Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GUK18_RS12390 Genome accession   NZ_CP047600
Coordinates   2545087..2546568 (+) Length   493 a.a.
NCBI ID   WP_001219895.1    Uniprot ID   A0A0H3EQD7
Organism   Escherichia coli O84:H7 strain Trh52     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2540087..2551568
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GUK18_RS12365 (GUK18_12395) gspB 2540256..2540675 (-) 420 WP_000461443.1 putative general secretion pathway protein GspB -
  GUK18_RS12370 (GUK18_12400) gspA 2540677..2542146 (-) 1470 Protein_2450 type II secretion system protein GspA -
  GUK18_RS12380 (GUK18_12410) gspC 2542326..2543141 (+) 816 WP_024223340.1 type II secretion system protein GspC -
  GUK18_RS12385 (GUK18_12415) gspD 2543125..2545077 (+) 1953 WP_024223341.1 type II secretion system secretin GspD -
  GUK18_RS12390 (GUK18_12420) pilB 2545087..2546568 (+) 1482 WP_001219895.1 type II secretion system ATPase GspE Machinery gene
  GUK18_RS12395 (GUK18_12425) gspF 2546565..2547761 (+) 1197 WP_001109576.1 type II secretion system inner membrane protein GspF -
  GUK18_RS12400 (GUK18_12430) gspG 2547771..2548208 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  GUK18_RS12405 (GUK18_12435) gspH 2548216..2548725 (+) 510 WP_001076040.1 type II secretion system minor pseudopilin GspH -
  GUK18_RS12410 (GUK18_12440) gspI 2548722..2549099 (+) 378 WP_001041750.1 type II secretion system minor pseudopilin GspI -
  GUK18_RS12415 (GUK18_12445) gspJ 2549092..2549679 (+) 588 WP_000610638.1 type II secretion system minor pseudopilin GspJ -
  GUK18_RS12420 (GUK18_12450) gspK 2549672..2550655 (+) 984 WP_024223342.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 493 a.a.        Molecular weight: 54692.63 Da        Isoelectric Point: 6.2412

>NTDB_id=360339 GUK18_RS12390 WP_001219895.1 2545087..2546568(+) (pilB) [Escherichia coli O84:H7 strain Trh52]
MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQVGQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDI
DQSVDLLSLSEEMPANEDLLNEDSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQLSLNNLGMTAADKQDLENLIQL
PHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTVEDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEI
RDTETAQIAVQASLTGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSLAGIIAQRLVRRLCPQCRQFTPVSPQQAQMFK
YHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQALERLVRQQHNALIKNGLQKVIRGDTSWDE
VMRVASATLENEA

Nucleotide


Download         Length: 1482 bp        

>NTDB_id=360339 GUK18_RS12390 WP_001219895.1 2545087..2546568(+) (pilB) [Escherichia coli O84:H7 strain Trh52]
ATGAGAATTCACTCACCGTACCCCGCCAGTTGGGCGCTGGCACAACGAATTGGTTATCTCTATTCAGAGGGCGAGATTAT
TTATCTCGCCGATACGCCATTCGAACGGTTACTCGATATTCAACGTCAAGTTGGCCAGTGCCAGACCATGACCAGCTTGT
CACAGGCTGATTTTGAAGCCCGACTGGAAGCGGTGTTCCATCAGAATACCGGTGAGTCGCAACAGATTGCGCAGGATATC
GATCAATCCGTCGATCTTCTCTCGCTTTCGGAAGAGATGCCCGCAAATGAAGATCTCCTGAATGAAGATTCAGCGGCACC
GGTTATCCGCTTGATCAATGCTATTTTGAGTGAGGCCATCAAAGAAACCGCCTCCGATATCCACATTGAAACCTATGAAA
AAACAATGTCGATCCGTTTTCGCATCGACGGCGTTTTGCGGACAATTTTACAGCCCAACAAAAAACTGGCGGCACTGCTT
ATCTCCCGAATTAAGGTCATGGCTCGTCTTGATATCGCCGAAAAACGTATTCCACAGGATGGAAGAATCAGTTTGCGTAT
CGGGCGACGTAACATAGATGTCCGCGTATCCACACTGCCATCCATCTATGGTGAACGCGCTGTGCTCCGCCTGCTGGATA
AAAACAGCCTCCAGCTTTCATTGAACAACCTGGGGATGACGGCAGCGGATAAACAGGATTTAGAAAATCTCATTCAGCTT
CCGCACGGTATTATCCTGGTAACAGGGCCGACAGGCTCCGGTAAAAGCACCACGCTCTACGCCATTCTTTCGGCGCTTAA
TACCCCTGGCCGTAATATTCTGACGGTAGAAGATCCCGTGGAATATGAACTGGAAGGCATAGGACAAACGCAGGTGAATA
CCCGCGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAGATT
CGTGATACAGAAACCGCGCAAATTGCGGTTCAGGCCTCGCTCACAGGCCATCTGGTACTCTCAACACTCCACACTAACAG
TGCATCAGGCGCAGTCACCCGGCTACGCGACATGGGCGTCGAATCATTCCTGCTTTCGTCTTCACTGGCAGGGATTATCG
CGCAACGTCTGGTTCGTCGTCTGTGTCCGCAATGCCGACAATTCACGCCCGTATCGCCCCAGCAAGCGCAGATGTTTAAA
TATCATCAGCTCGCGGTGACGACAATTGGCACTCCCGTAGGCTGCCCTCATTGCCATCAATCCGGCTACCAGGGGCGCAT
GGCGATCCACGAAATGATGGTGGTGACGCCGGAATTGCGGGCCGCTATTCATGAAAATGTGGATGAACAAGCACTGGAGC
GACTGGTCCGGCAACAACACAATGCCTTAATCAAAAATGGCCTGCAAAAAGTGATACGCGGTGACACCTCCTGGGATGAG
GTTATGCGCGTCGCCAGTGCCACGCTGGAGAACGAAGCATGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(114-380)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H3EQD7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

46.682

88.641

0.414
  pilB Vibrio cholerae strain A1552

48.441

84.584

0.41
  pilB Acinetobacter baumannii D1279779

45.682

89.249

0.408
  pilB Legionella pneumophila strain ERS1305867

41.372

97.566

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

46.353

86.207

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

41.202

94.523

0.389
  pilB Acinetobacter baylyi ADP1

48.593

79.31

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.178

79.919

0.361
  pilF Thermus thermophilus HB27

45.063

80.122

0.361

Multiple sequence alignment