Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VB87_RS03785 Genome accession   NC_013722
Coordinates   722473..724200 (+) Length   575 a.a.
NCBI ID   WP_012915195.1    Uniprot ID   D2UBN4
Organism   Xanthomonas albilineans GPE PC73     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 717473..729200
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VB87_RS03765 (XALC_0663) coaE 718743..719366 (-) 624 WP_012915191.1 dephospho-CoA kinase -
  VB87_RS03770 (XALC_0664) - 719379..720242 (-) 864 WP_012915192.1 A24 family peptidase -
  VB87_RS03775 (XALC_0665) pilC 720249..721520 (-) 1272 WP_012915193.1 type II secretion system F family protein Machinery gene
  VB87_RS03780 (XALC_0666) - 721886..722323 (+) 438 WP_012915194.1 pilin -
  VB87_RS03785 (XALC_0667) pilB 722473..724200 (+) 1728 WP_012915195.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VB87_RS03790 (XALC_0668) pilR 724344..725732 (-) 1389 WP_012915196.1 sigma-54 dependent transcriptional regulator Regulator
  VB87_RS03795 (XALC_0669) - 725820..727433 (-) 1614 WP_012915197.1 ATP-binding protein -
  VB87_RS03800 (XALC_0670) sucC 727724..728887 (+) 1164 WP_012915198.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62509.65 Da        Isoelectric Point: 5.9527

>NTDB_id=35976 VB87_RS03785 WP_012915195.1 722473..724200(+) (pilB) [Xanthomonas albilineans GPE PC73]
MSAISANLVGITGIARRLVQDGVLEEAAARAAMAQATEARVPLPQWFAEKKLVTAAQLAAANAVEFGVPLLDASVFDPSQ
NAMKLVSEELLHKHQVLPLFKRGSRLFVGISNPTQTRALDDIKFHTNLTVEPILVDEDQIRRTLEQWHSSNDALGSALGG
DDEGMDNLDVGGDEDGGSGDTGMDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKL
NQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKNKQIDFRVSTLPTLFGEKIVLRILDGSAAKLGIDKLGYEPEQQQLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDII
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPVKLPDKA
LLAEGFTEADIAAGIQLYEPVGCEECTEGYKGRTGIYQVMPMNDEIAAIVLQGGNAMDIASAAQKIGVNDLRQSALLKAR
NGITSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=35976 VB87_RS03785 WP_012915195.1 722473..724200(+) (pilB) [Xanthomonas albilineans GPE PC73]
ATGAGCGCCATATCAGCCAATCTCGTCGGCATCACCGGCATCGCCCGCCGTCTCGTTCAGGATGGGGTCTTGGAGGAGGC
CGCGGCGCGGGCCGCCATGGCCCAGGCGACCGAGGCCAGGGTCCCGCTGCCGCAATGGTTTGCCGAGAAGAAGCTGGTCA
CCGCTGCGCAGTTGGCCGCCGCCAATGCGGTGGAATTCGGCGTGCCATTGCTGGACGCCTCGGTGTTCGACCCCAGCCAG
AACGCGATGAAGCTGGTCAGCGAGGAACTGCTGCACAAGCACCAGGTGCTGCCGTTGTTCAAGCGCGGTAGCCGGCTGTT
CGTGGGGATCAGCAATCCCACCCAGACCCGCGCGCTGGACGACATCAAGTTCCATACCAATCTCACCGTCGAGCCGATCC
TGGTCGATGAGGACCAGATCCGGCGCACGCTGGAGCAGTGGCATTCCAGCAACGATGCGCTCGGCAGTGCGTTGGGCGGC
GACGACGAGGGCATGGACAACCTCGATGTGGGCGGCGACGAGGACGGCGGCAGCGGCGACACCGGTATGGACGCCAAGAG
CGACGACACGCCGGTGGTCAAGTTCGTCAACAAGGTGCTGATCGATGCGATCCGCCGCGGTGCCTCGGATATCCATTTCG
AGCCCTACGAGGACGACTACCGGGTGCGCCTGCGCATCGATGGCCTGCTCAAGAACGTGGCCAAGGCGCCGGTCAAGCTC
AATCAGCGCATCGCCGCGCGGCTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAGCGGGTGCCGCAGGACGGGCGCAT
CAAGCTCAATCTGTCCAAGAACAAGCAGATCGACTTCCGCGTCAGCACACTGCCGACGCTGTTCGGCGAGAAGATCGTGC
TGCGTATTCTCGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTCGGCTACGAGCCGGAACAGCAGCAGTTGTTCCTG
GACGCGATCCATAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGCTCCGGCAAGACGGTGTCGCTGTATACCGC
GCTGGGCATTCTCAACGACGAAACCCGCAACATTTCCACCGCCGAGGACCCGGTGGAAATCCGTCTGCCGGGCGTCAATC
AGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCAGCCTTGCGTTCGTTCCTGCGGCAGGACCCTGACATCATC
ATGGTCGGCGAAATCCGCGACTTGGAAACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
CCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATCGCCCCCTACAACATCACCAGTTCGG
TGACCTTGGTGATCGCGCAGCGTCTGGCGCGGCGGCTGTGCAACAACTGCAAACGCCCGGTCAAATTGCCGGATAAAGCC
TTGCTGGCCGAAGGCTTCACCGAAGCGGACATCGCCGCGGGGATCCAGTTGTACGAGCCGGTCGGTTGCGAGGAATGCAC
CGAAGGCTACAAGGGCCGCACCGGCATCTACCAGGTGATGCCGATGAACGACGAAATCGCCGCGATCGTGCTGCAGGGCG
GTAACGCGATGGACATCGCCAGCGCCGCGCAGAAGATCGGGGTCAATGATCTGCGTCAGTCGGCCTTACTCAAGGCGCGC
AATGGCATCACCAGTTTGGCTGAGATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB D2UBN4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.382

100

0.555
  pilB Acinetobacter baylyi ADP1

56.007

98.435

0.551
  pilB Legionella pneumophila strain ERS1305867

51.408

98.783

0.508
  pilB Vibrio cholerae strain A1552

49.301

99.478

0.49
  pilF Neisseria gonorrhoeae MS11

48.858

98.957

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.986

98.087

0.461
  pilB Vibrio campbellii strain DS40M4

46.06

99.304

0.457
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.808

90.435

0.36

Multiple sequence alignment