Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FIV34_RS05995 Genome accession   NZ_CP041046
Coordinates   1311345..1313072 (-) Length   575 a.a.
NCBI ID   WP_139980638.1    Uniprot ID   A0A4Y5Z115
Organism   Luteibacter pinisoli strain MAH-14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1306345..1318072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FIV34_RS05975 (FIV34_05975) - 1307120..1308373 (+) 1254 WP_139980630.1 GGDEF domain-containing phosphodiesterase -
  FIV34_RS05980 (FIV34_05980) coaE 1308387..1308989 (-) 603 WP_139980632.1 dephospho-CoA kinase -
  FIV34_RS05985 (FIV34_05985) - 1308986..1309855 (-) 870 WP_139980634.1 A24 family peptidase -
  FIV34_RS05990 (FIV34_05990) pilC 1309938..1311203 (-) 1266 WP_139980636.1 type II secretion system F family protein Machinery gene
  FIV34_RS05995 (FIV34_05995) pilB 1311345..1313072 (-) 1728 WP_139980638.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FIV34_RS06000 (FIV34_06000) - 1313222..1314094 (+) 873 WP_139985734.1 glycosyltransferase family 2 protein -
  FIV34_RS06005 (FIV34_06005) - 1314091..1314819 (+) 729 WP_139980640.1 class I SAM-dependent methyltransferase -
  FIV34_RS06010 (FIV34_06010) - 1314878..1315969 (+) 1092 WP_170207522.1 glycosyltransferase family 4 protein -
  FIV34_RS06015 (FIV34_06015) - 1315969..1316895 (+) 927 WP_139980644.1 glycosyltransferase -
  FIV34_RS06020 (FIV34_06020) - 1316892..1317938 (+) 1047 WP_139980646.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62576.34 Da        Isoelectric Point: 6.5853

>NTDB_id=369159 FIV34_RS05995 WP_139980638.1 1311345..1313072(-) (pilB) [Luteibacter pinisoli strain MAH-14]
MATQMHQPTLAGLTGMARRLVTEGVLGEAEVRKAVQDSAEKRVSLSAWLVDHHLVDSPRLSQVASAEFGMPLMDIGNMLP
ANMPLDLVTEALITKHQALPLFKRGKRLFVGIADPMQSHALDEIKFHSNHMVEPVLVERGQLRRIIDSALAAANNNVPAF
DGGGLDDLVLDAGPDEGDGTTGIDANANDDAPIVKFVNKILVDAIKRGASDIHFEPFETVYRVRLRMDGILRIVASAPIK
LGTRIASRLKVMSGLDIAERRVPQDGRIKLNLTKTRAIDFRVSTLPTLFGEKIVLRVLDGSSAKLGIDKLGYEEIQKQLY
LDAIEKPYGMVLVTGPTGSGKTVSLYTALNILNTEGRNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNIISSVTLIIAQRLARRLHDCKREISLPPAA
LLAEGFTQEEVDAGITVYEAVGCDSCNEGYKGRLGIYQVMPMLEDIQKIVLEGGNAMQIAEVAKKAGVNDLRASALLKVR
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=369159 FIV34_RS05995 WP_139980638.1 1311345..1313072(-) (pilB) [Luteibacter pinisoli strain MAH-14]
ATGGCGACACAGATGCACCAGCCCACGCTCGCGGGCCTGACCGGCATGGCCAGGCGTCTTGTGACCGAAGGCGTCCTTGG
CGAGGCGGAAGTCCGCAAGGCCGTGCAGGATTCCGCCGAGAAGCGCGTGTCGCTGTCGGCATGGCTGGTGGACCACCATC
TGGTTGATAGCCCCCGCCTCTCCCAGGTGGCGTCGGCCGAGTTCGGCATGCCCCTGATGGACATCGGCAACATGTTGCCC
GCCAACATGCCGCTGGACCTGGTCACCGAAGCCCTCATCACCAAGCATCAGGCGCTGCCCCTGTTCAAGCGTGGCAAGCG
CCTGTTCGTCGGCATCGCCGACCCGATGCAGTCGCACGCCCTGGACGAGATCAAGTTCCACTCCAACCACATGGTGGAGC
CGGTGCTGGTCGAGCGCGGGCAGCTTCGTCGCATCATTGATTCGGCGCTCGCCGCCGCCAACAACAACGTGCCCGCGTTC
GACGGTGGAGGCCTCGACGACCTTGTCCTCGATGCGGGCCCCGACGAGGGCGACGGCACCACCGGCATCGACGCGAACGC
CAACGACGACGCGCCTATCGTCAAGTTCGTCAACAAGATCCTGGTCGACGCGATCAAGCGCGGCGCCTCGGATATCCATT
TCGAGCCCTTTGAAACCGTCTACCGCGTCCGCCTGCGCATGGACGGCATCCTGCGCATCGTCGCCTCGGCACCGATCAAG
CTGGGCACCCGCATCGCCTCGCGCCTGAAGGTGATGAGCGGCCTGGATATCGCCGAGCGCCGCGTGCCGCAGGATGGCCG
CATCAAGCTCAACCTGACCAAGACCCGCGCCATCGACTTCCGCGTCAGCACCCTGCCCACCCTTTTCGGTGAAAAGATCG
TGCTGCGCGTGCTGGATGGCTCCTCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAAGAGATCCAGAAGCAGCTCTAC
CTCGACGCCATCGAGAAGCCCTATGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGTAAGACGGTGTCGCTGTATAC
GGCCCTGAACATCCTGAATACCGAGGGGCGCAACATCTCCACGGTGGAAGACCCGGTCGAAATCCGCGTCGAGGGCATCA
ACCAGGTCCAGCAGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCCCTGCGCTCCTTCCTGCGCCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAAATCGCGGTCAAGGCGGCGCAGACGGGCCATATGGTCCTGTC
CACCCTGCATACCAACGACGCCCCGCAGACCATCGCCCGACTGATGAACATGGGCATCGCGCCCTACAACATCATTTCCT
CGGTCACCCTGATCATCGCCCAGCGCCTGGCCCGCCGCCTGCACGACTGCAAGCGCGAGATCAGCCTGCCGCCGGCCGCC
CTGCTCGCCGAGGGCTTCACCCAGGAAGAGGTGGATGCCGGCATCACGGTCTATGAGGCCGTCGGCTGCGACAGCTGCAA
CGAGGGCTACAAGGGGCGCCTAGGTATCTACCAGGTCATGCCTATGCTCGAAGACATCCAGAAGATCGTCCTGGAGGGGG
GCAACGCCATGCAGATCGCGGAAGTGGCGAAGAAGGCGGGGGTCAACGACCTTCGCGCGTCGGCCCTGCTCAAGGTCAGG
AACGGGGTCACCAGCCTCGCCGAAATCAACCGAGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A4Y5Z115

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.45

99.652

0.543
  pilB Acinetobacter baylyi ADP1

54.24

98.435

0.534
  pilB Legionella pneumophila strain ERS1305867

50.174

99.826

0.501
  pilB Vibrio cholerae strain A1552

48.932

97.739

0.478
  pilF Neisseria gonorrhoeae MS11

47.527

98.435

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

47.069

97.913

0.461
  pilB Vibrio campbellii strain DS40M4

46.643

98.435

0.459
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.455

100

0.379

Multiple sequence alignment