Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   FAH66_RS07280 Genome accession   NZ_CP039887
Coordinates   1509414..1511093 (+) Length   559 a.a.
NCBI ID   WP_003685901.1    Uniprot ID   -
Organism   Neisseria subflava strain ATCC 49275     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1504414..1516093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FAH66_RS07260 (FAH66_07260) - 1504847..1505104 (+) 258 WP_070613328.1 SemiSWEET family transporter -
  FAH66_RS07270 (FAH66_07270) - 1505454..1505768 (+) 315 WP_080613331.1 hypothetical protein -
  FAH66_RS07275 (FAH66_07275) carB 1505795..1509010 (+) 3216 WP_137041171.1 carbamoyl-phosphate synthase large subunit -
  FAH66_RS07280 (FAH66_07280) pilF 1509414..1511093 (+) 1680 WP_003685901.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FAH66_RS07285 (FAH66_07285) pilG 1511173..1512414 (+) 1242 WP_137041172.1 type II secretion system F family protein Machinery gene
  FAH66_RS07290 (FAH66_07290) pilD 1512416..1513303 (+) 888 WP_137041173.1 A24 family peptidase Machinery gene
  FAH66_RS07295 (FAH66_07295) coaE 1513303..1513923 (+) 621 WP_137041174.1 dephospho-CoA kinase -
  FAH66_RS07300 (FAH66_07300) yacG 1513920..1514102 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  FAH66_RS07305 (FAH66_07305) glyQ 1514184..1515083 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  FAH66_RS07310 (FAH66_07310) lspA 1515232..1515738 (+) 507 WP_070633008.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62299.60 Da        Isoelectric Point: 6.2593

>NTDB_id=360948 FAH66_RS07280 WP_003685901.1 1509414..1511093(+) (pilF) [Neisseria subflava strain ATCC 49275]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPNLFSDGIISPKALGELVARVFSYPLLDLRYYPRNNVVSDILTEEQ
MVQNRCIPIFKRGRKVYFAVSDPTQIQKFQKIAFASGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINDEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDTDLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=360948 FAH66_RS07280 WP_003685901.1 1509414..1511093(+) (pilF) [Neisseria subflava strain ATCC 49275]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAGACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCAACCTGTTTTCAGACGGCATCATCTCCCCTAAAGCTTTGGGAGAGTTGGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCGCTACTACCCGCGCAATAATGTAGTCAGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCATTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACCCAAATCCA
AAAATTTCAAAAAATTGCTTTTGCTTCAGGTTTAAGTGTCGACTTGGTAGTAGTTCCAGACGACCAGCTCAGCTCATTGC
TGGAATGGCTAGGTCAACGTTCGACCACCATTCTGAAAGAAATCAACGACGAACATGAAGCAACGCAGCAATCGCAATCG
CTTTATATCGACAATGAAGAAGCTGAAGATGGTCCAATCCCCCGCTTCATCCATAAAACCTTATCCGATGCCCTGAACGC
GGGCGCATCGGATATCCACTTTGAATTCTACGAGCAAATGGCGCGCGTTCGTTTCCGCGTGGACGGACAATTGCGTGAAG
TCGTACAGCCTCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCCGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCATAAACACGGCCGTCCGATTGACTTCCGTGTCAGCACATTGCC
GACGTTGTTTGGCGAAAAAGTGGTAATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGCTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTGTTGGTTACCGGTCCGACAGGTTCG
GGCAAAACCGTTTCACTCTATACCTGCCTGAGTATTTTGAACACGGAAGATGTGAACATTTCGACGGCCGAGGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAGAAACAAGGTCTGACCTTTGCTGCCGCACTCCGATCCT
TCTTGCGCCAAGATCCTGATATTATTATGGTCGGTGAGATCCGAGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACCGGCCACATGGTTTTCTCGACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGCGT
AGCGCCTTTCAATATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTTGAACGCCCTCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATACGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGCTGCGACAGCTGCCGCGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACTTGCGTCGCGCCGGCCTCATGAAAGTCATGCAAGGCCTGACCTCGTTGGAAGAAGTGACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.639

100

0.776
  pilB Acinetobacter baylyi ADP1

51.056

100

0.519
  pilB Acinetobacter baumannii D1279779

50.442

100

0.51
  pilB Legionella pneumophila strain ERS1305867

46.996

100

0.476
  pilB Vibrio cholerae strain A1552

46.085

100

0.463
  pilB Vibrio campbellii strain DS40M4

44.602

100

0.451
  pilB Vibrio parahaemolyticus RIMD 2210633

46.729

95.707

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.361

96.064

0.369

Multiple sequence alignment