Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FLM54_RS13365 Genome accession   NZ_CP041327
Coordinates   2894705..2896381 (+) Length   558 a.a.
NCBI ID   WP_178956622.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. Scap25     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2889705..2901381
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLM54_RS13340 (FLM54_13405) nadC 2889728..2890573 (+) 846 WP_178956617.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FLM54_RS13345 (FLM54_13410) pilA2 2891110..2891550 (+) 441 WP_178956618.1 pilin Machinery gene
  FLM54_RS13350 (FLM54_13415) pilC 2891615..2892838 (+) 1224 WP_178956619.1 type II secretion system F family protein Machinery gene
  FLM54_RS13355 (FLM54_13420) pilD 2893026..2893937 (+) 912 WP_178956620.1 prepilin peptidase Machinery gene
  FLM54_RS13360 (FLM54_13425) coaE 2893940..2894569 (+) 630 WP_178956621.1 dephospho-CoA kinase -
  FLM54_RS13365 (FLM54_13430) pilB 2894705..2896381 (+) 1677 WP_178956622.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FLM54_RS13370 (FLM54_13435) yacG 2896447..2896677 (+) 231 WP_054202591.1 DNA gyrase inhibitor YacG -
  FLM54_RS13375 (FLM54_13440) - 2896737..2897225 (-) 489 WP_138605552.1 periplasmic heavy metal sensor -
  FLM54_RS13380 (FLM54_13445) - 2897392..2898081 (+) 690 WP_178956623.1 response regulator -
  FLM54_RS13385 (FLM54_13450) - 2898059..2899486 (+) 1428 WP_178956624.1 ATP-binding protein -
  FLM54_RS13390 (FLM54_13455) - 2899476..2900420 (+) 945 WP_178956625.1 alpha/beta fold hydrolase -
  FLM54_RS13395 (FLM54_13460) - 2900468..2901298 (-) 831 WP_178956870.1 cation diffusion facilitator family transporter -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62283.61 Da        Isoelectric Point: 5.7838

>NTDB_id=371208 FLM54_RS13365 WP_178956622.1 2894705..2896381(+) (pilB) [Pseudoalteromonas sp. Scap25]
MNINSPLLRKFITLGRVDTDTVKARQQDFASTAELISKCGKIDSNDLAEQCIDLFRVPFFDLKDFNVAQIPAELVKEKLI
RKHHILPLVQKGRKVYIAASDPTDYGAFENFEFSTGLSCEVVVVDYIQLDNKIEQLLDAAGSLNLSEDEFKEFAELDSEP
QKDHTPKDDDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHKYRIRFRIDGILHEVASPPNTLASKLSARIKVMSRLDI
AEKRKPQDGRIKLKVTERKSIDFRVSTMPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYLDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGVNQVQINIKADMTFANALRAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETITRLLNMGVPAYNVASSISLIIAQRLARRLCSKCKTPEQLPREELIRQGFSEQQIQDMVL
YAPKGCESCTEGYKGRVGIYEVMKITPEIAQIIMRGGNSLEIAEVSLKAGYNNLRLSGIRKAAEGVTSLAEINRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=371208 FLM54_RS13365 WP_178956622.1 2894705..2896381(+) (pilB) [Pseudoalteromonas sp. Scap25]
ATGAATATTAACTCACCGTTACTAAGAAAATTTATTACCCTCGGCCGTGTCGATACCGATACCGTAAAAGCCAGGCAGCA
AGACTTTGCGTCAACCGCAGAACTTATTTCTAAATGCGGAAAAATAGATAGCAACGATTTAGCTGAGCAATGTATTGATT
TGTTTAGAGTGCCTTTTTTTGATTTAAAAGATTTTAATGTTGCACAAATTCCTGCCGAGCTAGTTAAAGAAAAGCTGATC
CGTAAGCATCATATTTTACCTTTGGTACAAAAAGGCAGAAAAGTATATATTGCCGCTTCAGATCCTACCGATTACGGCGC
TTTTGAAAACTTTGAATTTAGCACCGGTTTATCGTGTGAAGTGGTGGTAGTTGACTACATTCAACTCGATAATAAAATTG
AGCAACTACTGGATGCTGCAGGTAGCTTAAATTTAAGTGAGGATGAATTTAAAGAGTTTGCTGAACTAGATTCTGAGCCA
CAAAAAGACCATACGCCAAAAGACGATGACAAAGACGACGCCCCTATTATTGTCTACATTAATAAAATTTTAATGGACGC
AATAAAAAAAGGCGCTTCGGATCTTCACTTTGAACCTTATGAACATAAATACCGTATTCGCTTTCGTATCGACGGAATTT
TACATGAGGTAGCCAGCCCGCCAAACACCTTGGCCAGTAAGCTATCTGCCCGTATAAAAGTTATGTCGCGCCTTGATATC
GCTGAAAAACGTAAGCCACAAGATGGCCGTATAAAACTCAAAGTGACAGAACGTAAAAGTATTGATTTTCGTGTTAGTAC
CATGCCTACCTTATGGGGCGAAAAAATTGTAATGCGTATCCTCGATTCATCAAGTGCCATGTTAGGTATTGATGTATTAG
GTTATGAACCTGAGCAGAAAAAACTCTACCTCGATGCATTAGAGCAACCACAAGGGATGATTTTAGTTACCGGTCCAACT
GGCTCAGGTAAAACCGTCTCCCTGTATACGGGTTTAAATATACTCAATCAGCCAGAGCGTAACATCAGTACCGCCGAAGA
TCCGGTAGAAATTAACCTCGAAGGGGTTAATCAGGTACAAATAAACATTAAAGCCGACATGACCTTTGCTAATGCTTTAC
GAGCGTTCTTACGCCAAGATCCCGATGTAGTAATGGTAGGTGAGATACGAGATTTAGAAACCGCTGAAATATCAATTAAA
GCGGCACAAACCGGTCACTTAGTACTATCAACGCTACATACTAACTCTGCACCTGAAACCATAACTCGTTTATTAAACAT
GGGCGTACCCGCGTATAATGTGGCCAGTTCAATTAGCTTAATAATCGCTCAGCGATTAGCGCGCCGATTATGCTCTAAAT
GTAAAACACCAGAGCAGTTACCCCGTGAAGAGCTTATTCGCCAAGGCTTTAGTGAACAACAAATACAGGATATGGTTTTG
TATGCCCCTAAAGGCTGTGAAAGCTGTACTGAGGGCTATAAAGGCCGTGTTGGTATTTACGAGGTTATGAAAATCACCCC
CGAAATAGCGCAAATTATAATGCGTGGTGGTAACTCATTAGAAATTGCTGAGGTATCACTTAAAGCAGGGTATAACAATT
TGCGTTTATCAGGTATTCGTAAAGCAGCAGAGGGTGTGACCTCGCTGGCTGAGATAAACCGCGTTACTAGTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(182-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.778

96.774

0.559
  pilB Acinetobacter baylyi ADP1

58.812

90.502

0.532
  pilB Legionella pneumophila strain ERS1305867

55.796

91.219

0.509
  pilB Vibrio parahaemolyticus RIMD 2210633

52.82

95.341

0.504
  pilB Vibrio cholerae strain A1552

52.523

95.878

0.504
  pilB Vibrio campbellii strain DS40M4

51.124

95.699

0.489
  pilF Neisseria gonorrhoeae MS11

49.425

93.548

0.462

Multiple sequence alignment