Detailed information NTG0372777

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: WPAWT_RS26165
• Genome accession: NZ_CP049161
• Coordinates: 5689149..5690213 (-)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain MS14403
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5684149..5695213

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WPAWT_RS26140 (WPAWT_05220)	aroK	5684696..5685214 (-)	519	WP_003095833.1	shikimate kinase AroK	-
WPAWT_RS26145 (WPAWT_05221)	pilQ	5685226..5687358 (-)	2133	WP_003116137.1	type 4a pilus secretin PilQ	Machinery gene
WPAWT_RS26150 (WPAWT_05222)	comL/pilP	5687412..5687936 (-)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
WPAWT_RS26155 (WPAWT_05223)	pilO	5687933..5688556 (-)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	Machinery gene
WPAWT_RS26160 (WPAWT_05224)	pilN	5688553..5689149 (-)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
WPAWT_RS26165 (WPAWT_05225)	pilM	5689149..5690213 (-)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
WPAWT_RS26170 (WPAWT_05226)	-	5690398..5692869 (+)	2472	WP_171978696.1	penicillin-binding protein 1A	-
WPAWT_RS26175 (WPAWT_05227)	-	5692974..5694242 (-)	1269	WP_003103278.1	malic enzyme	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=372777 WPAWT_RS26165 WP_003110888.1 5689149..5690213(-) (pilM) [Pseudomonas aeruginosa strain MS14403]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=372777 WPAWT_RS26165 WP_003110888.1 5689149..5690213(-) (pilM) [Pseudomonas aeruginosa strain MS14403]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466