z99_03705) gspH 771976..772539 (+) 564 WP_164475438.1 type II secretion system minor pseudopilin GspH -
  G6Z99_RS03715 (G6Z99_03710) gspI 772536..772907 (+) 372 WP_001718860.1 type II secretion system minor pseudopilin GspI -
  G6Z99_RS03720 (G6Z99_03715) gspJ 772904..773509 (+) 606 WP_069913163.1 type II secretion system minor pseudopilin GspJ -
  G6Z99_RS03725 (G6Z99_03720) gspK 773506..774483 (+) 978 WP_069913164.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55120.28 Da        Isoelectric Point: 6.1631

>NTDB_id=373162 G6Z99_RS03695 WP_115204385.1 768784..770277(+) (pilB/pilB1) [Escherichia coli strain PapRG-04-4]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQALNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=373162 G6Z99_RS03695 WP_115204385.1 768784..770277(+) (pilB/pilB1) [Escherichia coli strain PapRG-04-4]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGCGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACAGGTGTGATGGCGCAGCGGCTGGTTCGGACGCTGTGTCCCGACTGCCGTCAGGCCGCGCCTGCG
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCACAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment