Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FEO95_RS18130 Genome accession   NZ_CP040432
Coordinates   3941826..3943559 (-) Length   577 a.a.
NCBI ID   WP_006387090.1    Uniprot ID   -
Organism   Stenotrophomonas maltophilia strain sm-RA9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3936826..3948559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FEO95_RS18115 (FEO95_18220) sucC 3937000..3938169 (-) 1170 WP_004154430.1 ADP-forming succinate--CoA ligase subunit beta -
  FEO95_RS18120 (FEO95_18225) - 3938546..3940159 (+) 1614 WP_032972469.1 ATP-binding protein -
  FEO95_RS18125 (FEO95_18230) pilR 3940220..3941593 (+) 1374 WP_006387002.1 sigma-54 dependent transcriptional regulator Regulator
  FEO95_RS18130 (FEO95_18235) pilB 3941826..3943559 (-) 1734 WP_006387090.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FEO95_RS18135 (FEO95_18240) - 3943904..3944776 (+) 873 WP_006387092.1 glycosyltransferase family 2 protein -
  FEO95_RS18140 (FEO95_18245) - 3944773..3946002 (+) 1230 WP_154354806.1 glycosyltransferase -
  FEO95_RS18145 (FEO95_18250) - 3945992..3947239 (+) 1248 WP_006387125.1 hypothetical protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62407.30 Da        Isoelectric Point: 6.0438

>NTDB_id=364383 FEO95_RS18130 WP_006387090.1 3941826..3943559(-) (pilB) [Stenotrophomonas maltophilia strain sm-RA9]
MNAVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKLVNASQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DEGMGNLDVAAGDEDLGASSGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPV
KLNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKL
FQDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCGNCKRRSTLPE
HALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKK
AAAGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=364383 FEO95_RS18130 WP_006387090.1 3941826..3943559(-) (pilB) [Stenotrophomonas maltophilia strain sm-RA9]
ATGAATGCCGTAACCACCGCCAACCTTGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGGGCCCTGGATGA
GGCCAGCGCCCGCGACGCCATGGCCAAGGCCGCCGCGGCCCGCCAGCCGTTGCCCACGTGGTTCGCGCAGAACAAGCTGG
TCAATGCCTCGCAGCTGGCCGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACCTTCGATGCCAGC
CAGAACGCGATGAGCCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAGCGTGGCGGCAAGCT
GTTTGTCGGTACCAGCAACCCGACCCACTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TCGACGAGGACCAGATCCGCCGCACGCTGGAGCAGTGGCACGCCAGTCATGACACCCTGGGCGATGCGCTGGGGGGCGAT
GACGAGGGCATGGGAAACCTGGATGTCGCAGCCGGTGACGAAGATCTCGGCGCCAGCAGCGGCGATTCCGGCATCGACGC
CAAGGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCATCGGACATCC
ATTTCGAGCCCTACGAAGACGATTACCGTGTGCGCCTGCGCATCGACGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTG
AAGTTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGATATCGCCGAGAAGCGTGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAGAAGG
TGGTACTGCGTATCCTCGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAGCAGAAGTTG
TTCCAGGATGCCATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGTAAGACCGTGTCGTTGTA
TACCGCGCTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACTGCCGAAGACCCGGTGGAAATCCGCCTGCCCGGCG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCTGCGGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGAC
ATCATCATGGTTGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCGCAGACCGGCCACATGGTGCT
GTCCACGCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCTTGATGAACATGGGCATCGCGCCGTACAACATCACCA
GCTCGGTGACGCTGGTGATCGCCCAGCGCCTGGCACGCCGCCTGTGCGGCAACTGCAAGCGGCGCTCGACCCTGCCGGAG
CATGCGCTGCTGGCCGAGGGCTTCACCCAGGCGCAGCTGGACGCGGGCATCGAGCTGTATGAAGCGGTGGGCTGCGATGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACTGATGAGATCGCCACCATCGTGCTGG
CTGGCGGCAACGCGTTGCAGATTGCGGAAGCAGCGCAGCAGATCGGGGTGAATGACCTGCGGCAGTCGGCATTGAAGAAG
GCGGCGGCAGGCGTGACCAGCCTGGCCGAGATCAATCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.345

100

0.556
  pilB Acinetobacter baumannii D1279779

54.889

100

0.555
  pilB Legionella pneumophila strain ERS1305867

51.93

98.787

0.513
  pilF Neisseria gonorrhoeae MS11

48.687

98.96

0.482
  pilB Vibrio cholerae strain A1552

48.336

98.96

0.478
  pilB Vibrio parahaemolyticus RIMD 2210633

45.583

98.094

0.447
  pilB Vibrio campbellii strain DS40M4

45.167

98.614

0.445

Multiple sequence alignment