Detailed information NTG0358197

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EA665_RS15150
• Genome accession: NZ_CP039343
• Coordinates: 3131140..3132258 (+)
• Length: 372 a.a.
• NCBI ID: WP_024432390.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii strain TG31302
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3126140..3137258

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EA665_RS15140 (EA665_015140)	-	3129256..3129948 (-)	693	WP_024432389.1	YggS family pyridoxal phosphate-dependent enzyme	-
EA665_RS15145 (EA665_015145)	pilT	3130075..3131112 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EA665_RS15150 (EA665_015150)	pilU	3131140..3132258 (+)	1119	WP_024432390.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EA665_RS15155 (EA665_015155)	fur	3132328..3132765 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EA665_RS15160 (EA665_015160)	-	3132877..3133275 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EA665_RS15165 (EA665_015165)	-	3133330..3133653 (-)	324	WP_000442585.1	RnfH family protein	-
EA665_RS15170 (EA665_015170)	-	3133653..3134720 (-)	1068	WP_029776981.1	membrane protein	-
EA665_RS15180 (EA665_015180)	-	3134873..3135328 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EA665_RS15185 (EA665_015185)	-	3135577..3136425 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41889.11 Da
• Isoelectric Point: 6.6945

>NTDB_id=358197 EA665_RS15150 WP_024432390.1 3131140..3132258(+) (pilU) [Acinetobacter baumannii strain TG31302]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDFAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=358197 EA665_RS15150 WP_024432390.1 3131140..3132258(+) (pilU) [Acinetobacter baumannii strain TG31302]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
TCGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392