Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   G3M72_RS17975 Genome accession   NZ_CP048604
Coordinates   3775548..3777041 (+) Length   497 a.a.
NCBI ID   WP_163388300.1    Uniprot ID   -
Organism   Escherichia coli strain PapRG-06-3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3770548..3782041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M72_RS17955 (G3M72_17955) pilD 3771196..3772005 (+) 810 WP_001345955.1 prepilin peptidase PppA Machinery gene
  G3M72_RS17960 (G3M72_17960) gspS2 3772071..3772481 (+) 411 WP_001345954.1 type II secretion system pilot lipoprotein GspS-beta -
  G3M72_RS17965 (G3M72_17965) gspC 3772499..3773458 (+) 960 WP_163388299.1 type II secretion system protein GspC -
  G3M72_RS17970 (G3M72_17970) gspD 3773488..3775548 (+) 2061 WP_000498817.1 type II secretion system secretin GspD -
  G3M72_RS17975 (G3M72_17975) pilB/pilB1 3775548..3777041 (+) 1494 WP_163388300.1 type II secretion system ATPase GspE Machinery gene
  G3M72_RS17980 (G3M72_17980) gspF 3777041..3778264 (+) 1224 WP_001173453.1 type II secretion system inner membrane protein GspF -
  G3M72_RS17985 (G3M72_17985) gspG 3778281..3778736 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  G3M72_RS17990 (G3M72_17990) gspH 3778740..3779303 (+) 564 WP_001115140.1 type II secretion system minor pseudopilin GspH -
  G3M72_RS17995 (G3M72_17995) gspI 3779300..3779671 (+) 372 WP_000820125.1 type II secretion system minor pseudopilin GspI -
  G3M72_RS18000 (G3M72_18000) gspJ 3779668..3780267 (+) 600 WP_001255040.1 type II secretion system minor pseudopilin GspJ -
  G3M72_RS18005 (G3M72_18005) gspK 3780270..3781247 (+) 978 WP_000633238.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55071.24 Da        Isoelectric Point: 6.0377

>NTDB_id=369904 G3M72_RS17975 WP_163388300.1 3775548..3777041(+) (pilB/pilB1) [Escherichia coli strain PapRG-06-3]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITGACTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELEMERYVRQHSAGVRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=369904 G3M72_RS17975 WP_163388300.1 3775548..3777041(+) (pilB/pilB1) [Escherichia coli strain PapRG-06-3]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCGGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTGAGCCAGCAACTGACC
GCGCAGCTGCGCCAGTTACTGCACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAATCACTCGCGTAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAT
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCGTCATTGACCGGACACCT
GGTGCTTTCCACGCTGCATACCAATACGGCGGTGGGGGCGATCACGCGTCTGCAGGATATGGGCGTGGAGCCTTTTCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAACGACTGGTCCGCACGCTGTGCCCCGACTGCCGCCAGGCTGCGCCCGCC
ACCGACGAGGAAAAACGCCTGCTGGGGATTACCGGTGCGTGTACCGTCACGCTGTACCATCCGCAGGGATGCCCCGCCTG
TAATCACAAAGGCTTTCGCGGACGTACTGCCATCCATGAGCTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGATGGAACGTTATGTCCGGCAACACTCTGCGGGGGTCCGCAGCAACGGTATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTTGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.196

86.117

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Legionella pneumophila strain ERS1305867

43.155

86.72

0.374
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362

Multiple sequence alignment