Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G3M71_RS01935 Genome accession   NZ_CP048603
Coordinates   424500..425981 (-) Length   493 a.a.
NCBI ID   WP_163388376.1    Uniprot ID   -
Organism   Escherichia coli strain ChlosBP-23-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419500..430981
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G3M71_RS01905 (G3M71_01905) gspK 420413..421396 (-) 984 WP_089635986.1 type II secretion system minor pseudopilin GspK -
  G3M71_RS01910 (G3M71_01910) gspJ 421389..421976 (-) 588 WP_163388375.1 type II secretion system minor pseudopilin GspJ -
  G3M71_RS01915 (G3M71_01915) gspI 421969..422346 (-) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  G3M71_RS01920 (G3M71_01920) gspH 422343..422852 (-) 510 Protein_387 type II secretion system minor pseudopilin GspH -
  G3M71_RS01925 (G3M71_01925) gspG 422860..423297 (-) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  G3M71_RS01930 (G3M71_01930) gspF 423307..424503 (-) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  G3M71_RS01935 (G3M71_01935) pilB 424500..425981 (-) 1482 WP_163388376.1 type II secretion system ATPase GspE Machinery gene
  G3M71_RS01940 (G3M71_01940) gspD 425991..427943 (-) 1953 WP_001525807.1 type II secretion system secretin GspD -
  G3M71_RS01945 (G3M71_01945) gspC 427927..428742 (-) 816 WP_001142674.1 type II secretion system protein GspC -
  G3M71_RS01950 (G3M71_01950) gspA 428922..430391 (+) 1470 WP_001683343.1 type II secretion system protein GspA -
  G3M71_RS01955 (G3M71_01955) gspB 430393..430812 (+) 420 WP_000461443.1 putative general secretion pathway protein GspB -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54752.73 Da        Isoelectric Point: 6.2412

>NTDB_id=369766 G3M71_RS01935 WP_163388376.1 424500..425981(-) (pilB) [Escherichia coli strain ChlosBP-23-1]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQFVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=369766 G3M71_RS01935 WP_163388376.1 424500..425981(-) (pilB) [Escherichia coli strain ChlosBP-23-1]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATTCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAGATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCCCAGCAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTACCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.662

88.844

0.406
  pilB Legionella pneumophila strain ERS1305867

41.164

97.566

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.416

94.523

0.391
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361