Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   GV831_RS00145 Genome accession   NZ_CP047891
Coordinates   23256..25139 (+) Length   627 a.a.
NCBI ID   WP_003130801.1    Uniprot ID   Q9CEF5
Organism   Lactococcus lactis subsp. lactis strain WHH2311     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 18256..30139
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GV831_RS00115 - 18466..18627 (+) 162 WP_014570719.1 hypothetical protein -
  GV831_RS00120 (GV831_00110) - 18742..19092 (+) 351 WP_014570718.1 hypothetical protein -
  GV831_RS00125 (GV831_00115) - 19105..19392 (+) 288 WP_014570717.1 phage holin -
  GV831_RS00130 (GV831_00120) - 19392..20171 (+) 780 WP_014570716.1 peptidoglycan amidohydrolase family protein -
  GV831_RS00135 (GV831_00130) rsmB 21125..22399 (+) 1275 WP_014570715.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  GV831_RS00140 (GV831_00135) - 22480..23256 (+) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  GV831_RS00145 (GV831_00140) pknB 23256..25139 (+) 1884 WP_003130801.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  GV831_RS00150 (GV831_00145) rpsO 25321..25590 (+) 270 WP_010906184.1 30S ribosomal protein S15 -
  GV831_RS00155 (GV831_00150) proC 25669..26457 (-) 789 WP_012898440.1 pyrroline-5-carboxylate reductase -
  GV831_RS00160 (GV831_00155) glmU 26685..28061 (+) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  GV831_RS00165 (GV831_00160) - 28166..28750 (+) 585 WP_003130797.1 NUDIX hydrolase -
  GV831_RS00170 (GV831_00165) macP 28743..29021 (+) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  GV831_RS00175 (GV831_00170) - 29054..29734 (+) 681 WP_003130794.1 5'-methylthioadenosine/adenosylhomocysteine nucleosidase -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67877.92 Da        Isoelectric Point: 4.7783

>NTDB_id=366366 GV831_RS00145 WP_003130801.1 23256..25139(+) (pknB) [Lactococcus lactis subsp. lactis strain WHH2311]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGSNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=366366 GV831_RS00145 WP_003130801.1 23256..25139(+) (pknB) [Lactococcus lactis subsp. lactis strain WHH2311]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTC
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATCTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTCTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-265)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(434-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CEF5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.265

86.762

0.427
  stkP/pknB Streptococcus salivarius strain HSISS4

51.445

82.775

0.426
  stkP Streptococcus pneumoniae TIGR4

48.024

80.702

0.388
  stkP Streptococcus pneumoniae D39

47.826

80.702

0.386
  stkP Streptococcus pneumoniae R6

47.826

80.702

0.386
  stkP Streptococcus pneumoniae Rx1

47.628

80.702

0.384